Study on the Mechanism of Flos Carthami Treatment for Chronic Alcoholic Liver Injury Using BP Neural Network Algorithm Combined with UHPLC-MS Technology
Flos Carthami (FC) has a good therapeutic effect on chronic alcoholic liver injury (CALI) in clinical practice, but the treatment mechanism is not very clear. Therefore, elucidating the molecular mechanism of action of FC in treating CALI is of great significance for the further development and appl...
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Editorial Board of Journal of Chinese Mass Spectrometry Society
2024-11-01
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| Series: | Zhipu Xuebao |
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| Online Access: | https://zpxb.xml-journal.net/article/doi/10.7538/zpxb.2024.1004 |
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| author | Xi-ye WANG Xiao-jing HAN Ming-yang JIANG Mei-rong BAI Liang XU |
| author_facet | Xi-ye WANG Xiao-jing HAN Ming-yang JIANG Mei-rong BAI Liang XU |
| author_sort | Xi-ye WANG |
| collection | DOAJ |
| description | Flos Carthami (FC) has a good therapeutic effect on chronic alcoholic liver injury (CALI) in clinical practice, but the treatment mechanism is not very clear. Therefore, elucidating the molecular mechanism of action of FC in treating CALI is of great significance for the further development and application of drugs. Male Wistar rats were as the research object. The model group rats were orally administered with 8 mL/kg alcohol for 28 days to establish a CALI model, while the treatment group rats were orally administered with high (4.2903 g/kg), medium (1.4301 g/kg), and low (0.4767 g/kg) doses of FC extract. Potential biomarkers related to CALI were identified using rat serum metabonomics analysis methods combined with ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS) technology, and the regulatory mechanisms of FC on these biomarkers were investigated. The BP neural network model was established by MATLAB software to deal with the classification problem of omics data. The H&E staining experiment found that after gavage, high-dose FC extract can reduce the degree of liver cell damage. Compared with the model group, the expression levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the high-dose FC group decrease, indicating that the high-dose FC extract has liver protective effect. The classification accuracy of the BP neural network model is 95.8%, and the classification effect is good. Through volcanic map analysis, a total of twenty biomarkers related to CALI are identified, and FC can have a callback effect on these biomarkers. The results indicated that FC may exert therapeutic effect on CALI by regulating the metabolism of triglycerides, fatty acids, phospholipids, bile acids, amino acids and Vitamin E. This study provides a theoretical foundation for the promotion and clinical application. |
| format | Article |
| id | doaj-art-cd0b43e54c274de595eef0de5fa499a8 |
| institution | Kabale University |
| issn | 1004-2997 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Editorial Board of Journal of Chinese Mass Spectrometry Society |
| record_format | Article |
| series | Zhipu Xuebao |
| spelling | doaj-art-cd0b43e54c274de595eef0de5fa499a82024-11-20T03:03:57ZengEditorial Board of Journal of Chinese Mass Spectrometry SocietyZhipu Xuebao1004-29972024-11-0145689790610.7538/zpxb.2024.10042024-004-jn12Study on the Mechanism of Flos Carthami Treatment for Chronic Alcoholic Liver Injury Using BP Neural Network Algorithm Combined with UHPLC-MS TechnologyXi-ye WANG0Xiao-jing HAN1Ming-yang JIANG2Mei-rong BAI3Liang XU4Inner Mongolia Key Laboratory for the Natural Products Chemistry and Functional Molecular Synthesis, College of Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao 028000, ChinaKey Laboratory of Mongolian Research and Development Project in Ministry of Education, Mongolian Medical College, Inner Mongolia Minzu University, Tongliao 028000, ChinaCollege of Computer Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, ChinaKey Laboratory of Mongolian Research and Development Project in Ministry of Education, Mongolian Medical College, Inner Mongolia Minzu University, Tongliao 028000, ChinaInner Mongolia Key Laboratory for the Natural Products Chemistry and Functional Molecular Synthesis, College of Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao 028000, ChinaFlos Carthami (FC) has a good therapeutic effect on chronic alcoholic liver injury (CALI) in clinical practice, but the treatment mechanism is not very clear. Therefore, elucidating the molecular mechanism of action of FC in treating CALI is of great significance for the further development and application of drugs. Male Wistar rats were as the research object. The model group rats were orally administered with 8 mL/kg alcohol for 28 days to establish a CALI model, while the treatment group rats were orally administered with high (4.2903 g/kg), medium (1.4301 g/kg), and low (0.4767 g/kg) doses of FC extract. Potential biomarkers related to CALI were identified using rat serum metabonomics analysis methods combined with ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS) technology, and the regulatory mechanisms of FC on these biomarkers were investigated. The BP neural network model was established by MATLAB software to deal with the classification problem of omics data. The H&E staining experiment found that after gavage, high-dose FC extract can reduce the degree of liver cell damage. Compared with the model group, the expression levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the high-dose FC group decrease, indicating that the high-dose FC extract has liver protective effect. The classification accuracy of the BP neural network model is 95.8%, and the classification effect is good. Through volcanic map analysis, a total of twenty biomarkers related to CALI are identified, and FC can have a callback effect on these biomarkers. The results indicated that FC may exert therapeutic effect on CALI by regulating the metabolism of triglycerides, fatty acids, phospholipids, bile acids, amino acids and Vitamin E. This study provides a theoretical foundation for the promotion and clinical application.https://zpxb.xml-journal.net/article/doi/10.7538/zpxb.2024.1004flos carthamichronic alcoholic liver injury (cali)metabonomicsultra-high performance liquid chromatography mass spectrometry (uhplc-ms)bp neural network algorithm |
| spellingShingle | Xi-ye WANG Xiao-jing HAN Ming-yang JIANG Mei-rong BAI Liang XU Study on the Mechanism of Flos Carthami Treatment for Chronic Alcoholic Liver Injury Using BP Neural Network Algorithm Combined with UHPLC-MS Technology Zhipu Xuebao flos carthami chronic alcoholic liver injury (cali) metabonomics ultra-high performance liquid chromatography mass spectrometry (uhplc-ms) bp neural network algorithm |
| title | Study on the Mechanism of Flos Carthami Treatment for Chronic Alcoholic Liver Injury Using BP Neural Network Algorithm Combined with UHPLC-MS Technology |
| title_full | Study on the Mechanism of Flos Carthami Treatment for Chronic Alcoholic Liver Injury Using BP Neural Network Algorithm Combined with UHPLC-MS Technology |
| title_fullStr | Study on the Mechanism of Flos Carthami Treatment for Chronic Alcoholic Liver Injury Using BP Neural Network Algorithm Combined with UHPLC-MS Technology |
| title_full_unstemmed | Study on the Mechanism of Flos Carthami Treatment for Chronic Alcoholic Liver Injury Using BP Neural Network Algorithm Combined with UHPLC-MS Technology |
| title_short | Study on the Mechanism of Flos Carthami Treatment for Chronic Alcoholic Liver Injury Using BP Neural Network Algorithm Combined with UHPLC-MS Technology |
| title_sort | study on the mechanism of flos carthami treatment for chronic alcoholic liver injury using bp neural network algorithm combined with uhplc ms technology |
| topic | flos carthami chronic alcoholic liver injury (cali) metabonomics ultra-high performance liquid chromatography mass spectrometry (uhplc-ms) bp neural network algorithm |
| url | https://zpxb.xml-journal.net/article/doi/10.7538/zpxb.2024.1004 |
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