Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired Pneumonia
Objective: This study aims to determine the frequency of pathogen detection by real-time polymerase chain reaction (PCR), the frequency of pathogen isolation by culture; and compare the value of real-time PCR and culture of nasopharyngeal aspiration samples in patients with severe community-acquired...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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AVES Yayincilik
2024-07-01
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| Series: | Turkish Archives of Pediatrics |
| Online Access: | https://turkarchpediatr.org/en/comparison-of-real-time-polymerase-chain-reaction-and-culture-for-targeting-pathogens-in-pediatric-severe-community-acquired-pneumonia-131668 |
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| _version_ | 1846151425621491712 |
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| author | Khai Quang Tran Van Hung Pham Chau Minh Vo Quan Minh Pham Phuong Minh Nguyen |
| author_facet | Khai Quang Tran Van Hung Pham Chau Minh Vo Quan Minh Pham Phuong Minh Nguyen |
| author_sort | Khai Quang Tran |
| collection | DOAJ |
| description | Objective: This study aims to determine the frequency of pathogen detection by real-time polymerase chain reaction (PCR), the frequency of pathogen isolation by culture; and compare the value of real-time PCR and culture of nasopharyngeal aspiration samples in patients with severe community-acquired pneumonia (sCAP).
Materials and Methods: It was a prospective and descriptive study. All pediatric patients diagnosed with sCAP were performed real-time PCR and culture of nasopharyngeal aspiration samples.
Results: A total of 336 patient samples were obtained from children with sCAP. Real-time PCR detected pathogens in 312 patients (92.9%), while culture isolated bacteria in 228 patients (67.9%). Coinfections were reported in 279 cases (83.0%) through real-time PCR. The frequency of agreement between culture and real-time PCR was quite high (P < .001).
Conclusion: Real-time PCR demonstrated more ability for detecting microorganisms than culture. This finding highlighted the value of real-time PCR for targeting pathogens in children with sCAP, particularly in cases involving complex pathogens or those requiring timely identification. |
| format | Article |
| id | doaj-art-cd040bc589664d358e176e5cfaeec0a5 |
| institution | Kabale University |
| issn | 2757-6256 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | AVES Yayincilik |
| record_format | Article |
| series | Turkish Archives of Pediatrics |
| spelling | doaj-art-cd040bc589664d358e176e5cfaeec0a52024-11-27T12:56:57ZengAVES YayincilikTurkish Archives of Pediatrics2757-62562024-07-0159438338910.5152/TurkArchPediatr.2024.24005Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired PneumoniaKhai Quang Tran0Van Hung Pham1Chau Minh Vo2Quan Minh Pham3Phuong Minh Nguyen4Department of Pediatrics, Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho City, VietnamLaboratory of Nam Khoa Biotek Company, Vietnam Research and Development Institute of Clinical Microbiology, Ho Chi Minh City, VietnamDepartment of Pediatrics, Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho City, VietnamDepartment of Pediatrics, Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho City, VietnamDepartment of Pediatrics, Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho City, VietnamObjective: This study aims to determine the frequency of pathogen detection by real-time polymerase chain reaction (PCR), the frequency of pathogen isolation by culture; and compare the value of real-time PCR and culture of nasopharyngeal aspiration samples in patients with severe community-acquired pneumonia (sCAP). Materials and Methods: It was a prospective and descriptive study. All pediatric patients diagnosed with sCAP were performed real-time PCR and culture of nasopharyngeal aspiration samples. Results: A total of 336 patient samples were obtained from children with sCAP. Real-time PCR detected pathogens in 312 patients (92.9%), while culture isolated bacteria in 228 patients (67.9%). Coinfections were reported in 279 cases (83.0%) through real-time PCR. The frequency of agreement between culture and real-time PCR was quite high (P < .001). Conclusion: Real-time PCR demonstrated more ability for detecting microorganisms than culture. This finding highlighted the value of real-time PCR for targeting pathogens in children with sCAP, particularly in cases involving complex pathogens or those requiring timely identification.https://turkarchpediatr.org/en/comparison-of-real-time-polymerase-chain-reaction-and-culture-for-targeting-pathogens-in-pediatric-severe-community-acquired-pneumonia-131668 |
| spellingShingle | Khai Quang Tran Van Hung Pham Chau Minh Vo Quan Minh Pham Phuong Minh Nguyen Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired Pneumonia Turkish Archives of Pediatrics |
| title | Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired Pneumonia |
| title_full | Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired Pneumonia |
| title_fullStr | Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired Pneumonia |
| title_full_unstemmed | Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired Pneumonia |
| title_short | Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired Pneumonia |
| title_sort | comparison of real time polymerase chain reaction and culture for targeting pathogens in pediatric severe community acquired pneumonia |
| url | https://turkarchpediatr.org/en/comparison-of-real-time-polymerase-chain-reaction-and-culture-for-targeting-pathogens-in-pediatric-severe-community-acquired-pneumonia-131668 |
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