Tissue Volume and Section Area Changes of Paraffin and Methacrylate Embedded Testicular Sections: An Unbiased Design Study
For stereological estimates of structures in an organ such as total number (particles), length or surface area, the tissue change after processing (usually embedding of tissue blocks, sectioning of embedded blocks, and mounting and staining of sections) may need to be estimated to correct the resul...
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| Language: | English |
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Slovenian Society for Stereology and Quantitative Image Analysis
2024-11-01
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| Series: | Image Analysis and Stereology |
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| Online Access: | https://www.ias-iss.org/ojs/IAS/article/view/2951 |
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| author | Yang Guo Yu Xiang Dan Wang Zheng-Wei Yang |
| author_facet | Yang Guo Yu Xiang Dan Wang Zheng-Wei Yang |
| author_sort | Yang Guo |
| collection | DOAJ |
| description |
For stereological estimates of structures in an organ such as total number (particles), length or surface area, the tissue change after processing (usually embedding of tissue blocks, sectioning of embedded blocks, and mounting and staining of sections) may need to be estimated to correct the results obtained on final sections so as to reflect the true results in the organ before processing. We proposed that the correction be made depending on the stereological principle used: correction for estimation with 3-dimensional and 2-dimensional measurements be based on different tissue volume and section area changes. Previous researches on tissue changes were limited and overlooked different corrections. Using paraffin and methacrylate embedded testicular sections, we studied, with an unbiased design, the tissue volume and section area changes after processing. The results demonstrated that the overall change of the actual tissue volume of paraffin sections was a marked shrinkage of 21%-23% while that of methacrylate sections was a small expansion almost negligible; compared with area of the embedded block face, the final section area had a 4% decrease in paraffin but an 8% increase in methacrylate. The results were tentatively used for correction of estimates in some of previous studies. The present study provides both methods and data for experiments involving the study of tissue change for better stereological estimation.
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| format | Article |
| id | doaj-art-cda79041e4cc4dc79aab039a160fa90a |
| institution | Kabale University |
| issn | 1580-3139 1854-5165 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Slovenian Society for Stereology and Quantitative Image Analysis |
| record_format | Article |
| series | Image Analysis and Stereology |
| spelling | doaj-art-cda79041e4cc4dc79aab039a160fa90a2024-12-05T13:32:51ZengSlovenian Society for Stereology and Quantitative Image AnalysisImage Analysis and Stereology1580-31391854-51652024-11-0143310.5566/ias.2951Tissue Volume and Section Area Changes of Paraffin and Methacrylate Embedded Testicular Sections: An Unbiased Design StudyYang GuoYu XiangDan WangZheng-Wei Yang0https://orcid.org/0000-0003-1206-6982North Sichuan Medical College For stereological estimates of structures in an organ such as total number (particles), length or surface area, the tissue change after processing (usually embedding of tissue blocks, sectioning of embedded blocks, and mounting and staining of sections) may need to be estimated to correct the results obtained on final sections so as to reflect the true results in the organ before processing. We proposed that the correction be made depending on the stereological principle used: correction for estimation with 3-dimensional and 2-dimensional measurements be based on different tissue volume and section area changes. Previous researches on tissue changes were limited and overlooked different corrections. Using paraffin and methacrylate embedded testicular sections, we studied, with an unbiased design, the tissue volume and section area changes after processing. The results demonstrated that the overall change of the actual tissue volume of paraffin sections was a marked shrinkage of 21%-23% while that of methacrylate sections was a small expansion almost negligible; compared with area of the embedded block face, the final section area had a 4% decrease in paraffin but an 8% increase in methacrylate. The results were tentatively used for correction of estimates in some of previous studies. The present study provides both methods and data for experiments involving the study of tissue change for better stereological estimation. https://www.ias-iss.org/ojs/IAS/article/view/2951methacrylate sectionsparaffin sectionssection thicknesstissue shrinkagestereology |
| spellingShingle | Yang Guo Yu Xiang Dan Wang Zheng-Wei Yang Tissue Volume and Section Area Changes of Paraffin and Methacrylate Embedded Testicular Sections: An Unbiased Design Study Image Analysis and Stereology methacrylate sections paraffin sections section thickness tissue shrinkage stereology |
| title | Tissue Volume and Section Area Changes of Paraffin and Methacrylate Embedded Testicular Sections: An Unbiased Design Study |
| title_full | Tissue Volume and Section Area Changes of Paraffin and Methacrylate Embedded Testicular Sections: An Unbiased Design Study |
| title_fullStr | Tissue Volume and Section Area Changes of Paraffin and Methacrylate Embedded Testicular Sections: An Unbiased Design Study |
| title_full_unstemmed | Tissue Volume and Section Area Changes of Paraffin and Methacrylate Embedded Testicular Sections: An Unbiased Design Study |
| title_short | Tissue Volume and Section Area Changes of Paraffin and Methacrylate Embedded Testicular Sections: An Unbiased Design Study |
| title_sort | tissue volume and section area changes of paraffin and methacrylate embedded testicular sections an unbiased design study |
| topic | methacrylate sections paraffin sections section thickness tissue shrinkage stereology |
| url | https://www.ias-iss.org/ojs/IAS/article/view/2951 |
| work_keys_str_mv | AT yangguo tissuevolumeandsectionareachangesofparaffinandmethacrylateembeddedtesticularsectionsanunbiaseddesignstudy AT yuxiang tissuevolumeandsectionareachangesofparaffinandmethacrylateembeddedtesticularsectionsanunbiaseddesignstudy AT danwang tissuevolumeandsectionareachangesofparaffinandmethacrylateembeddedtesticularsectionsanunbiaseddesignstudy AT zhengweiyang tissuevolumeandsectionareachangesofparaffinandmethacrylateembeddedtesticularsectionsanunbiaseddesignstudy |