Ecological safety of insecticide based on entomopathogenic virus DsCPV-1 for nontarget invertebrates
Abstract For the first time under laboratory conditions, the virulence of a unique cypovirus strain, DsCPV-1, which has broad host specificity, was tested on nontarget aquatic organisms (natural species: Gammarus lacustris, Anopheles messeae, Coenagrion lunulatum, Cloeon robusta, Chironomus sp., Ily...
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Nature Portfolio
2024-11-01
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| Online Access: | https://doi.org/10.1038/s41598-024-78471-7 |
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| author | Olga Belevitch Yuri Yurchenko Daria Kharlamova Elena Shatalova Natalia Agrikolyanskaya Anna Subbotina Anastasia Ignatieva Yuri Tokarev Vyacheslav Martemyanov |
| author_facet | Olga Belevitch Yuri Yurchenko Daria Kharlamova Elena Shatalova Natalia Agrikolyanskaya Anna Subbotina Anastasia Ignatieva Yuri Tokarev Vyacheslav Martemyanov |
| author_sort | Olga Belevitch |
| collection | DOAJ |
| description | Abstract For the first time under laboratory conditions, the virulence of a unique cypovirus strain, DsCPV-1, which has broad host specificity, was tested on nontarget aquatic organisms (natural species: Gammarus lacustris, Anopheles messeae, Coenagrion lunulatum, Cloeon robusta, Chironomus sp., Ilyocoris cimicoides, and Plea minutissima; laboratory species: Aedes aegypti and Daphnia magna), a terrestrial pollinator species (Apis mellifera), and an entomophage (Podisus maculiventris). The probability of this virus’s accumulation in the bodies of invertebrates and of its transmission along a trophic chain was evaluated by two approaches: bioassays and a molecular diagnostic analysis. In the bioassays, there was no significant increase in mortality among all the tested aquatic and terrestrial nontarget species exposed to DsCPV-1 as compared with control groups (no virus). When we fed Podisus maculiventris with caterpillars having active DsCPV-1 infection (i.e., with the virus replicating in the host) no viral replication was observed in bug. No replication was also observed in mosquitos as well as in bee after viral treatment. Thus, the results show that the DsCPV-1 virus has excellent environmental safety toward many invertebrate species and can be recommended for the control of lepidopteran pests in forestry and agriculture as insecticide with light effect on environment. |
| format | Article |
| id | doaj-art-ced3bd80cac7416bbc4dba13a40b6aff |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-ced3bd80cac7416bbc4dba13a40b6aff2024-11-24T12:24:21ZengNature PortfolioScientific Reports2045-23222024-11-0114111010.1038/s41598-024-78471-7Ecological safety of insecticide based on entomopathogenic virus DsCPV-1 for nontarget invertebratesOlga Belevitch0Yuri Yurchenko1Daria Kharlamova2Elena Shatalova3Natalia Agrikolyanskaya4Anna Subbotina5Anastasia Ignatieva6Yuri Tokarev7Vyacheslav Martemyanov8Institute of Systematics and Ecology of Animals SB RASInstitute of Systematics and Ecology of Animals SB RASInstitute of Systematics and Ecology of Animals SB RASSiberian Federal Scientific Centre of Agrobiotechnologies of the Russian Academy of Sciences (SFSCA RAS)Siberian Federal Scientific Centre of Agrobiotechnologies of the Russian Academy of Sciences (SFSCA RAS)Institute of Systematics and Ecology of Animals SB RASAll-Russian Institute of Plant ProtectionAll-Russian Institute of Plant ProtectionInstitute of Systematics and Ecology of Animals SB RASAbstract For the first time under laboratory conditions, the virulence of a unique cypovirus strain, DsCPV-1, which has broad host specificity, was tested on nontarget aquatic organisms (natural species: Gammarus lacustris, Anopheles messeae, Coenagrion lunulatum, Cloeon robusta, Chironomus sp., Ilyocoris cimicoides, and Plea minutissima; laboratory species: Aedes aegypti and Daphnia magna), a terrestrial pollinator species (Apis mellifera), and an entomophage (Podisus maculiventris). The probability of this virus’s accumulation in the bodies of invertebrates and of its transmission along a trophic chain was evaluated by two approaches: bioassays and a molecular diagnostic analysis. In the bioassays, there was no significant increase in mortality among all the tested aquatic and terrestrial nontarget species exposed to DsCPV-1 as compared with control groups (no virus). When we fed Podisus maculiventris with caterpillars having active DsCPV-1 infection (i.e., with the virus replicating in the host) no viral replication was observed in bug. No replication was also observed in mosquitos as well as in bee after viral treatment. Thus, the results show that the DsCPV-1 virus has excellent environmental safety toward many invertebrate species and can be recommended for the control of lepidopteran pests in forestry and agriculture as insecticide with light effect on environment.https://doi.org/10.1038/s41598-024-78471-7HydrobiontPollinatorEntomophageHoney beeEcologically friendly bioinsecticideGreen technology |
| spellingShingle | Olga Belevitch Yuri Yurchenko Daria Kharlamova Elena Shatalova Natalia Agrikolyanskaya Anna Subbotina Anastasia Ignatieva Yuri Tokarev Vyacheslav Martemyanov Ecological safety of insecticide based on entomopathogenic virus DsCPV-1 for nontarget invertebrates Scientific Reports Hydrobiont Pollinator Entomophage Honey bee Ecologically friendly bioinsecticide Green technology |
| title | Ecological safety of insecticide based on entomopathogenic virus DsCPV-1 for nontarget invertebrates |
| title_full | Ecological safety of insecticide based on entomopathogenic virus DsCPV-1 for nontarget invertebrates |
| title_fullStr | Ecological safety of insecticide based on entomopathogenic virus DsCPV-1 for nontarget invertebrates |
| title_full_unstemmed | Ecological safety of insecticide based on entomopathogenic virus DsCPV-1 for nontarget invertebrates |
| title_short | Ecological safety of insecticide based on entomopathogenic virus DsCPV-1 for nontarget invertebrates |
| title_sort | ecological safety of insecticide based on entomopathogenic virus dscpv 1 for nontarget invertebrates |
| topic | Hydrobiont Pollinator Entomophage Honey bee Ecologically friendly bioinsecticide Green technology |
| url | https://doi.org/10.1038/s41598-024-78471-7 |
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