Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer Cells
Hepatocellular carcinoma (HCC) with high epidemiological report data. Pathogenesis in HCC also involves several signaling pathways. This study aims to evaluate the in vitro activity of Moringa seed NanoChitosan against Hep G2 liver cancer cells and Co-Treatment with Doxorubicin. Initially, nanopart...
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Bogor Agricultural University
2024-12-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/58913 |
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| author | Hendra Susanto Moch Sholeh Agustina Tri Endharti Nik Ahmad Nizam Nik Malek Sa’diyatul Rizqie Amaliyah Firdaus Adeodatus Yuda Handaya Ahmad Taufiq |
| author_facet | Hendra Susanto Moch Sholeh Agustina Tri Endharti Nik Ahmad Nizam Nik Malek Sa’diyatul Rizqie Amaliyah Firdaus Adeodatus Yuda Handaya Ahmad Taufiq |
| author_sort | Hendra Susanto |
| collection | DOAJ |
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Hepatocellular carcinoma (HCC) with high epidemiological report data. Pathogenesis in HCC also involves several signaling pathways. This study aims to evaluate the in vitro activity of Moringa seed NanoChitosan against Hep G2 liver cancer cells and Co-Treatment with Doxorubicin. Initially, nanoparticles were prepared by extracting Moringa seeds, formulating them into nano chitosan, and then characterizing the compounds and particle sizes. The IC50 dose was investigated using the MTT assay. Then, the IC50 dose was confirmed in more detail through immunofluorescence, betatrophin gene, several genes in the Wnt-βcatenin-CyclinD1 proliferation pathway, and the addition of the apoptotic effector Caspase-3 using RT-qPCR analysis. Each treatment used a single dose of NCH-Mosee and co-treatment or combination with 4 μg/ml doxorubicin. The IC50 dose was 994 μg/ml in single treatment and 649 μg/ml in combined treatment with Dox. Hep G2 showed a decrease in the expression level of each parameter measured with increasing single dose and combination treatment (p < 0.050). Histologically, cells shrank, betatrophin expression was inhibited, and luminescence was seen, which decreased with increasing dose. In conclusion, NCH-Mosee with dose-tracking toxicity combined with Dox can suppress the viability of Hep G2 cells.
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| format | Article |
| id | doaj-art-cf9376ce42704bfca35bea0d3ae16e8e |
| institution | Kabale University |
| issn | 1978-3019 2086-4094 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj-art-cf9376ce42704bfca35bea0d3ae16e8e2025-01-08T09:00:42ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942024-12-0132210.4308/hjb.32.2.414-425Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer CellsHendra Susanto0Moch Sholeh1Agustina Tri Endharti2Nik Ahmad Nizam Nik Malek3Sa’diyatul Rizqie Amaliyah Firdaus4Adeodatus Yuda Handaya5Ahmad Taufiq6Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, 65114, IndonesiaDepartment of Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya, Malang, 65145, Indonesia Department of Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya, Malang, 65145, Indonesia Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia (UTM), Johor, 81310, MalaysiaDepartment of Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya, Malang, 65145, Indonesia Division of Digestive Surgeon, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Dr.Sardjito General Hospital Yogyakarta, Yogyakarta, 55281, IndonesiaDepartment of Physics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, 65114, Indonesia Hepatocellular carcinoma (HCC) with high epidemiological report data. Pathogenesis in HCC also involves several signaling pathways. This study aims to evaluate the in vitro activity of Moringa seed NanoChitosan against Hep G2 liver cancer cells and Co-Treatment with Doxorubicin. Initially, nanoparticles were prepared by extracting Moringa seeds, formulating them into nano chitosan, and then characterizing the compounds and particle sizes. The IC50 dose was investigated using the MTT assay. Then, the IC50 dose was confirmed in more detail through immunofluorescence, betatrophin gene, several genes in the Wnt-βcatenin-CyclinD1 proliferation pathway, and the addition of the apoptotic effector Caspase-3 using RT-qPCR analysis. Each treatment used a single dose of NCH-Mosee and co-treatment or combination with 4 μg/ml doxorubicin. The IC50 dose was 994 μg/ml in single treatment and 649 μg/ml in combined treatment with Dox. Hep G2 showed a decrease in the expression level of each parameter measured with increasing single dose and combination treatment (p < 0.050). Histologically, cells shrank, betatrophin expression was inhibited, and luminescence was seen, which decreased with increasing dose. In conclusion, NCH-Mosee with dose-tracking toxicity combined with Dox can suppress the viability of Hep G2 cells. https://journal.ipb.ac.id/index.php/hayati/article/view/58913 |
| spellingShingle | Hendra Susanto Moch Sholeh Agustina Tri Endharti Nik Ahmad Nizam Nik Malek Sa’diyatul Rizqie Amaliyah Firdaus Adeodatus Yuda Handaya Ahmad Taufiq Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer Cells Hayati Journal of Biosciences |
| title | Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer Cells |
| title_full | Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer Cells |
| title_fullStr | Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer Cells |
| title_full_unstemmed | Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer Cells |
| title_short | Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer Cells |
| title_sort | increased anti proliferation performance of nanochitosan moringa seeds extract and co treatment with doxorubicin in liver cancer cells |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/58913 |
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