Multiresistance to carbapenems by production of Verona integron-encoded metallo-β-lactamase (VIM) -type carbapenemases in Gram-negative bacilli isolated at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina Faso
Background: The production of metallo-beta-lactamases (MBL) such as VIM (Verona integron-encoded metallo-β-lactamase), IMP (Imipenem-resistant Pseudomonas) and NDM (New Delhi metallo-β-lactamase) in Gram-negative bacilli (GNB) resistant to carbapenems is a real concern for clinicians, given the ther...
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Zagazig University, Faculty of Medicine
2024-05-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_344552_05fd3c1ea79c3765875468eb3c242eb6.pdf |
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| author | Damis Yves Patrik BOUNIOUNOU Amana Metuor Dabiré Nicolas OUEDRAOGO Rahimatou Yasmine Tiemtore Serge Sougué Pegdwendé Rose Bonkoungou Jacques Simporé |
| author_facet | Damis Yves Patrik BOUNIOUNOU Amana Metuor Dabiré Nicolas OUEDRAOGO Rahimatou Yasmine Tiemtore Serge Sougué Pegdwendé Rose Bonkoungou Jacques Simporé |
| author_sort | Damis Yves Patrik BOUNIOUNOU |
| collection | DOAJ |
| description | Background: The production of metallo-beta-lactamases (MBL) such as VIM (Verona integron-encoded metallo-β-lactamase), IMP (Imipenem-resistant Pseudomonas) and NDM (New Delhi metallo-β-lactamase) in Gram-negative bacilli (GNB) resistant to carbapenems is a real concern for clinicians, given the therapeutic impasses involved. However, the existence of resistance genes encoding these enzymes is virtually undocumented in Burkina Faso. Aim: To genotypically demonstrate carbapenem resistance through the production of VIM-type carbapenemases in GNB strains collected at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina Faso. Methods: In this study, the resistance profile of 158 strains of GNB to imipenem, meropenem, ertapenem, doripenem and aztreonam was determined using the disc diffusion method. Resistant strains were analyzed by conventional PCR to detect blaVIM using specific primers. Results: Of 158 GNB strains collected, 91 (57.6%) were resistant to at least one of the carbapenems and/or aztreonam. The highest prevalence of resistant strains was observed in Escherichia coli (E. coli) 45.1% (n=41) and Klebsiella pneumoniae (K. pneumonia) 26.5% (n=24), which are the majority species. The blaVIM gene was detected in only 7 resistant strains (7.7%), including 3.3% (n=3/91) of E. coli, and 1.1% (n=1/91) of each of the species Pseudomonas aeruginosa (P. aeruginosa), Klebsiella oxytoca (K. oxytoca), Proteus mirabilis (P. mirabilis) and Serratia marcescens (S. marcescens). Conclusion: This study established the existence of blaVIM gene, which is involved in the resistance of GNB to carbapenems through the production of VIM-type enzymes at CHU-T and HOSCO in Burkina Faso. |
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| id | doaj-art-cf06691b9ed34db98a6ddf6731bec31d |
| institution | Kabale University |
| issn | 2682-4132 2682-4140 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Zagazig University, Faculty of Medicine |
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| spelling | doaj-art-cf06691b9ed34db98a6ddf6731bec31d2024-12-21T21:02:47ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-05-015276377310.21608/mid.2024.262512.1760344552Multiresistance to carbapenems by production of Verona integron-encoded metallo-β-lactamase (VIM) -type carbapenemases in Gram-negative bacilli isolated at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina FasoDamis Yves Patrik BOUNIOUNOU0Amana Metuor Dabiré1Nicolas OUEDRAOGO2Rahimatou Yasmine Tiemtore3Serge Sougué4Pegdwendé Rose Bonkoungou5Jacques Simporé6Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina FasoMolecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso National Center for Research and Training on Malaria (CNRFP), National Institute of Public Health (INSP), Burkina FasoMolecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina FasoMolecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso Saint Thomas Aquinas University, Ouagadougou Burkina FasoMolecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso Background: The production of metallo-beta-lactamases (MBL) such as VIM (Verona integron-encoded metallo-β-lactamase), IMP (Imipenem-resistant Pseudomonas) and NDM (New Delhi metallo-β-lactamase) in Gram-negative bacilli (GNB) resistant to carbapenems is a real concern for clinicians, given the therapeutic impasses involved. However, the existence of resistance genes encoding these enzymes is virtually undocumented in Burkina Faso. Aim: To genotypically demonstrate carbapenem resistance through the production of VIM-type carbapenemases in GNB strains collected at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina Faso. Methods: In this study, the resistance profile of 158 strains of GNB to imipenem, meropenem, ertapenem, doripenem and aztreonam was determined using the disc diffusion method. Resistant strains were analyzed by conventional PCR to detect blaVIM using specific primers. Results: Of 158 GNB strains collected, 91 (57.6%) were resistant to at least one of the carbapenems and/or aztreonam. The highest prevalence of resistant strains was observed in Escherichia coli (E. coli) 45.1% (n=41) and Klebsiella pneumoniae (K. pneumonia) 26.5% (n=24), which are the majority species. The blaVIM gene was detected in only 7 resistant strains (7.7%), including 3.3% (n=3/91) of E. coli, and 1.1% (n=1/91) of each of the species Pseudomonas aeruginosa (P. aeruginosa), Klebsiella oxytoca (K. oxytoca), Proteus mirabilis (P. mirabilis) and Serratia marcescens (S. marcescens). Conclusion: This study established the existence of blaVIM gene, which is involved in the resistance of GNB to carbapenems through the production of VIM-type enzymes at CHU-T and HOSCO in Burkina Faso.https://mid.journals.ekb.eg/article_344552_05fd3c1ea79c3765875468eb3c242eb6.pdfcarbapenem resistancecarbapenemaseblavimgram-negative bacilliburkina faso |
| spellingShingle | Damis Yves Patrik BOUNIOUNOU Amana Metuor Dabiré Nicolas OUEDRAOGO Rahimatou Yasmine Tiemtore Serge Sougué Pegdwendé Rose Bonkoungou Jacques Simporé Multiresistance to carbapenems by production of Verona integron-encoded metallo-β-lactamase (VIM) -type carbapenemases in Gram-negative bacilli isolated at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina Faso Microbes and Infectious Diseases carbapenem resistance carbapenemase blavim gram-negative bacilli burkina faso |
| title | Multiresistance to carbapenems by production of Verona integron-encoded metallo-β-lactamase (VIM) -type carbapenemases in Gram-negative bacilli isolated at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina Faso |
| title_full | Multiresistance to carbapenems by production of Verona integron-encoded metallo-β-lactamase (VIM) -type carbapenemases in Gram-negative bacilli isolated at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina Faso |
| title_fullStr | Multiresistance to carbapenems by production of Verona integron-encoded metallo-β-lactamase (VIM) -type carbapenemases in Gram-negative bacilli isolated at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina Faso |
| title_full_unstemmed | Multiresistance to carbapenems by production of Verona integron-encoded metallo-β-lactamase (VIM) -type carbapenemases in Gram-negative bacilli isolated at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina Faso |
| title_short | Multiresistance to carbapenems by production of Verona integron-encoded metallo-β-lactamase (VIM) -type carbapenemases in Gram-negative bacilli isolated at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina Faso |
| title_sort | multiresistance to carbapenems by production of verona integron encoded metallo β lactamase vim type carbapenemases in gram negative bacilli isolated at the centre hospitalier universitaire de tengandogo chu t and the hopital saint camille de ouagadougou hosco in burkina faso |
| topic | carbapenem resistance carbapenemase blavim gram-negative bacilli burkina faso |
| url | https://mid.journals.ekb.eg/article_344552_05fd3c1ea79c3765875468eb3c242eb6.pdf |
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