Scaling up production of cephalosporin C by Acremonium chrysogenum W42-I in a fermenter using submerged fermentation
Abstract Cephalosporins presently stand as the most extensively utilized antibiotic in clinical settings. Acremonium (A.) chrysogenum is the main strain used in the manufacturing of cephalosporin C (CPC), which offers distinct advantages, including a wide-ranging antibacterial spectrum and powerful...
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2024-11-01
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| Online Access: | https://doi.org/10.1186/s13568-024-01778-1 |
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| author | Asmaa A. Ibrahim Ghadir S. El-Housseiny Khaled M. Aboshanab Ansgar Stratmann Mahmoud A. Yassien Nadia A. Hassouna |
| author_facet | Asmaa A. Ibrahim Ghadir S. El-Housseiny Khaled M. Aboshanab Ansgar Stratmann Mahmoud A. Yassien Nadia A. Hassouna |
| author_sort | Asmaa A. Ibrahim |
| collection | DOAJ |
| description | Abstract Cephalosporins presently stand as the most extensively utilized antibiotic in clinical settings. Acremonium (A.) chrysogenum is the main strain used in the manufacturing of cephalosporin C (CPC), which offers distinct advantages, including a wide-ranging antibacterial spectrum and powerful antibacterial efficacy. Our study aimed to determine the optimal conditions for scaling up the production of CPC from A. chrysogenum W42-I starting with the optimized conditions on the shake flask level obtained from our previous study and utilizing the optimized media (CPC2). The results indicated that an inoculum size equivalent to 1% v/v, aeration at 1 vvm, and an agitation rate of 400 rpm, with controlled pH at 4, were the most favorable conditions for the CPC production using a laboratory fermentor (14 L). The concentration of generated CPC was assessed using two standard curves obtained from agar well diffusion and high-performance liquid chromatography (HPLC). These optimized conditions resulted in a production of 399.52 µg/mL showing a significant increase of approximately 3.4 folds when compared to the unoptimized fermentation run. In conclusion, our findings demonstrated a more favorable time course for CPC production in the fermentor compared to that in the shake flask. Notably, there was a two-fold increase in production within the first three days. Fortunately, the fermentor achieved a noteworthy increase in output, generating 1.598 gm of the CPC within 4 L. |
| format | Article |
| id | doaj-art-d004f5bbf5e64ab5a535ba9e29e6d67d |
| institution | Kabale University |
| issn | 2191-0855 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj-art-d004f5bbf5e64ab5a535ba9e29e6d67d2024-11-10T12:43:43ZengSpringerOpenAMB Express2191-08552024-11-0114111210.1186/s13568-024-01778-1Scaling up production of cephalosporin C by Acremonium chrysogenum W42-I in a fermenter using submerged fermentationAsmaa A. Ibrahim0Ghadir S. El-Housseiny1Khaled M. Aboshanab2Ansgar Stratmann3Mahmoud A. Yassien4Nadia A. Hassouna5Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams UniversityDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams UniversityDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams UniversityW42 Industrial Biotechnology GmbHDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams UniversityDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams UniversityAbstract Cephalosporins presently stand as the most extensively utilized antibiotic in clinical settings. Acremonium (A.) chrysogenum is the main strain used in the manufacturing of cephalosporin C (CPC), which offers distinct advantages, including a wide-ranging antibacterial spectrum and powerful antibacterial efficacy. Our study aimed to determine the optimal conditions for scaling up the production of CPC from A. chrysogenum W42-I starting with the optimized conditions on the shake flask level obtained from our previous study and utilizing the optimized media (CPC2). The results indicated that an inoculum size equivalent to 1% v/v, aeration at 1 vvm, and an agitation rate of 400 rpm, with controlled pH at 4, were the most favorable conditions for the CPC production using a laboratory fermentor (14 L). The concentration of generated CPC was assessed using two standard curves obtained from agar well diffusion and high-performance liquid chromatography (HPLC). These optimized conditions resulted in a production of 399.52 µg/mL showing a significant increase of approximately 3.4 folds when compared to the unoptimized fermentation run. In conclusion, our findings demonstrated a more favorable time course for CPC production in the fermentor compared to that in the shake flask. Notably, there was a two-fold increase in production within the first three days. Fortunately, the fermentor achieved a noteworthy increase in output, generating 1.598 gm of the CPC within 4 L.https://doi.org/10.1186/s13568-024-01778-1Cephalosporin CScaling upFermentorAcremonium chrysogenum |
| spellingShingle | Asmaa A. Ibrahim Ghadir S. El-Housseiny Khaled M. Aboshanab Ansgar Stratmann Mahmoud A. Yassien Nadia A. Hassouna Scaling up production of cephalosporin C by Acremonium chrysogenum W42-I in a fermenter using submerged fermentation AMB Express Cephalosporin C Scaling up Fermentor Acremonium chrysogenum |
| title | Scaling up production of cephalosporin C by Acremonium chrysogenum W42-I in a fermenter using submerged fermentation |
| title_full | Scaling up production of cephalosporin C by Acremonium chrysogenum W42-I in a fermenter using submerged fermentation |
| title_fullStr | Scaling up production of cephalosporin C by Acremonium chrysogenum W42-I in a fermenter using submerged fermentation |
| title_full_unstemmed | Scaling up production of cephalosporin C by Acremonium chrysogenum W42-I in a fermenter using submerged fermentation |
| title_short | Scaling up production of cephalosporin C by Acremonium chrysogenum W42-I in a fermenter using submerged fermentation |
| title_sort | scaling up production of cephalosporin c by acremonium chrysogenum w42 i in a fermenter using submerged fermentation |
| topic | Cephalosporin C Scaling up Fermentor Acremonium chrysogenum |
| url | https://doi.org/10.1186/s13568-024-01778-1 |
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