ALTERATIONS IN PERIPHERAL BLOOD CD8+ T CELL SUBSETS IN PATIENTS WITH LUNG SARCOIDOSIS
AbstractSarcoidosis is a systemic inflammatory disorder of unknown etiology characterized by tissue infiltration with macrophages and lymphocytes, including CD8+ T cells, and associated non-caseating granuloma formation. The aim of the study was to investigate various peripheral blood CD8+ T cells f...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2019-08-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/3222 |
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| author | Artem Arkad’evich Rubinstein Igor Vladimirovich Kudryavtsev Natalia Mihailovna Lazareva Tatyana Viktorovna Akisheva Olga Petrovna Baranova Tatyana Pavlovna Ses’ Mikhail Mikhailovich Ilkovich Areg Artemovich Totolian |
| author_facet | Artem Arkad’evich Rubinstein Igor Vladimirovich Kudryavtsev Natalia Mihailovna Lazareva Tatyana Viktorovna Akisheva Olga Petrovna Baranova Tatyana Pavlovna Ses’ Mikhail Mikhailovich Ilkovich Areg Artemovich Totolian |
| author_sort | Artem Arkad’evich Rubinstein |
| collection | DOAJ |
| description | AbstractSarcoidosis is a systemic inflammatory disorder of unknown etiology characterized by tissue infiltration with macrophages and lymphocytes, including CD8+ T cells, and associated non-caseating granuloma formation. The aim of the study was to investigate various peripheral blood CD8+ T cells from patients with chronic respiratory sarcoidosis using markers of T cell maturation and ‘polarization’. Peripheral blood samples were collected from 34 patients with newly diagnosed chronic sarcoidosis of the respiratory organs with the background of a natural course of disease and without immunosuppressive therapy history. The diagnosis of pulmonary sarcoidosis was performed according to the standard criteria and was confirmed by histological examination for 94,12% of patients. Peripheral venous blood samples from healthy volunteers (n=40), matched by gender and age with patients with pulmonary sarcoidosis, was used as a control group. Multicolor flow cytometry revealed that patients with sarcoidosis had decreased levels of CD45RA+CD62L+ ‘naïve’ and CD45RA–CD62L+ central memory CD8+ T cells if compared with healthy controls, as well as the frequencies of ЕМ1 (CD45RA–CD62L–CD27+CD28+) и pre-effector type 1 (CD45RA+CD62L–CD27+CD28+) cells were also reduced. Next, to assess relevant ‘polarized’ CD8+ T cell subsets, we identified Tc1 (CCR6–CXCR3+), Tc2 (CCR6–CXCR3–), Tc17 (CCR6+CXCR3–), and double-positive Tc17.1 (CCR6+CXCR3+). We found that CXCR3-expressing CD8+ T cell subsets (Tc1 and Tc17.1) were significantly decreased both in relative and absolute numbers in patients with sarcoidosis if compared to healthy controls. Oppositely, Тс2 CD8+ T cell were significantly elevated. Furthermore, the relative numbers of Tc1 cells negatively correlated with serum ACE levels (r=–0,456 with р=0,010), while Тс2 levels positively correlated with serum ACE levels (r=0,623 with р<0,001). Thus, our results indicate that CD8+ T cells may play a role in the pathogenesis of sarcoidosis. More extensive clinical and immunological comparisons are required for further systematization of the obtained data. |
| format | Article |
| id | doaj-art-d0b33a607954432a9e7a80d8976d38c3 |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2019-08-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-d0b33a607954432a9e7a80d8976d38c32025-08-20T04:00:26ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2019-08-010010.15789/1563-0625-AIP-32222030ALTERATIONS IN PERIPHERAL BLOOD CD8+ T CELL SUBSETS IN PATIENTS WITH LUNG SARCOIDOSISArtem Arkad’evich Rubinstein0Igor Vladimirovich Kudryavtsev1Natalia Mihailovna Lazareva2Tatyana Viktorovna Akisheva3Olga Petrovna Baranova4Tatyana Pavlovna Ses’5Mikhail Mikhailovich Ilkovich6Areg Artemovich Totolian7Institute of Experimental Medicine, St.Petersburg, Russian FederationInstitute of Experimental Medicine, St.Petersburg, Russian Federation; First St. Petersburg State I. Pavlov Medical University, St.Petersburg, Russian FederationFederal State Budgetary Institution "Russian Research Institute of Hematology and Transfusiology of the Federal Medical and Biological Agency", St.Petersburg, Russian FederationInstitute of Experimental Medicine, St.Petersburg, Russian FederationFirst St. Petersburg State I. Pavlov Medical University, St.Petersburg, Russian FederationFirst St. Petersburg State I. Pavlov Medical University, St.Petersburg, Russian FederationFirst St. Petersburg State I. Pavlov Medical University, St.Petersburg, Russian FederationFirst St. Petersburg State I. Pavlov Medical University, St.Petersburg, Russian Federation; St. Petersburg Pasteur Institute, St.Petersburg, Russian FederationAbstractSarcoidosis is a systemic inflammatory disorder of unknown etiology characterized by tissue infiltration with macrophages and lymphocytes, including CD8+ T cells, and associated non-caseating granuloma formation. The aim of the study was to investigate various peripheral blood CD8+ T cells from patients with chronic respiratory sarcoidosis using markers of T cell maturation and ‘polarization’. Peripheral blood samples were collected from 34 patients with newly diagnosed chronic sarcoidosis of the respiratory organs with the background of a natural course of disease and without immunosuppressive therapy history. The diagnosis of pulmonary sarcoidosis was performed according to the standard criteria and was confirmed by histological examination for 94,12% of patients. Peripheral venous blood samples from healthy volunteers (n=40), matched by gender and age with patients with pulmonary sarcoidosis, was used as a control group. Multicolor flow cytometry revealed that patients with sarcoidosis had decreased levels of CD45RA+CD62L+ ‘naïve’ and CD45RA–CD62L+ central memory CD8+ T cells if compared with healthy controls, as well as the frequencies of ЕМ1 (CD45RA–CD62L–CD27+CD28+) и pre-effector type 1 (CD45RA+CD62L–CD27+CD28+) cells were also reduced. Next, to assess relevant ‘polarized’ CD8+ T cell subsets, we identified Tc1 (CCR6–CXCR3+), Tc2 (CCR6–CXCR3–), Tc17 (CCR6+CXCR3–), and double-positive Tc17.1 (CCR6+CXCR3+). We found that CXCR3-expressing CD8+ T cell subsets (Tc1 and Tc17.1) were significantly decreased both in relative and absolute numbers in patients with sarcoidosis if compared to healthy controls. Oppositely, Тс2 CD8+ T cell were significantly elevated. Furthermore, the relative numbers of Tc1 cells negatively correlated with serum ACE levels (r=–0,456 with р=0,010), while Тс2 levels positively correlated with serum ACE levels (r=0,623 with р<0,001). Thus, our results indicate that CD8+ T cells may play a role in the pathogenesis of sarcoidosis. More extensive clinical and immunological comparisons are required for further systematization of the obtained data.https://www.mimmun.ru/mimmun/article/view/3222sarcoidosiscd8+ t cellst cell maturationtс1 cell subsetstс17th17.1flow cytometry |
| spellingShingle | Artem Arkad’evich Rubinstein Igor Vladimirovich Kudryavtsev Natalia Mihailovna Lazareva Tatyana Viktorovna Akisheva Olga Petrovna Baranova Tatyana Pavlovna Ses’ Mikhail Mikhailovich Ilkovich Areg Artemovich Totolian ALTERATIONS IN PERIPHERAL BLOOD CD8+ T CELL SUBSETS IN PATIENTS WITH LUNG SARCOIDOSIS Медицинская иммунология sarcoidosis cd8+ t cells t cell maturation tс1 cell subsets tс17 th17.1 flow cytometry |
| title | ALTERATIONS IN PERIPHERAL BLOOD CD8+ T CELL SUBSETS IN PATIENTS WITH LUNG SARCOIDOSIS |
| title_full | ALTERATIONS IN PERIPHERAL BLOOD CD8+ T CELL SUBSETS IN PATIENTS WITH LUNG SARCOIDOSIS |
| title_fullStr | ALTERATIONS IN PERIPHERAL BLOOD CD8+ T CELL SUBSETS IN PATIENTS WITH LUNG SARCOIDOSIS |
| title_full_unstemmed | ALTERATIONS IN PERIPHERAL BLOOD CD8+ T CELL SUBSETS IN PATIENTS WITH LUNG SARCOIDOSIS |
| title_short | ALTERATIONS IN PERIPHERAL BLOOD CD8+ T CELL SUBSETS IN PATIENTS WITH LUNG SARCOIDOSIS |
| title_sort | alterations in peripheral blood cd8 t cell subsets in patients with lung sarcoidosis |
| topic | sarcoidosis cd8+ t cells t cell maturation tс1 cell subsets tс17 th17.1 flow cytometry |
| url | https://www.mimmun.ru/mimmun/article/view/3222 |
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