Genetic manipulation of rod-cone differences in mouse retina.
Though rod and cone photoreceptors use similar phototransduction mechanisms, previous model calculations have indicated that the most important differences in their light responses are likely to be differences in amplification of the G-protein cascade, different decay rates of phosphodiesterase (PDE...
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0300584&type=printable |
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| author | Ala Morshedian Zhichun Jiang Roxana A Radu Gordon L Fain Alapakkam P Sampath |
| author_facet | Ala Morshedian Zhichun Jiang Roxana A Radu Gordon L Fain Alapakkam P Sampath |
| author_sort | Ala Morshedian |
| collection | DOAJ |
| description | Though rod and cone photoreceptors use similar phototransduction mechanisms, previous model calculations have indicated that the most important differences in their light responses are likely to be differences in amplification of the G-protein cascade, different decay rates of phosphodiesterase (PDE) and pigment phosphorylation, and different rates of turnover of cGMP in darkness. To test this hypothesis, we constructed TrUx;GapOx rods by crossing mice with decreased transduction gain from decreased transducin expression, with mice displaying an increased rate of PDE decay from increased expression of GTPase-activating proteins (GAPs). These two manipulations brought the sensitivity of TrUx;GapOx rods to within a factor of 2 of WT cone sensitivity, after correcting for outer-segment dimensions. These alterations did not, however, change photoreceptor adaptation: rods continued to show increment saturation though at a higher background intensity. These experiments confirm model calculations that rod responses can mimic some (though not all) of the features of cone responses after only a few changes in the properties of transduction proteins. |
| format | Article |
| id | doaj-art-d0303dcee5a74ef18ee94187829a15a0 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-d0303dcee5a74ef18ee94187829a15a02024-11-21T05:31:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01195e030058410.1371/journal.pone.0300584Genetic manipulation of rod-cone differences in mouse retina.Ala MorshedianZhichun JiangRoxana A RaduGordon L FainAlapakkam P SampathThough rod and cone photoreceptors use similar phototransduction mechanisms, previous model calculations have indicated that the most important differences in their light responses are likely to be differences in amplification of the G-protein cascade, different decay rates of phosphodiesterase (PDE) and pigment phosphorylation, and different rates of turnover of cGMP in darkness. To test this hypothesis, we constructed TrUx;GapOx rods by crossing mice with decreased transduction gain from decreased transducin expression, with mice displaying an increased rate of PDE decay from increased expression of GTPase-activating proteins (GAPs). These two manipulations brought the sensitivity of TrUx;GapOx rods to within a factor of 2 of WT cone sensitivity, after correcting for outer-segment dimensions. These alterations did not, however, change photoreceptor adaptation: rods continued to show increment saturation though at a higher background intensity. These experiments confirm model calculations that rod responses can mimic some (though not all) of the features of cone responses after only a few changes in the properties of transduction proteins.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0300584&type=printable |
| spellingShingle | Ala Morshedian Zhichun Jiang Roxana A Radu Gordon L Fain Alapakkam P Sampath Genetic manipulation of rod-cone differences in mouse retina. PLoS ONE |
| title | Genetic manipulation of rod-cone differences in mouse retina. |
| title_full | Genetic manipulation of rod-cone differences in mouse retina. |
| title_fullStr | Genetic manipulation of rod-cone differences in mouse retina. |
| title_full_unstemmed | Genetic manipulation of rod-cone differences in mouse retina. |
| title_short | Genetic manipulation of rod-cone differences in mouse retina. |
| title_sort | genetic manipulation of rod cone differences in mouse retina |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0300584&type=printable |
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