A VioA Variant Activates Antibiotic Streptogramins in the Heterologous Host <i>Streptomyces</i> sp. OUC20-O
Heterologous expression of the G231L variant of VioA into 16 strains of marine-derived <i>Streptomyces</i>, combined with bioactivity tracking, leads to the activation of seven antibiotic streptogramins (<b>1</b>–<b>7</b>) in <i>Streptomyces</i> sp. OU...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-05-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/23/5/205 |
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| author | Jie Shan Liangguang Yue Luyao Xu Runyi Wang Qingzhou Meng Jun Feng Joon-Hee Lee Ming Lu Huayue Li |
| author_facet | Jie Shan Liangguang Yue Luyao Xu Runyi Wang Qingzhou Meng Jun Feng Joon-Hee Lee Ming Lu Huayue Li |
| author_sort | Jie Shan |
| collection | DOAJ |
| description | Heterologous expression of the G231L variant of VioA into 16 strains of marine-derived <i>Streptomyces</i>, combined with bioactivity tracking, leads to the activation of seven antibiotic streptogramins (<b>1</b>–<b>7</b>) in <i>Streptomyces</i> sp. OUC20-O. Among these, compound <b>1</b>, named linstreptogramin, is a new compound with an unusual linear streptogramin skeleton. The planar structure and stereochemistry of compound <b>1</b> were established based on extensive MS and NMR spectroscopic analyses, together with ECD calculations. In the antibacterial activity evaluation, compounds <b>1</b>–<b>4</b> showed significant growth inhibition against the multidrug-resistant <i>Enterococcus faecium</i> CCARM 5203 with MIC values of 0.2–1.6 µg/mL, which are comparable to the positive control vancomycin. |
| format | Article |
| id | doaj-art-cca35d2f417e4dacbde2ee37d14bf895 |
| institution | Kabale University |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-cca35d2f417e4dacbde2ee37d14bf8952025-08-20T03:47:57ZengMDPI AGMarine Drugs1660-33972025-05-0123520510.3390/md23050205A VioA Variant Activates Antibiotic Streptogramins in the Heterologous Host <i>Streptomyces</i> sp. OUC20-OJie Shan0Liangguang Yue1Luyao Xu2Runyi Wang3Qingzhou Meng4Jun Feng5Joon-Hee Lee6Ming Lu7Huayue Li8Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaState Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Qingdao New Energy Shandong Laboratory, Shandong C1 Refinery Engineering Research Center, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, ChinaCollege of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaState Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Qingdao New Energy Shandong Laboratory, Shandong C1 Refinery Engineering Research Center, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, ChinaKey Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaHeterologous expression of the G231L variant of VioA into 16 strains of marine-derived <i>Streptomyces</i>, combined with bioactivity tracking, leads to the activation of seven antibiotic streptogramins (<b>1</b>–<b>7</b>) in <i>Streptomyces</i> sp. OUC20-O. Among these, compound <b>1</b>, named linstreptogramin, is a new compound with an unusual linear streptogramin skeleton. The planar structure and stereochemistry of compound <b>1</b> were established based on extensive MS and NMR spectroscopic analyses, together with ECD calculations. In the antibacterial activity evaluation, compounds <b>1</b>–<b>4</b> showed significant growth inhibition against the multidrug-resistant <i>Enterococcus faecium</i> CCARM 5203 with MIC values of 0.2–1.6 µg/mL, which are comparable to the positive control vancomycin.https://www.mdpi.com/1660-3397/23/5/205heterologous expressionmarine-derived <i>Streptomyces</i>multidrug-resistantantibioticstreptogramin |
| spellingShingle | Jie Shan Liangguang Yue Luyao Xu Runyi Wang Qingzhou Meng Jun Feng Joon-Hee Lee Ming Lu Huayue Li A VioA Variant Activates Antibiotic Streptogramins in the Heterologous Host <i>Streptomyces</i> sp. OUC20-O Marine Drugs heterologous expression marine-derived <i>Streptomyces</i> multidrug-resistant antibiotic streptogramin |
| title | A VioA Variant Activates Antibiotic Streptogramins in the Heterologous Host <i>Streptomyces</i> sp. OUC20-O |
| title_full | A VioA Variant Activates Antibiotic Streptogramins in the Heterologous Host <i>Streptomyces</i> sp. OUC20-O |
| title_fullStr | A VioA Variant Activates Antibiotic Streptogramins in the Heterologous Host <i>Streptomyces</i> sp. OUC20-O |
| title_full_unstemmed | A VioA Variant Activates Antibiotic Streptogramins in the Heterologous Host <i>Streptomyces</i> sp. OUC20-O |
| title_short | A VioA Variant Activates Antibiotic Streptogramins in the Heterologous Host <i>Streptomyces</i> sp. OUC20-O |
| title_sort | vioa variant activates antibiotic streptogramins in the heterologous host i streptomyces i sp ouc20 o |
| topic | heterologous expression marine-derived <i>Streptomyces</i> multidrug-resistant antibiotic streptogramin |
| url | https://www.mdpi.com/1660-3397/23/5/205 |
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