Strategy of employing plug-and-play vectors and LC–MS screening to facilitate the discovery of natural products using Aspergillus oryzae

Strategy of employing plug-and-play vectors and LC–MS screening to facilitate the discovery of natural products using Aspergillus oryzae

Abstract Aspergillus oryzae is a widely used host for heterologous expression of fungal natural products. However, the vectors previously developed are not convenient for use and screening positive transformants by PCR and fermentation is time- and effort-consuming. Hence, three plug-and-play vector...

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Bibliographic Details
Main Authors: Hanliang Shi, Beibei Lin, Mengmeng Zheng, Fengyu Gan, Zhi Lin, Xiujuan Xin, Jian Zhao, Xudong Qu, Faliang An
Format: Article
Language:English
Published: SpringerOpen 2025-01-01
Series:Bioresources and Bioprocessing
Subjects:
Plug-and-play vectors
LC–MS detection
Aspergillus oryzae
Heterologous expression
PamyB
Online Access:https://doi.org/10.1186/s40643-024-00833-w
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