Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress
Abstract Background DnaJA proteins, a prominent subfamily of the DnaJ family, function as molecular chaperones that respond to various external stresses. Extensive studies on the DnaJ family have been conducted in plants. However, research on this subfamily in tobacco remains relatively scarce. Resu...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2025-01-01
|
| Series: | Chemical and Biological Technologies in Agriculture |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40538-024-00726-2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841544937858924544 |
|---|---|
| author | Qiao Wang Lijun Meng Zechao Qu Huan Su Jiemeng Tao Peng Lu Chenfeng Hua Jinbang Wang Jianfeng Zhang Peijian Cao Jingjing Jin |
| author_facet | Qiao Wang Lijun Meng Zechao Qu Huan Su Jiemeng Tao Peng Lu Chenfeng Hua Jinbang Wang Jianfeng Zhang Peijian Cao Jingjing Jin |
| author_sort | Qiao Wang |
| collection | DOAJ |
| description | Abstract Background DnaJA proteins, a prominent subfamily of the DnaJ family, function as molecular chaperones that respond to various external stresses. Extensive studies on the DnaJ family have been conducted in plants. However, research on this subfamily in tobacco remains relatively scarce. Results In this study, we identified 24 DnaJA genes in tobacco, and classified them into three individual groups. A comprehensive analysis based on gene structure, motif composition, and evolutionary pattern revealed the divergence of tobacco DnaJA genes. For the evolution of the NtDnaJA genes, purification selection was the major factor. In addition, the potential regulatory network unveiled that NtDnaJAs could be regulated by miRNAs and various transcription factors associated with diverse stress responses. Through expression pattern analysis of public RNA-seq datasets and qRT-PCR experiments, it was observed that many NtDnaJAs displayed tissue-specific expression and might play significant roles in different biotic and abiotic stresses. Additionally, the pivotal role of NtDnaJA3 in boosting plant drought resistance was confirmed. Conclusions This study provides important perspectives on the evolution of NtDnaJA genes and their involvement in stress responses, laying the groundwork for future research into the roles of DnaJA regulatory genes in tobacco. Graphical Abstract |
| format | Article |
| id | doaj-art-cc2bb3790342449ebccc02302ad5be3d |
| institution | Kabale University |
| issn | 2196-5641 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj-art-cc2bb3790342449ebccc02302ad5be3d2025-01-12T12:11:06ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412025-01-0112111510.1186/s40538-024-00726-2Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stressQiao Wang0Lijun Meng1Zechao Qu2Huan Su3Jiemeng Tao4Peng Lu5Chenfeng Hua6Jinbang Wang7Jianfeng Zhang8Peijian Cao9Jingjing Jin10Beijing Life Science AcademyChina Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTCChina Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTCBeijing Life Science AcademyBeijing Life Science AcademyBeijing Life Science AcademyBeijing Life Science AcademyChina Tobacco Science and Technology Information Center, Zhengzhou Tobacco Research Institute of CNTCBeijing Life Science AcademyBeijing Life Science AcademyBeijing Life Science AcademyAbstract Background DnaJA proteins, a prominent subfamily of the DnaJ family, function as molecular chaperones that respond to various external stresses. Extensive studies on the DnaJ family have been conducted in plants. However, research on this subfamily in tobacco remains relatively scarce. Results In this study, we identified 24 DnaJA genes in tobacco, and classified them into three individual groups. A comprehensive analysis based on gene structure, motif composition, and evolutionary pattern revealed the divergence of tobacco DnaJA genes. For the evolution of the NtDnaJA genes, purification selection was the major factor. In addition, the potential regulatory network unveiled that NtDnaJAs could be regulated by miRNAs and various transcription factors associated with diverse stress responses. Through expression pattern analysis of public RNA-seq datasets and qRT-PCR experiments, it was observed that many NtDnaJAs displayed tissue-specific expression and might play significant roles in different biotic and abiotic stresses. Additionally, the pivotal role of NtDnaJA3 in boosting plant drought resistance was confirmed. Conclusions This study provides important perspectives on the evolution of NtDnaJA genes and their involvement in stress responses, laying the groundwork for future research into the roles of DnaJA regulatory genes in tobacco. Graphical Abstracthttps://doi.org/10.1186/s40538-024-00726-2TobaccoDnaJAPhylogenetic analysisRegulatory networkExpression patternDrought stress |
| spellingShingle | Qiao Wang Lijun Meng Zechao Qu Huan Su Jiemeng Tao Peng Lu Chenfeng Hua Jinbang Wang Jianfeng Zhang Peijian Cao Jingjing Jin Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress Chemical and Biological Technologies in Agriculture Tobacco DnaJA Phylogenetic analysis Regulatory network Expression pattern Drought stress |
| title | Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress |
| title_full | Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress |
| title_fullStr | Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress |
| title_full_unstemmed | Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress |
| title_short | Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress |
| title_sort | genome wide analysis of dnaja proteins in nicotiana tabacum reveals ntdnaja3 responses to drought stress |
| topic | Tobacco DnaJA Phylogenetic analysis Regulatory network Expression pattern Drought stress |
| url | https://doi.org/10.1186/s40538-024-00726-2 |
| work_keys_str_mv | AT qiaowang genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress AT lijunmeng genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress AT zechaoqu genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress AT huansu genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress AT jiemengtao genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress AT penglu genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress AT chenfenghua genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress AT jinbangwang genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress AT jianfengzhang genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress AT peijiancao genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress AT jingjingjin genomewideanalysisofdnajaproteinsinnicotianatabacumrevealsntdnaja3responsestodroughtstress |