Proteomic profiling of neonatal herpes simplex virus infection on dried blood spots
Abstract Background: Neonatal herpes simplex virus (HSV) infection is life-threatening, with a mortality of up to 70–80% when disseminated, often due to vague symptoms and delayed treatment. Neonatal screening using dried blood spot (DBS) samples is among the most impactful preventative health measu...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Communications Medicine |
| Online Access: | https://doi.org/10.1038/s43856-024-00711-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846112300659900416 |
|---|---|
| author | Kia Hee Schultz Dungu Christian Munch Hagen Marie Bækvad-Hansen Victor Yakimov Alfonso Buil Demur Emma Malchau Carlsen Nadja Hawwa Vissing Tine Brink Henriksen Trine Hyrup Mogensen David Michael Hougaard Ulrikka Nygaard Jonas Bybjerg-Grauholm |
| author_facet | Kia Hee Schultz Dungu Christian Munch Hagen Marie Bækvad-Hansen Victor Yakimov Alfonso Buil Demur Emma Malchau Carlsen Nadja Hawwa Vissing Tine Brink Henriksen Trine Hyrup Mogensen David Michael Hougaard Ulrikka Nygaard Jonas Bybjerg-Grauholm |
| author_sort | Kia Hee Schultz Dungu |
| collection | DOAJ |
| description | Abstract Background: Neonatal herpes simplex virus (HSV) infection is life-threatening, with a mortality of up to 70–80% when disseminated, often due to vague symptoms and delayed treatment. Neonatal screening using dried blood spot (DBS) samples is among the most impactful preventative health measures ever implemented, but screening for HSV has not been investigated. Methods: We investigated high throughput multiplexed proteomics on DBS samples collected on days 2–3 of life from a nationwide cohort of neonates with HSV infection (n = 53) and matched controls. We measured 2941 proteins using the Olink Explore 3072 panels and proximity extension assays, followed by differential protein expression by Analysis of Variance with post-hoc correction and functional annotation. Results: Here, we show distinct protein profiles in neonates with disseminated HSV disease, with differences in 20 proteins compared to controls. These proteins are associated with innate and adaptive immune responses and cytokine activation. Conclusions: Our findings indicate the potential of neonatal screening for disseminated HSV disease to ensure early treatment and reduce the high mortality. |
| format | Article |
| id | doaj-art-cdc60cf2c66442f4a8475c7364f1ac27 |
| institution | Kabale University |
| issn | 2730-664X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Medicine |
| spelling | doaj-art-cdc60cf2c66442f4a8475c7364f1ac272024-12-22T12:44:06ZengNature PortfolioCommunications Medicine2730-664X2024-12-01411810.1038/s43856-024-00711-8Proteomic profiling of neonatal herpes simplex virus infection on dried blood spotsKia Hee Schultz Dungu0Christian Munch Hagen1Marie Bækvad-Hansen2Victor Yakimov3Alfonso Buil Demur4Emma Malchau Carlsen5Nadja Hawwa Vissing6Tine Brink Henriksen7Trine Hyrup Mogensen8David Michael Hougaard9Ulrikka Nygaard10Jonas Bybjerg-Grauholm11Department of Paediatrics and Adolescent Medicine, Copenhagen University HospitalDepartment for Congenital Disorders, Statens Serum InstitutDepartment for Congenital Disorders, Statens Serum InstitutDepartment for Congenital Disorders, Statens Serum InstitutMental Health Centre Sct. Hans, Capital Region of Denmark, Institute of Biological Psychiatry, Copenhagen University HospitalDepartment of Clinical Medicine, University of CopenhagenDepartment of Paediatrics and Adolescent Medicine, Copenhagen University HospitalDepartment of Paediatrics & Adolescent Medicine, Aarhus University HospitalDepartment of Infectious Diseases, Aarhus University HospitalDepartment for Congenital Disorders, Statens Serum InstitutDepartment of Paediatrics and Adolescent Medicine, Copenhagen University HospitalDepartment for Congenital Disorders, Statens Serum InstitutAbstract Background: Neonatal herpes simplex virus (HSV) infection is life-threatening, with a mortality of up to 70–80% when disseminated, often due to vague symptoms and delayed treatment. Neonatal screening using dried blood spot (DBS) samples is among the most impactful preventative health measures ever implemented, but screening for HSV has not been investigated. Methods: We investigated high throughput multiplexed proteomics on DBS samples collected on days 2–3 of life from a nationwide cohort of neonates with HSV infection (n = 53) and matched controls. We measured 2941 proteins using the Olink Explore 3072 panels and proximity extension assays, followed by differential protein expression by Analysis of Variance with post-hoc correction and functional annotation. Results: Here, we show distinct protein profiles in neonates with disseminated HSV disease, with differences in 20 proteins compared to controls. These proteins are associated with innate and adaptive immune responses and cytokine activation. Conclusions: Our findings indicate the potential of neonatal screening for disseminated HSV disease to ensure early treatment and reduce the high mortality.https://doi.org/10.1038/s43856-024-00711-8 |
| spellingShingle | Kia Hee Schultz Dungu Christian Munch Hagen Marie Bækvad-Hansen Victor Yakimov Alfonso Buil Demur Emma Malchau Carlsen Nadja Hawwa Vissing Tine Brink Henriksen Trine Hyrup Mogensen David Michael Hougaard Ulrikka Nygaard Jonas Bybjerg-Grauholm Proteomic profiling of neonatal herpes simplex virus infection on dried blood spots Communications Medicine |
| title | Proteomic profiling of neonatal herpes simplex virus infection on dried blood spots |
| title_full | Proteomic profiling of neonatal herpes simplex virus infection on dried blood spots |
| title_fullStr | Proteomic profiling of neonatal herpes simplex virus infection on dried blood spots |
| title_full_unstemmed | Proteomic profiling of neonatal herpes simplex virus infection on dried blood spots |
| title_short | Proteomic profiling of neonatal herpes simplex virus infection on dried blood spots |
| title_sort | proteomic profiling of neonatal herpes simplex virus infection on dried blood spots |
| url | https://doi.org/10.1038/s43856-024-00711-8 |
| work_keys_str_mv | AT kiaheeschultzdungu proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT christianmunchhagen proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT mariebækvadhansen proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT victoryakimov proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT alfonsobuildemur proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT emmamalchaucarlsen proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT nadjahawwavissing proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT tinebrinkhenriksen proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT trinehyrupmogensen proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT davidmichaelhougaard proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT ulrikkanygaard proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots AT jonasbybjerggrauholm proteomicprofilingofneonatalherpessimplexvirusinfectionondriedbloodspots |