Elimination of Ethanol for the Production of Fucoidans from Brown Seaweeds: Characterization and Bioactivities
Fucoidan, a sulphated polysaccharide from brown seaweed composed of several monosaccharides, has been stated to have several bioactive properties such as antioxidant, antiviral, anticancer, antithrombic, anti-inflammatory, and immunomodulatory effects. This paper provides research findings on green...
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2024-10-01
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| author | Periaswamy Sivagnanam Saravana Shanmugapriya Karuppusamy Dilip K. Rai Janith Wanigasekara James Curtin Brijesh K. Tiwari |
| author_facet | Periaswamy Sivagnanam Saravana Shanmugapriya Karuppusamy Dilip K. Rai Janith Wanigasekara James Curtin Brijesh K. Tiwari |
| author_sort | Periaswamy Sivagnanam Saravana |
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| description | Fucoidan, a sulphated polysaccharide from brown seaweed composed of several monosaccharides, has been stated to have several bioactive properties such as antioxidant, antiviral, anticancer, antithrombic, anti-inflammatory, and immunomodulatory effects. This paper provides research findings on green extraction methods, structural analysis of fucoidan, and its associated bioactivities. Fucoidans from brown seaweeds, <i>Fucus vesiculosus</i> and <i>Ascophyllum nodosum,</i> were extracted using green solvents such as citric acid (CA) followed by MWCO (molecular weight cut-off) filtration to obtain high-purity polysaccharides. The presence of functional groups typical to fucoidans, namely, fucose, sulfate, and glycosidic bonds, in the extracts were confirmed through the data obtained from FTIR (Fourier-transform infrared spectroscopy), TGA (thermogravimetric analysis), DSC (differential scanning calorimetry), and solid-state CP–MAS (cross-polarization magic angle spinning) analysis. The MWCO analysis identified that the >300 kDa fraction can have better content of fucoidan (FV-CA 79.16%, FV-HCl 63.59%, AN-CA 79.21%, AN-HCl 80.70%) than the conventional extraction process. Furthermore, the >300 kDa fraction showed significantly higher antioxidant activities compared to crude fucoidan extracts. Crude fucoidan extracts showed significant inhibition of cell viability in human lung (A459 lung carcinoma cells) and colorectal adenocarcinoma (Caco-2) cells at higher concentrations. The fucoidan extracted with green solvents and avoiding alcohol-based precipitation has substantial antioxidant/antitumor action, so, due to this activity, it can be employed as functional foods in food applications. |
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| institution | Kabale University |
| issn | 1660-3397 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Marine Drugs |
| spelling | doaj-art-cf87ced5e6a945858e8e393e66384a6a2024-11-26T18:11:20ZengMDPI AGMarine Drugs1660-33972024-10-01221149310.3390/md22110493Elimination of Ethanol for the Production of Fucoidans from Brown Seaweeds: Characterization and BioactivitiesPeriaswamy Sivagnanam Saravana0Shanmugapriya Karuppusamy1Dilip K. Rai2Janith Wanigasekara3James Curtin4Brijesh K. Tiwari5Department of Food Chemistry & Technology, Teagasc Food Research Centre, D15 DY05 Dublin, IrelandSchool of Biosystems and Food Engineering, University College Dublin, Belfield, D04 V1W8 Dublin, IrelandDepartment of Food Chemistry & Technology, Teagasc Food Research Centre, D15 DY05 Dublin, IrelandSchool of Food Science and Environmental Health, Technological University Dublin, D07 ADY7 Dublin, IrelandSchool of Food Science and Environmental Health, Technological University Dublin, D07 ADY7 Dublin, IrelandDepartment of Food Chemistry & Technology, Teagasc Food Research Centre, D15 DY05 Dublin, IrelandFucoidan, a sulphated polysaccharide from brown seaweed composed of several monosaccharides, has been stated to have several bioactive properties such as antioxidant, antiviral, anticancer, antithrombic, anti-inflammatory, and immunomodulatory effects. This paper provides research findings on green extraction methods, structural analysis of fucoidan, and its associated bioactivities. Fucoidans from brown seaweeds, <i>Fucus vesiculosus</i> and <i>Ascophyllum nodosum,</i> were extracted using green solvents such as citric acid (CA) followed by MWCO (molecular weight cut-off) filtration to obtain high-purity polysaccharides. The presence of functional groups typical to fucoidans, namely, fucose, sulfate, and glycosidic bonds, in the extracts were confirmed through the data obtained from FTIR (Fourier-transform infrared spectroscopy), TGA (thermogravimetric analysis), DSC (differential scanning calorimetry), and solid-state CP–MAS (cross-polarization magic angle spinning) analysis. The MWCO analysis identified that the >300 kDa fraction can have better content of fucoidan (FV-CA 79.16%, FV-HCl 63.59%, AN-CA 79.21%, AN-HCl 80.70%) than the conventional extraction process. Furthermore, the >300 kDa fraction showed significantly higher antioxidant activities compared to crude fucoidan extracts. Crude fucoidan extracts showed significant inhibition of cell viability in human lung (A459 lung carcinoma cells) and colorectal adenocarcinoma (Caco-2) cells at higher concentrations. The fucoidan extracted with green solvents and avoiding alcohol-based precipitation has substantial antioxidant/antitumor action, so, due to this activity, it can be employed as functional foods in food applications.https://www.mdpi.com/1660-3397/22/11/493seaweedsfucoidanmolecular weight cut offFTIRantioxidantcytotoxicity |
| spellingShingle | Periaswamy Sivagnanam Saravana Shanmugapriya Karuppusamy Dilip K. Rai Janith Wanigasekara James Curtin Brijesh K. Tiwari Elimination of Ethanol for the Production of Fucoidans from Brown Seaweeds: Characterization and Bioactivities Marine Drugs seaweeds fucoidan molecular weight cut off FTIR antioxidant cytotoxicity |
| title | Elimination of Ethanol for the Production of Fucoidans from Brown Seaweeds: Characterization and Bioactivities |
| title_full | Elimination of Ethanol for the Production of Fucoidans from Brown Seaweeds: Characterization and Bioactivities |
| title_fullStr | Elimination of Ethanol for the Production of Fucoidans from Brown Seaweeds: Characterization and Bioactivities |
| title_full_unstemmed | Elimination of Ethanol for the Production of Fucoidans from Brown Seaweeds: Characterization and Bioactivities |
| title_short | Elimination of Ethanol for the Production of Fucoidans from Brown Seaweeds: Characterization and Bioactivities |
| title_sort | elimination of ethanol for the production of fucoidans from brown seaweeds characterization and bioactivities |
| topic | seaweeds fucoidan molecular weight cut off FTIR antioxidant cytotoxicity |
| url | https://www.mdpi.com/1660-3397/22/11/493 |
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