Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy
Adeno-associated virus (AAV)-based gene therapy has become one of the key directions of modern translational medicine geared towards treatment of hereditary disorders by means of gene replacement. At the moment, about 5,000 different syndromes are associated with mutations in large genes, which pres...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1488912/full |
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| author | Andrew Brovin Ekaterina Minskaia Matvei Sabantsev Sergey Chuvpilo Alexander Karabelsky |
| author_facet | Andrew Brovin Ekaterina Minskaia Matvei Sabantsev Sergey Chuvpilo Alexander Karabelsky |
| author_sort | Andrew Brovin |
| collection | DOAJ |
| description | Adeno-associated virus (AAV)-based gene therapy has become one of the key directions of modern translational medicine geared towards treatment of hereditary disorders by means of gene replacement. At the moment, about 5,000 different syndromes are associated with mutations in large genes, which presents a great problem due to the AAV packaging capacity of 5 kilobases. The main strategies for overcoming this obstacle were the creation of truncated gene versions, overloading the viral vector, and separate delivery of partial genetic material to restore the whole gene at the level of DNA, RNA, or protein. At present, genome editing via prime editors, most effectively delivered by AAV, relies on the intein pair used to restore the protein complex. The amazing integration speed of intein-based protein trans splicing technology makes it a versatile tool for a variety of applications, albeit not always successful on the first attempt. This study discusses the key points of working with Ssp, Npu, and Ava inteins of the DnaE group, known as the most effective for assembly of large proteins. Using green fluorescent protein (GFP) as a model, we demonstrate that the successful protein assembly requires not only cysteine at position C+1 but also certain aminoacid residues on either side in its immediate environment. Furthermore, the conformation of extein-intein composition, difficult to predict by computer modeling, has an additional effect, as demonstrated by experimental tests of the three split sites optimal in amino acid composition. The NpuDnaE variant demonstrated the highest kinetics of interaction between the N and C parts in the DnaE group of inteins. Optimization of conditions using NpuDnaE intein led to GFP assembly in 80% of transfected HEK293 cells and in 55% of AAV5-transduced cells, as demonstrated by flow cytometry. The efficiency of GFP assembly post-plasmid DNA transfection or AAV transduction of the HEK293 cell line was 15% higher than that of the ARPE19 cell line. We hope that the obtained data will facilitate the development of gene therapies for the treatment of hereditary disorders caused by mutations in large genes. |
| format | Article |
| id | doaj-art-cdc7908a591e4760b6e2bbdf7e93c4fd |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-cdc7908a591e4760b6e2bbdf7e93c4fd2024-11-20T04:48:13ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-11-011210.3389/fbioe.2024.14889121488912Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapyAndrew BrovinEkaterina MinskaiaMatvei SabantsevSergey ChuvpiloAlexander KarabelskyAdeno-associated virus (AAV)-based gene therapy has become one of the key directions of modern translational medicine geared towards treatment of hereditary disorders by means of gene replacement. At the moment, about 5,000 different syndromes are associated with mutations in large genes, which presents a great problem due to the AAV packaging capacity of 5 kilobases. The main strategies for overcoming this obstacle were the creation of truncated gene versions, overloading the viral vector, and separate delivery of partial genetic material to restore the whole gene at the level of DNA, RNA, or protein. At present, genome editing via prime editors, most effectively delivered by AAV, relies on the intein pair used to restore the protein complex. The amazing integration speed of intein-based protein trans splicing technology makes it a versatile tool for a variety of applications, albeit not always successful on the first attempt. This study discusses the key points of working with Ssp, Npu, and Ava inteins of the DnaE group, known as the most effective for assembly of large proteins. Using green fluorescent protein (GFP) as a model, we demonstrate that the successful protein assembly requires not only cysteine at position C+1 but also certain aminoacid residues on either side in its immediate environment. Furthermore, the conformation of extein-intein composition, difficult to predict by computer modeling, has an additional effect, as demonstrated by experimental tests of the three split sites optimal in amino acid composition. The NpuDnaE variant demonstrated the highest kinetics of interaction between the N and C parts in the DnaE group of inteins. Optimization of conditions using NpuDnaE intein led to GFP assembly in 80% of transfected HEK293 cells and in 55% of AAV5-transduced cells, as demonstrated by flow cytometry. The efficiency of GFP assembly post-plasmid DNA transfection or AAV transduction of the HEK293 cell line was 15% higher than that of the ARPE19 cell line. We hope that the obtained data will facilitate the development of gene therapies for the treatment of hereditary disorders caused by mutations in large genes.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1488912/fullgene therapyAAVGFPinteinsprotein trans-splicing |
| spellingShingle | Andrew Brovin Ekaterina Minskaia Matvei Sabantsev Sergey Chuvpilo Alexander Karabelsky Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy Frontiers in Bioengineering and Biotechnology gene therapy AAV GFP inteins protein trans-splicing |
| title | Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy |
| title_full | Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy |
| title_fullStr | Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy |
| title_full_unstemmed | Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy |
| title_short | Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy |
| title_sort | protein trans splicing optimization of intein mediated gfp assembly as a model for the development of gene therapy |
| topic | gene therapy AAV GFP inteins protein trans-splicing |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1488912/full |
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