Identifying Hub Genes and miRNAs Associated with Alzheimer’s Disease: A Bioinformatics Pathway to Novel Therapeutic Strategies
Alzheimer’s disease (AD) is a neurodegenerative disorder that mainly affects the elderly population. It is characterized by cognitive impairment and dementia due to abnormal levels of amyloid beta peptide (Aβ) and axonal Tau protein in the brain. However, the complex underlying mechanisms affecting...
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| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/12/1641 |
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| author | Elisa Gascón Ana Cristina Calvo Nora Molina Pilar Zaragoza Rosario Osta |
| author_facet | Elisa Gascón Ana Cristina Calvo Nora Molina Pilar Zaragoza Rosario Osta |
| author_sort | Elisa Gascón |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) is a neurodegenerative disorder that mainly affects the elderly population. It is characterized by cognitive impairment and dementia due to abnormal levels of amyloid beta peptide (Aβ) and axonal Tau protein in the brain. However, the complex underlying mechanisms affecting this disease are not yet known, and there is a lack of standardized biomarkers and therapeutic targets. Therefore, in this study, by means of bioinformatics analysis, AD-affected brain tissue was analyzed using the GSE138260 dataset, identifying 612 differentially expressed genes (DEGs). Functional analysis revealed 388 upregulated DEGs associated with sensory perception and 224 downregulated DEGs linked to the regulation and modulation of synaptic processes. Protein–protein interaction network analysis identified 20 hub genes. Furthermore, miRNA target gene networks revealed 1767 miRNAs linked to hub genes, among which hsa-mir-106a-5p, hsa-mir-17-5p, hsa-mir-26a-5p, hsa-mir-27a-3p and hsa-mir-34a-5p were the most relevant. This study presents novel biomarkers and therapeutic targets for AD by analyzing the information obtained with a comprehensive literature review, providing new potential targets to study their role in AD. |
| format | Article |
| id | doaj-art-cd3eba41a7aa4f9b9dd1a804b955ff25 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-cd3eba41a7aa4f9b9dd1a804b955ff252024-12-27T14:14:02ZengMDPI AGBiomolecules2218-273X2024-12-011412164110.3390/biom14121641Identifying Hub Genes and miRNAs Associated with Alzheimer’s Disease: A Bioinformatics Pathway to Novel Therapeutic StrategiesElisa Gascón0Ana Cristina Calvo1Nora Molina2Pilar Zaragoza3Rosario Osta4Department of Anatomy, Embryology and Animal Genetics, University of Zaragoza, 50013 Zaragoza, SpainDepartment of Anatomy, Embryology and Animal Genetics, University of Zaragoza, 50013 Zaragoza, SpainDepartment of Anatomy, Embryology and Animal Genetics, University of Zaragoza, 50013 Zaragoza, SpainDepartment of Anatomy, Embryology and Animal Genetics, University of Zaragoza, 50013 Zaragoza, SpainDepartment of Anatomy, Embryology and Animal Genetics, University of Zaragoza, 50013 Zaragoza, SpainAlzheimer’s disease (AD) is a neurodegenerative disorder that mainly affects the elderly population. It is characterized by cognitive impairment and dementia due to abnormal levels of amyloid beta peptide (Aβ) and axonal Tau protein in the brain. However, the complex underlying mechanisms affecting this disease are not yet known, and there is a lack of standardized biomarkers and therapeutic targets. Therefore, in this study, by means of bioinformatics analysis, AD-affected brain tissue was analyzed using the GSE138260 dataset, identifying 612 differentially expressed genes (DEGs). Functional analysis revealed 388 upregulated DEGs associated with sensory perception and 224 downregulated DEGs linked to the regulation and modulation of synaptic processes. Protein–protein interaction network analysis identified 20 hub genes. Furthermore, miRNA target gene networks revealed 1767 miRNAs linked to hub genes, among which hsa-mir-106a-5p, hsa-mir-17-5p, hsa-mir-26a-5p, hsa-mir-27a-3p and hsa-mir-34a-5p were the most relevant. This study presents novel biomarkers and therapeutic targets for AD by analyzing the information obtained with a comprehensive literature review, providing new potential targets to study their role in AD.https://www.mdpi.com/2218-273X/14/12/1641bioinformaticsomicsneurodegenerative diseases (NDDs)extracellular/circulating biomarkersmicroRNAAlzheimer |
| spellingShingle | Elisa Gascón Ana Cristina Calvo Nora Molina Pilar Zaragoza Rosario Osta Identifying Hub Genes and miRNAs Associated with Alzheimer’s Disease: A Bioinformatics Pathway to Novel Therapeutic Strategies Biomolecules bioinformatics omics neurodegenerative diseases (NDDs) extracellular/circulating biomarkers microRNA Alzheimer |
| title | Identifying Hub Genes and miRNAs Associated with Alzheimer’s Disease: A Bioinformatics Pathway to Novel Therapeutic Strategies |
| title_full | Identifying Hub Genes and miRNAs Associated with Alzheimer’s Disease: A Bioinformatics Pathway to Novel Therapeutic Strategies |
| title_fullStr | Identifying Hub Genes and miRNAs Associated with Alzheimer’s Disease: A Bioinformatics Pathway to Novel Therapeutic Strategies |
| title_full_unstemmed | Identifying Hub Genes and miRNAs Associated with Alzheimer’s Disease: A Bioinformatics Pathway to Novel Therapeutic Strategies |
| title_short | Identifying Hub Genes and miRNAs Associated with Alzheimer’s Disease: A Bioinformatics Pathway to Novel Therapeutic Strategies |
| title_sort | identifying hub genes and mirnas associated with alzheimer s disease a bioinformatics pathway to novel therapeutic strategies |
| topic | bioinformatics omics neurodegenerative diseases (NDDs) extracellular/circulating biomarkers microRNA Alzheimer |
| url | https://www.mdpi.com/2218-273X/14/12/1641 |
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