Targeted K‐Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers using Spectral Photon Counting CT
Abstract Utilizing metal nanoprobes with unique K‐edge identities to visualize complementary biological activities simultaneously can provide valuable information about complex biological processes. This study describes the design and preparation of an innovative pair of K‐edge metal nanoprobes and...
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| Format: | Article |
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Wiley
2024-12-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202408408 |
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| author | Nivetha Gunaseelan Parikshit Moitra Pranay Saha Teresa Aditya Mahdieh Moghiseh Kevin Jonker Steven Gieseg Anthony Butler Fadia Kamal Dipanjan Pan |
| author_facet | Nivetha Gunaseelan Parikshit Moitra Pranay Saha Teresa Aditya Mahdieh Moghiseh Kevin Jonker Steven Gieseg Anthony Butler Fadia Kamal Dipanjan Pan |
| author_sort | Nivetha Gunaseelan |
| collection | DOAJ |
| description | Abstract Utilizing metal nanoprobes with unique K‐edge identities to visualize complementary biological activities simultaneously can provide valuable information about complex biological processes. This study describes the design and preparation of an innovative pair of K‐edge metal nanoprobes and demonstrates the feasibility of their simultaneous quantitative detection using spectral photon‐counting computed tomography (SPCCT). Glycosaminoglycan (GAG) capped nanoparticles (ca. 15–20 nm) targeting two distinct components of the cartilage tissue, namely, aggrecan (acan) and aggrecanase (acanase) are designed and synthesized. These targeted nanoparticles comprised of praseodymium (Pr) and hafnium (Hf), with well‐separated K‐edge energies, enable simultaneous and ratiometric imaging of dual biomarkers in cartilage tissue. Following extensive physico‐chemical characterization of the ligand‐targeted particles, the feasibility of homing dual biomarkers in vitro is demonstrated. The material discrimination and simultaneous quantification of these targeted particles are also achieved and corroborated with inductively coupled plasmon spectroscopy. For the first time, the use of praseodymium is reported as a contrast agent for SPCCT imaging and demonstrates the ability to pair it with hafnium nanoprobes for multicontrast imaging of diseases. Importantly, the potential for ratiometric molecular imaging and tracking of osteoarthritis (OA) progression is shown with SPCCT K‐edge based imaging approach. |
| format | Article |
| id | doaj-art-ce019b15b0a447a8889d243f3cb508ab |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-ce019b15b0a447a8889d243f3cb508ab2024-12-11T16:00:49ZengWileyAdvanced Science2198-38442024-12-011146n/an/a10.1002/advs.202408408Targeted K‐Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers using Spectral Photon Counting CTNivetha Gunaseelan0Parikshit Moitra1Pranay Saha2Teresa Aditya3Mahdieh Moghiseh4Kevin Jonker5Steven Gieseg6Anthony Butler7Fadia Kamal8Dipanjan Pan9Huck Institutes of the Life Sciences Department(s) of Biomedical Engineering Nuclear Engineering Materials Science and Engineering The Pennsylvania State University 101 Huck Life Sciences Building University Park PA 16802 USAHuck Institutes of the Life Sciences Department(s) of Biomedical Engineering Nuclear Engineering Materials Science and Engineering The Pennsylvania State University 101 Huck Life Sciences Building University Park PA 16802 USAHuck Institutes of the Life Sciences Department(s) of Biomedical Engineering Nuclear Engineering Materials Science and Engineering The Pennsylvania State University 101 Huck Life Sciences Building University Park PA 16802 USAHuck Institutes of the Life Sciences Department(s) of Biomedical Engineering Nuclear Engineering Materials Science and Engineering The Pennsylvania State University 101 Huck Life Sciences Building University Park PA 16802 USAMARS Bioimaging Limited 68 Saint Asaph Street, Christchurch Central City Christchurch 8011 New ZealandMARS Bioimaging Limited 68 Saint Asaph Street, Christchurch Central City Christchurch 8011 New ZealandMARS Bioimaging Limited 68 Saint Asaph Street, Christchurch Central City Christchurch 8011 New ZealandMARS Bioimaging Limited 68 Saint Asaph Street, Christchurch Central City Christchurch 8011 New ZealandCenter for Orthopaedic Research and Translational Science Department of Orthopaedics and Rehabilitation Penn State College of Medicine The Pennsylvania State University Hershey PA 17033 USAHuck Institutes of the Life Sciences Department(s) of Biomedical Engineering Nuclear Engineering Materials Science and Engineering The Pennsylvania State University 101 Huck Life Sciences Building University Park PA 16802 USAAbstract Utilizing metal nanoprobes with unique K‐edge identities to visualize complementary biological activities simultaneously can provide valuable information about complex biological processes. This study describes the design and preparation of an innovative pair of K‐edge metal nanoprobes and demonstrates the feasibility of their simultaneous quantitative detection using spectral photon‐counting computed tomography (SPCCT). Glycosaminoglycan (GAG) capped nanoparticles (ca. 15–20 nm) targeting two distinct components of the cartilage tissue, namely, aggrecan (acan) and aggrecanase (acanase) are designed and synthesized. These targeted nanoparticles comprised of praseodymium (Pr) and hafnium (Hf), with well‐separated K‐edge energies, enable simultaneous and ratiometric imaging of dual biomarkers in cartilage tissue. Following extensive physico‐chemical characterization of the ligand‐targeted particles, the feasibility of homing dual biomarkers in vitro is demonstrated. The material discrimination and simultaneous quantification of these targeted particles are also achieved and corroborated with inductively coupled plasmon spectroscopy. For the first time, the use of praseodymium is reported as a contrast agent for SPCCT imaging and demonstrates the ability to pair it with hafnium nanoprobes for multicontrast imaging of diseases. Importantly, the potential for ratiometric molecular imaging and tracking of osteoarthritis (OA) progression is shown with SPCCT K‐edge based imaging approach.https://doi.org/10.1002/advs.202408408hafnium nanoparticlesK‐edge imagingosteoarthritis trackingpraseodymium nanoparticlesquantitative imagingspectral photon counting computed tomography |
| spellingShingle | Nivetha Gunaseelan Parikshit Moitra Pranay Saha Teresa Aditya Mahdieh Moghiseh Kevin Jonker Steven Gieseg Anthony Butler Fadia Kamal Dipanjan Pan Targeted K‐Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers using Spectral Photon Counting CT Advanced Science hafnium nanoparticles K‐edge imaging osteoarthritis tracking praseodymium nanoparticles quantitative imaging spectral photon counting computed tomography |
| title | Targeted K‐Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers using Spectral Photon Counting CT |
| title_full | Targeted K‐Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers using Spectral Photon Counting CT |
| title_fullStr | Targeted K‐Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers using Spectral Photon Counting CT |
| title_full_unstemmed | Targeted K‐Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers using Spectral Photon Counting CT |
| title_short | Targeted K‐Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers using Spectral Photon Counting CT |
| title_sort | targeted k edge nanoprobes from praseodymium and hafnium for ratiometric tracking of dual biomarkers using spectral photon counting ct |
| topic | hafnium nanoparticles K‐edge imaging osteoarthritis tracking praseodymium nanoparticles quantitative imaging spectral photon counting computed tomography |
| url | https://doi.org/10.1002/advs.202408408 |
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