Protocol to characterize mouse dural mast cells by flow cytometry and immunofluorescence
Summary: Mast cells, which constitute tissue-resident immune cells, are distributed in the dural meninges. Here, we provide procedural guidelines for investigating mouse dural mast cells using two techniques. First, we outline the procedures for dural tissue dissection, single-cell isolation, and su...
Saved in:
| Main Authors: | Chih-Chung Jerry Lin, Nader Jaafar, Rudolph E. Tanzi |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672400529X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Protocol for assessing murine cell doublet engagement and subsequent effects using flow cytometry and imaging flow cytometry
by: Yuval Shapir Itai, et al.
Published: (2024-12-01) -
Protocol for cell proliferation and cell death analysis of primary muscle stem cell culture using flow cytometry
by: Pauline Garcia, et al.
Published: (2024-12-01) -
Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel
by: Pierre Lemaitre, et al.
Published: (2024-12-01) -
Protocol for intracellular immunofluorescence measurements of latent HIV reactivation in a primary CD4+ T cell model
by: Uri Mbonye, et al.
Published: (2024-12-01) -
Isolation and transcriptional profiling of antigen-presenting cells from mouse lung and MLNs following intranasal immunization
by: Xiuyu Wang, et al.
Published: (2024-12-01)