A potential antifungal bioproduct for Microsporum canis: Bee venom
Natural treatment options for Microsporum canis dermatophytosis are being explored because of resistance to several antifungal medications. In this study, the potential antifungal effect of bee venom (BV), a natural antimicrobial agent, on M. canis was investigated. The antifungal effects of BV, flu...
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| Format: | Article |
| Language: | English |
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AOSIS
2024-12-01
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| Series: | Onderstepoort Journal of Veterinary Research |
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| Online Access: | https://ojvr.org/index.php/ojvr/article/view/2191 |
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| author | Armağan E. Ütük Tülin Güven Gökmen Hatice Yazgan Funda Eşki Nevin Turut Şifa Karahan İbrahim Kıvrak Sedat Sevin Osman Sezer |
| author_facet | Armağan E. Ütük Tülin Güven Gökmen Hatice Yazgan Funda Eşki Nevin Turut Şifa Karahan İbrahim Kıvrak Sedat Sevin Osman Sezer |
| author_sort | Armağan E. Ütük |
| collection | DOAJ |
| description | Natural treatment options for Microsporum canis dermatophytosis are being explored because of resistance to several antifungal medications. In this study, the potential antifungal effect of bee venom (BV), a natural antimicrobial agent, on M. canis was investigated. The antifungal effects of BV, fluconazole, itraconazole, amphotericin B and terbinafine were evaluated by the macrodilution method at various concentrations by modifying the microdilution method recommended by the European Committee on Antimicrobial Susceptibility Testing. All isolates were observed to be susceptible to terbinafine and fully resistant to fluconazole and amphotericin B. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of M. canis isolate 2 (Mc2) were determined as 8 µg/mL for itraconazole. The MIC and MFC values of BV were found to be 320 µg/mL for the Mc2 isolate and 640 µg/mL for the Mc6 isolate. The results showed that the isolates obtained from clinical samples in this study were highly resistant to all antifungal agents, except terbinafine. The increase in resistance indicates that antifungal drugs will become insufficient and ineffective over time and natural products such as BV should be evaluated as alternatives.
Contribution: Although there are many drugs for the treatment of M. canis, the increase in resistance to antifungal agents reveals the need for the identification and development of new natural agents. Bee venom, which has been shown to have a safe and weak allergenic effect in various studies, can be tested for usability as a local antifungal drug when supported by in vivo studies. |
| format | Article |
| id | doaj-art-cd619b36cdd14f5a9d80b73b5779d3d4 |
| institution | Kabale University |
| issn | 0030-2465 2219-0635 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Onderstepoort Journal of Veterinary Research |
| spelling | doaj-art-cd619b36cdd14f5a9d80b73b5779d3d42024-12-18T12:43:01ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352024-12-01911e1e610.4102/ojvr.v91i1.2191722A potential antifungal bioproduct for Microsporum canis: Bee venomArmağan E. Ütük0Tülin Güven Gökmen1Hatice Yazgan2Funda Eşki3Nevin Turut4Şifa Karahan5İbrahim Kıvrak6Sedat Sevin7Osman Sezer8Department of Parasitology, Ceyhan Veterinary Faculty, Cukurova University, Adana, Türkiye; and Department of Biotechnology, Institute of Natural and Applied Sciences, Cukurova University, AdanaDepartment of Biotechnology, Institute of Natural and Applied Sciences, Cukurova University, Adana, Türkiye; and Department of Microbiology, Ceyhan Veterinary Faculty, Cukurova University, AdanaDepartment of Biotechnology, Institute of Natural and Applied Sciences, Cukurova University, Adana, Türkiye; and Department of Food Hygiene and Technology, Ceyhan Veterinary Faculty, Cukurova University, AdanaDepartment of Obstetrics and Gynecology, Ceyhan Veterinary Faculty, Cukurova University, AdanaBacteriology Laboratory, Adana Veterinary Control Institute, AdanaBacteriology Laboratory, Adana Veterinary Control Institute, AdanaDepartment of Chemistry and Chemical Processing Technologies, Cosmetic Technology Program, Muğla Vocational School, Muğla Sıtkı Koçman University, MuğlaDepartment of Pharmacology and Toxicology, Veterinary Faculty, Ankara University, AnkaraParasitology Laboratory, Adana Veterinary Control Institute, AdanaNatural treatment options for Microsporum canis dermatophytosis are being explored because of resistance to several antifungal medications. In this study, the potential antifungal effect of bee venom (BV), a natural antimicrobial agent, on M. canis was investigated. The antifungal effects of BV, fluconazole, itraconazole, amphotericin B and terbinafine were evaluated by the macrodilution method at various concentrations by modifying the microdilution method recommended by the European Committee on Antimicrobial Susceptibility Testing. All isolates were observed to be susceptible to terbinafine and fully resistant to fluconazole and amphotericin B. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of M. canis isolate 2 (Mc2) were determined as 8 µg/mL for itraconazole. The MIC and MFC values of BV were found to be 320 µg/mL for the Mc2 isolate and 640 µg/mL for the Mc6 isolate. The results showed that the isolates obtained from clinical samples in this study were highly resistant to all antifungal agents, except terbinafine. The increase in resistance indicates that antifungal drugs will become insufficient and ineffective over time and natural products such as BV should be evaluated as alternatives. Contribution: Although there are many drugs for the treatment of M. canis, the increase in resistance to antifungal agents reveals the need for the identification and development of new natural agents. Bee venom, which has been shown to have a safe and weak allergenic effect in various studies, can be tested for usability as a local antifungal drug when supported by in vivo studies.https://ojvr.org/index.php/ojvr/article/view/2191bee venommicrosporum canisantifungal activitybroth dilution methodbioproduct |
| spellingShingle | Armağan E. Ütük Tülin Güven Gökmen Hatice Yazgan Funda Eşki Nevin Turut Şifa Karahan İbrahim Kıvrak Sedat Sevin Osman Sezer A potential antifungal bioproduct for Microsporum canis: Bee venom Onderstepoort Journal of Veterinary Research bee venom microsporum canis antifungal activity broth dilution method bioproduct |
| title | A potential antifungal bioproduct for Microsporum canis: Bee venom |
| title_full | A potential antifungal bioproduct for Microsporum canis: Bee venom |
| title_fullStr | A potential antifungal bioproduct for Microsporum canis: Bee venom |
| title_full_unstemmed | A potential antifungal bioproduct for Microsporum canis: Bee venom |
| title_short | A potential antifungal bioproduct for Microsporum canis: Bee venom |
| title_sort | potential antifungal bioproduct for microsporum canis bee venom |
| topic | bee venom microsporum canis antifungal activity broth dilution method bioproduct |
| url | https://ojvr.org/index.php/ojvr/article/view/2191 |
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