Biochemical and psychological markers of fatigue and recovery in mixed martial arts athletes during strength and conditioning training

Abstract Mixed martial arts (MMA) training imposes significant physiological and psychological demands, increasing the risk of overtraining. This study aimed to assess temporal changes in biochemical and psychological fatigue and recovery markers during a structured 3-week strength and conditioning...

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Main Authors: Joanna Ostapiuk-Karolczuk, Hanna Dziewiecka, Patryk Bojsa, Mirosława Cieślicka, Monika Zawadka-Kunikowska, Kaźmierczak Wojciech, Anna Kasperska
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-09719-z
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author Joanna Ostapiuk-Karolczuk
Hanna Dziewiecka
Patryk Bojsa
Mirosława Cieślicka
Monika Zawadka-Kunikowska
Kaźmierczak Wojciech
Anna Kasperska
author_facet Joanna Ostapiuk-Karolczuk
Hanna Dziewiecka
Patryk Bojsa
Mirosława Cieślicka
Monika Zawadka-Kunikowska
Kaźmierczak Wojciech
Anna Kasperska
author_sort Joanna Ostapiuk-Karolczuk
collection DOAJ
description Abstract Mixed martial arts (MMA) training imposes significant physiological and psychological demands, increasing the risk of overtraining. This study aimed to assess temporal changes in biochemical and psychological fatigue and recovery markers during a structured 3-week strength and conditioning training program in professional MMA athletes. Ten male MMA athletes (26.2 ± 0.9 years; 7.0 ± 2.0 years of training experience) participated. Blood samples and psychological assessments (Profile of Mood States – POMS) were collected at four time points: pre-training (T-0), and after each training week (T-1, T-2, T-3). Analyzed biomarkers included cortisol, testosterone, catecholamines, hs-CRP, creatine kinase, and metabolic markers. Data were analyzed using repeated measures statistical tests to assess time-dependent changes. Cortisol and hs-CRP increased significantly after the first week (p < 0.01), while testosterone and catecholamines remained stable. Creatine kinase showed a persistent rise (p < 0.01), indicating muscle damage. Psychological assessments revealed increased fatigue, tension, and confusion, with reduced vigor (p < 0.05). Despite biochemical markers partially recovering, subjective fatigue persisted, highlighting a disconnect between physiological and psychological recovery. These findings highlight the importance of integrated monitoring strategies that include both biochemical and psychological assessments. The divergence between physiological and psychological recovery underscores the complexity of athlete fatigue and the need for integrated recovery interventions. Future research should explore long-term adaptations, sex-based differences, and targeted recovery strategies to optimize training, performance, and well-being in MMA athletes.
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spelling doaj-art-cdc1aee9a77a41f7a7fa716aa823e4ee2025-08-20T03:46:04ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-09719-zBiochemical and psychological markers of fatigue and recovery in mixed martial arts athletes during strength and conditioning trainingJoanna Ostapiuk-Karolczuk0Hanna Dziewiecka1Patryk Bojsa2Mirosława Cieślicka3Monika Zawadka-Kunikowska4Kaźmierczak Wojciech5Anna Kasperska6Department of Biological Sciences, Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow WielkopolskiDepartment of Biological Sciences, Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow WielkopolskiDepartment of Biological Sciences, Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow WielkopolskiDepartment of Human Physiology, Nicolaus Copernicus University, Ludwik Rydygier Collegium Medicum in BydgoszczDepartment of Human Physiology, Nicolaus Copernicus University, Ludwik Rydygier Collegium Medicum in BydgoszczDepartment of Human Physiology, Nicolaus Copernicus University, Ludwik Rydygier Collegium Medicum in BydgoszczDepartment of Biological Sciences, Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow WielkopolskiAbstract Mixed martial arts (MMA) training imposes significant physiological and psychological demands, increasing the risk of overtraining. This study aimed to assess temporal changes in biochemical and psychological fatigue and recovery markers during a structured 3-week strength and conditioning training program in professional MMA athletes. Ten male MMA athletes (26.2 ± 0.9 years; 7.0 ± 2.0 years of training experience) participated. Blood samples and psychological assessments (Profile of Mood States – POMS) were collected at four time points: pre-training (T-0), and after each training week (T-1, T-2, T-3). Analyzed biomarkers included cortisol, testosterone, catecholamines, hs-CRP, creatine kinase, and metabolic markers. Data were analyzed using repeated measures statistical tests to assess time-dependent changes. Cortisol and hs-CRP increased significantly after the first week (p < 0.01), while testosterone and catecholamines remained stable. Creatine kinase showed a persistent rise (p < 0.01), indicating muscle damage. Psychological assessments revealed increased fatigue, tension, and confusion, with reduced vigor (p < 0.05). Despite biochemical markers partially recovering, subjective fatigue persisted, highlighting a disconnect between physiological and psychological recovery. These findings highlight the importance of integrated monitoring strategies that include both biochemical and psychological assessments. The divergence between physiological and psychological recovery underscores the complexity of athlete fatigue and the need for integrated recovery interventions. Future research should explore long-term adaptations, sex-based differences, and targeted recovery strategies to optimize training, performance, and well-being in MMA athletes.https://doi.org/10.1038/s41598-025-09719-zMMA athletesHormonesInflammationMood statesFatigue
spellingShingle Joanna Ostapiuk-Karolczuk
Hanna Dziewiecka
Patryk Bojsa
Mirosława Cieślicka
Monika Zawadka-Kunikowska
Kaźmierczak Wojciech
Anna Kasperska
Biochemical and psychological markers of fatigue and recovery in mixed martial arts athletes during strength and conditioning training
Scientific Reports
MMA athletes
Hormones
Inflammation
Mood states
Fatigue
title Biochemical and psychological markers of fatigue and recovery in mixed martial arts athletes during strength and conditioning training
title_full Biochemical and psychological markers of fatigue and recovery in mixed martial arts athletes during strength and conditioning training
title_fullStr Biochemical and psychological markers of fatigue and recovery in mixed martial arts athletes during strength and conditioning training
title_full_unstemmed Biochemical and psychological markers of fatigue and recovery in mixed martial arts athletes during strength and conditioning training
title_short Biochemical and psychological markers of fatigue and recovery in mixed martial arts athletes during strength and conditioning training
title_sort biochemical and psychological markers of fatigue and recovery in mixed martial arts athletes during strength and conditioning training
topic MMA athletes
Hormones
Inflammation
Mood states
Fatigue
url https://doi.org/10.1038/s41598-025-09719-z
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