Limosilactobacillus reuteri fermented brown rice alleviates anxiety improves cognition and modulates gut microbiota in stressed mice
Abstract Chronic stress disrupts gut microbiota homeostasis, contributing to anxiety and depression. This study explored the effects of Limosilactobacillus reuteri fermented brown rice (FBR) on anxiety using an ICR mouse chronic mild stress (CMS) model. Anxiety was assessed through body weight, cort...
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Nature Portfolio
2025-01-01
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| Series: | npj Science of Food |
| Online Access: | https://doi.org/10.1038/s41538-025-00369-z |
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| author | Akanksha Tyagi Yu-Yeong Choi Lingyue Shan Annadurai Vinothkanna Eun-Seok Lee Ramachandran Chelliah Kaliyan Barathikannan Sivakumar Thasma Raman Se Jin Park Ai-Qun Jia Geun Pyo Choi Deog Hwan Oh |
| author_facet | Akanksha Tyagi Yu-Yeong Choi Lingyue Shan Annadurai Vinothkanna Eun-Seok Lee Ramachandran Chelliah Kaliyan Barathikannan Sivakumar Thasma Raman Se Jin Park Ai-Qun Jia Geun Pyo Choi Deog Hwan Oh |
| author_sort | Akanksha Tyagi |
| collection | DOAJ |
| description | Abstract Chronic stress disrupts gut microbiota homeostasis, contributing to anxiety and depression. This study explored the effects of Limosilactobacillus reuteri fermented brown rice (FBR) on anxiety using an ICR mouse chronic mild stress (CMS) model. Anxiety was assessed through body weight, corticosterone levels, neurotransmitter profiles, and behavioral tests. A four-week FBR regimen reduced corticosterone, restored neurotransmitters like gamma-aminobutyric acid (GABA) and serotonin, and improved anxiety-related behaviors. Metagenomic (16S rRNA) and metabolomic analyses revealed enhanced amino acid metabolism, energy metabolism, and short-chain fatty acid (SCFA) production in FBR-treated mice. FBR-enriched beneficial gut bacteria, aligning the microbiota profile with that of non-stressed mice. FBR also modulated GABA receptor-related gene expression, promoting relaxation. Network pharmacology identified quercetin, GABA, glutamic acid, phenylalanine, and ferulic acid as bioactive compounds with neuroprotective potential. These findings highlight FBR’s potential as a gut-brain axis-targeted therapeutic for anxiety and stress-related disorders. |
| format | Article |
| id | doaj-art-cc79b86c0b2147e29bef2e1dbeed9647 |
| institution | Kabale University |
| issn | 2396-8370 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Science of Food |
| spelling | doaj-art-cc79b86c0b2147e29bef2e1dbeed96472025-01-12T12:41:13ZengNature Portfolionpj Science of Food2396-83702025-01-019111710.1038/s41538-025-00369-zLimosilactobacillus reuteri fermented brown rice alleviates anxiety improves cognition and modulates gut microbiota in stressed miceAkanksha Tyagi0Yu-Yeong Choi1Lingyue Shan2Annadurai Vinothkanna3Eun-Seok Lee4Ramachandran Chelliah5Kaliyan Barathikannan6Sivakumar Thasma Raman7Se Jin Park8Ai-Qun Jia9Geun Pyo Choi10Deog Hwan Oh11Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue UniversityDepartment of Natural Resources and Environmental Science, Kangwon National UniversityDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National UniversitySchool of Chemistry and Chemical Engineering and Key Laboratory of Ministry of Education for Advanced Materials in Tropical Island Resources, Hainan UniversityDepartment of Natural Resources and Environmental Science, Kangwon National UniversityDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National UniversitySaveetha School of Engineering, Saveetha UniversitySchool of Food and Biological Engineering, Jiangsu UniversityDepartment of Natural Resources and Environmental Science, Kangwon National UniversityHainan General Hospital, Hainan Affiliated Hospital of Hainan Medical UniversityDepartment of Barista and Bakery, Gangwon State UniversityDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National UniversityAbstract Chronic stress disrupts gut microbiota homeostasis, contributing to anxiety and depression. This study explored the effects of Limosilactobacillus reuteri fermented brown rice (FBR) on anxiety using an ICR mouse chronic mild stress (CMS) model. Anxiety was assessed through body weight, corticosterone levels, neurotransmitter profiles, and behavioral tests. A four-week FBR regimen reduced corticosterone, restored neurotransmitters like gamma-aminobutyric acid (GABA) and serotonin, and improved anxiety-related behaviors. Metagenomic (16S rRNA) and metabolomic analyses revealed enhanced amino acid metabolism, energy metabolism, and short-chain fatty acid (SCFA) production in FBR-treated mice. FBR-enriched beneficial gut bacteria, aligning the microbiota profile with that of non-stressed mice. FBR also modulated GABA receptor-related gene expression, promoting relaxation. Network pharmacology identified quercetin, GABA, glutamic acid, phenylalanine, and ferulic acid as bioactive compounds with neuroprotective potential. These findings highlight FBR’s potential as a gut-brain axis-targeted therapeutic for anxiety and stress-related disorders.https://doi.org/10.1038/s41538-025-00369-z |
| spellingShingle | Akanksha Tyagi Yu-Yeong Choi Lingyue Shan Annadurai Vinothkanna Eun-Seok Lee Ramachandran Chelliah Kaliyan Barathikannan Sivakumar Thasma Raman Se Jin Park Ai-Qun Jia Geun Pyo Choi Deog Hwan Oh Limosilactobacillus reuteri fermented brown rice alleviates anxiety improves cognition and modulates gut microbiota in stressed mice npj Science of Food |
| title | Limosilactobacillus reuteri fermented brown rice alleviates anxiety improves cognition and modulates gut microbiota in stressed mice |
| title_full | Limosilactobacillus reuteri fermented brown rice alleviates anxiety improves cognition and modulates gut microbiota in stressed mice |
| title_fullStr | Limosilactobacillus reuteri fermented brown rice alleviates anxiety improves cognition and modulates gut microbiota in stressed mice |
| title_full_unstemmed | Limosilactobacillus reuteri fermented brown rice alleviates anxiety improves cognition and modulates gut microbiota in stressed mice |
| title_short | Limosilactobacillus reuteri fermented brown rice alleviates anxiety improves cognition and modulates gut microbiota in stressed mice |
| title_sort | limosilactobacillus reuteri fermented brown rice alleviates anxiety improves cognition and modulates gut microbiota in stressed mice |
| url | https://doi.org/10.1038/s41538-025-00369-z |
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