A sequencing-based method for quantifying gene-deletion mutants of bacteria in the intracellular environment

A sequencing-based method for quantifying gene-deletion mutants of bacteria in the intracellular environment

Advancements in next-generation sequencing (NGS) have significantly accelerated the development of innovative methodologies in microbiological research. In this study, we present a novel method to quantify the net survival of gene-deletion mutants within the intracellular environment. Based on stand...

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Bibliographic Details
Main Authors: Xiao Fei, Zengzhi Yuan, Sandra Marina Wellner, Yibing Ma, John Elmerdahl Olsen
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Microbiology
Subjects:
next-generation sequencing
intracellular survival
gene-deletion mutants
Salmonella
bacteria
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1487724/full
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