Systematic Survey and Analysis Reveal Jasmonate ZIM-Domain Gene Family in <i>Coix lacryma-jobi</i> Under High Temperature
Jasmonate ZIM-domain (JAZ) acts as the repressor of the JA signaling pathway and plays a significant role in stress-inducible defense, hormone crosstalk, and the regulation of the growth-defense tradeoff. The aim of this study is to systematically survey and analyze the JAZ gene family in <i>C...
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2024-11-01
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| author | Zhenming Yu Yufeng Shen Yiming Sun Zhangting Xu Feixiong Zheng Xiaoxia Shen |
| author_facet | Zhenming Yu Yufeng Shen Yiming Sun Zhangting Xu Feixiong Zheng Xiaoxia Shen |
| author_sort | Zhenming Yu |
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| description | Jasmonate ZIM-domain (JAZ) acts as the repressor of the JA signaling pathway and plays a significant role in stress-inducible defense, hormone crosstalk, and the regulation of the growth-defense tradeoff. The aim of this study is to systematically survey and analyze the JAZ gene family in <i>Coix lacryma-jobi</i> and unveil its expression profiles in diverse organs under high-temperature stress using transcriptome. The results identified a total of 20 JAZ family proteins randomly mapped on four chromosomes and encoding 159–409 amino acids. They were clustered into six groups and were mainly located in the nucleus. The conserved motifs, gene composition, and secondary structure of ClJAZ members within the same subtribes were similar. Multitudinous <i>cis</i>-regulating elements employed in hormone responsiveness and stress responsiveness were displayed before the promoter sequences of <i>ClJAZ1</i>-<i>ClJAZ20</i>. <i>ClJAZ1</i>-<i>ClJAZ20</i> were differentially distributed across diverse organs (the roots, shoots, leaves, kernels, glumes, and flowers), exposed to high-temperature stresses, and treated using ABA or MeJA. A total of 29115 DEGs were identified under heat stress, which were mainly involved in biological regulation and the metabolic process. Intriguingly, <i>ClJAZ15</i> was highly expressed in the leaves of <i>C. lacryma-jobi</i>, down-regulated by MeJA, but up-regulated by heat stress and ABA, inferring that <i>ClJAZ15</i> might be associated with ABA-inducible heat stress. The results laid a foundation for in-depth study of the role of ClJAZ family genes in <i>C. lacryma-jobi</i>. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-cfd1a6e4a4544ef8ae10cf60e02efb7b2024-11-26T18:19:02ZengMDPI AGPlants2223-77472024-11-011322323010.3390/plants13223230Systematic Survey and Analysis Reveal Jasmonate ZIM-Domain Gene Family in <i>Coix lacryma-jobi</i> Under High TemperatureZhenming Yu0Yufeng Shen1Yiming Sun2Zhangting Xu3Feixiong Zheng4Xiaoxia Shen5School of Pharmaceutical Sciences, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaJasmonate ZIM-domain (JAZ) acts as the repressor of the JA signaling pathway and plays a significant role in stress-inducible defense, hormone crosstalk, and the regulation of the growth-defense tradeoff. The aim of this study is to systematically survey and analyze the JAZ gene family in <i>Coix lacryma-jobi</i> and unveil its expression profiles in diverse organs under high-temperature stress using transcriptome. The results identified a total of 20 JAZ family proteins randomly mapped on four chromosomes and encoding 159–409 amino acids. They were clustered into six groups and were mainly located in the nucleus. The conserved motifs, gene composition, and secondary structure of ClJAZ members within the same subtribes were similar. Multitudinous <i>cis</i>-regulating elements employed in hormone responsiveness and stress responsiveness were displayed before the promoter sequences of <i>ClJAZ1</i>-<i>ClJAZ20</i>. <i>ClJAZ1</i>-<i>ClJAZ20</i> were differentially distributed across diverse organs (the roots, shoots, leaves, kernels, glumes, and flowers), exposed to high-temperature stresses, and treated using ABA or MeJA. A total of 29115 DEGs were identified under heat stress, which were mainly involved in biological regulation and the metabolic process. Intriguingly, <i>ClJAZ15</i> was highly expressed in the leaves of <i>C. lacryma-jobi</i>, down-regulated by MeJA, but up-regulated by heat stress and ABA, inferring that <i>ClJAZ15</i> might be associated with ABA-inducible heat stress. The results laid a foundation for in-depth study of the role of ClJAZ family genes in <i>C. lacryma-jobi</i>.https://www.mdpi.com/2223-7747/13/22/3230<i>Coix lacryma-jobi</i>JAZtranscriptomehigh-temperatureABA |
| spellingShingle | Zhenming Yu Yufeng Shen Yiming Sun Zhangting Xu Feixiong Zheng Xiaoxia Shen Systematic Survey and Analysis Reveal Jasmonate ZIM-Domain Gene Family in <i>Coix lacryma-jobi</i> Under High Temperature Plants <i>Coix lacryma-jobi</i> JAZ transcriptome high-temperature ABA |
| title | Systematic Survey and Analysis Reveal Jasmonate ZIM-Domain Gene Family in <i>Coix lacryma-jobi</i> Under High Temperature |
| title_full | Systematic Survey and Analysis Reveal Jasmonate ZIM-Domain Gene Family in <i>Coix lacryma-jobi</i> Under High Temperature |
| title_fullStr | Systematic Survey and Analysis Reveal Jasmonate ZIM-Domain Gene Family in <i>Coix lacryma-jobi</i> Under High Temperature |
| title_full_unstemmed | Systematic Survey and Analysis Reveal Jasmonate ZIM-Domain Gene Family in <i>Coix lacryma-jobi</i> Under High Temperature |
| title_short | Systematic Survey and Analysis Reveal Jasmonate ZIM-Domain Gene Family in <i>Coix lacryma-jobi</i> Under High Temperature |
| title_sort | systematic survey and analysis reveal jasmonate zim domain gene family in i coix lacryma jobi i under high temperature |
| topic | <i>Coix lacryma-jobi</i> JAZ transcriptome high-temperature ABA |
| url | https://www.mdpi.com/2223-7747/13/22/3230 |
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