The oncogenic roles of GPR176 in ovarian cancer: a molecular target for aggressiveness and gene therapy
Background At present, the discovery of new biomarkers is of great significance for the early diagnosis, treatment and prognosis assessment of ovarian cancer. Previous findings indicated that aberrant G-protein-coupled receptor 176 (GPR176) expression might contribute to tumorigenesis and subsequent...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Obstetrics and Gynaecology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/01443615.2024.2347430 |
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| author | Ning Yang Wen-jing Yun Zheng-guo Cui Hua-chuan Zheng |
| author_facet | Ning Yang Wen-jing Yun Zheng-guo Cui Hua-chuan Zheng |
| author_sort | Ning Yang |
| collection | DOAJ |
| description | Background At present, the discovery of new biomarkers is of great significance for the early diagnosis, treatment and prognosis assessment of ovarian cancer. Previous findings indicated that aberrant G-protein-coupled receptor 176 (GPR176) expression might contribute to tumorigenesis and subsequent progression. However, the expression of GPR176 and the molecular mechanisms in ovarian cancer had not been investigated.Methods GPR176 expression was compared with clinicopathological features of ovarian cancer using immunohistochemical and bioinformatics analyses. GPR176-related genes and pathways were analysed using bioinformatics analysis. Additionally, the effects of GPR176 on ovarian cancer cell phenotypes were investigated.Results GPR176 expression positively correlated with elder age, clinicopathological staging, tumour residual status, and unfavourable survival of ovarian cancer, but negatively with purity loss, infiltration of B cells, and CD8+ T cells. Gene Set Enrichment Analysis showed that differential expression of GPR176 was involved in focal adhesion, ECM-receptor interaction, cell adhesion molecules and so on. STRING and Cytoscape were used to determine the top 10 nodes. Kyoto Encyclopaedia of Genes and Genomes analysis indicated that GPR176-related genes were involved in the ECM structural constituent and organisation and so on. GPR176 overexpression promoted the proliferation, anti-apoptosis, anti-pyroptosis, migration and invasion of ovarian cancer cells with overexpression of N-cadherin, Zeb1, Snail, Twist1, and under-expression of gasdermin D, caspase 1, and E-cadherin.Conclusion GPR176 might be involved in the progression of ovarian cancer. It might be used as a biomarker to indicate the aggressive behaviour and poor prognosis of ovarian cancer and a target of genetic therapy. |
| format | Article |
| id | doaj-art-ce23fe178a60478f8443b150aa0f622d |
| institution | Kabale University |
| issn | 0144-3615 1364-6893 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Obstetrics and Gynaecology |
| spelling | doaj-art-ce23fe178a60478f8443b150aa0f622d2025-01-09T12:13:17ZengTaylor & Francis GroupJournal of Obstetrics and Gynaecology0144-36151364-68932024-12-0144110.1080/01443615.2024.2347430The oncogenic roles of GPR176 in ovarian cancer: a molecular target for aggressiveness and gene therapyNing Yang0Wen-jing Yun1Zheng-guo Cui2Hua-chuan Zheng3Department of Oncology and Central Laboratory, The Affiliated Hospital of Chengde Medical University, Chengde, ChinaDepartment of Oncology and Central Laboratory, The Affiliated Hospital of Chengde Medical University, Chengde, ChinaDepartment of Environmental Health, University of Fukui School of Medical Science, Fukui, JapanDepartment of Oncology and Central Laboratory, The Affiliated Hospital of Chengde Medical University, Chengde, ChinaBackground At present, the discovery of new biomarkers is of great significance for the early diagnosis, treatment and prognosis assessment of ovarian cancer. Previous findings indicated that aberrant G-protein-coupled receptor 176 (GPR176) expression might contribute to tumorigenesis and subsequent progression. However, the expression of GPR176 and the molecular mechanisms in ovarian cancer had not been investigated.Methods GPR176 expression was compared with clinicopathological features of ovarian cancer using immunohistochemical and bioinformatics analyses. GPR176-related genes and pathways were analysed using bioinformatics analysis. Additionally, the effects of GPR176 on ovarian cancer cell phenotypes were investigated.Results GPR176 expression positively correlated with elder age, clinicopathological staging, tumour residual status, and unfavourable survival of ovarian cancer, but negatively with purity loss, infiltration of B cells, and CD8+ T cells. Gene Set Enrichment Analysis showed that differential expression of GPR176 was involved in focal adhesion, ECM-receptor interaction, cell adhesion molecules and so on. STRING and Cytoscape were used to determine the top 10 nodes. Kyoto Encyclopaedia of Genes and Genomes analysis indicated that GPR176-related genes were involved in the ECM structural constituent and organisation and so on. GPR176 overexpression promoted the proliferation, anti-apoptosis, anti-pyroptosis, migration and invasion of ovarian cancer cells with overexpression of N-cadherin, Zeb1, Snail, Twist1, and under-expression of gasdermin D, caspase 1, and E-cadherin.Conclusion GPR176 might be involved in the progression of ovarian cancer. It might be used as a biomarker to indicate the aggressive behaviour and poor prognosis of ovarian cancer and a target of genetic therapy.https://www.tandfonline.com/doi/10.1080/01443615.2024.2347430GPR176Ovarian cancer |
| spellingShingle | Ning Yang Wen-jing Yun Zheng-guo Cui Hua-chuan Zheng The oncogenic roles of GPR176 in ovarian cancer: a molecular target for aggressiveness and gene therapy Journal of Obstetrics and Gynaecology GPR176 Ovarian cancer |
| title | The oncogenic roles of GPR176 in ovarian cancer: a molecular target for aggressiveness and gene therapy |
| title_full | The oncogenic roles of GPR176 in ovarian cancer: a molecular target for aggressiveness and gene therapy |
| title_fullStr | The oncogenic roles of GPR176 in ovarian cancer: a molecular target for aggressiveness and gene therapy |
| title_full_unstemmed | The oncogenic roles of GPR176 in ovarian cancer: a molecular target for aggressiveness and gene therapy |
| title_short | The oncogenic roles of GPR176 in ovarian cancer: a molecular target for aggressiveness and gene therapy |
| title_sort | oncogenic roles of gpr176 in ovarian cancer a molecular target for aggressiveness and gene therapy |
| topic | GPR176 Ovarian cancer |
| url | https://www.tandfonline.com/doi/10.1080/01443615.2024.2347430 |
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