Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPK

Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPK

Redox reactions are essential for various biological processes, hence, maintaining redox homeostasis is critical for cellular health and host–microbe interactions, particularly in marine invertebrates, where these processes remain poorly understood. This study investigates the role of the respirator...

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Main Authors: Jiawei Cheng, Yunyu Shi, Yonghui Kong, Yuxi Wu, Yankang Wang, Jude Juventus Aweya, Shiyuan Bao, Junjie Nie, Qian Feng, Yongzhen Zhao, Yueling Zhang, Zhihong Zheng
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Virulence
Subjects:
Shrimp hemocyanin
redox balance
ROS
p38-MAPK-c-jun pathway
hemolymph Vibrio abundance
Online Access:https://www.tandfonline.com/doi/10.1080/21505594.2025.2546066
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Summary:Redox reactions are essential for various biological processes, hence, maintaining redox homeostasis is critical for cellular health and host–microbe interactions, particularly in marine invertebrates, where these processes remain poorly understood. This study investigates the role of the respiratory protein hemocyanin in regulating redox-related genes, reactive oxygen species (ROS) levels in shrimp hemocytes, and its influence on immune function and hemolymph Vibrio abundance. Our results show that hemocyanin is crucial in the regulation of the redox system, as hemocyanin knockdown disrupts the expression of antioxidant (PvPOD and PvGPX) and pro-oxidant (PvXOX) genes and redox enzyme activities, leading to elevated ROS levels in hemocytes. Additionally, we found that hemocyanin controls redox homeostasis in hemocytes through the p38-MAPK-c-Jun signaling pathway. Moreover, ROS levels regulated by hemocyanin via the MAPK pathway impacted on hemolymph Vibrio spp. abundance. These findings highlight a crucial role of hemocyanin as an antioxidant that modulates redox balance in shrimp hemocytes, with significant implications for immune defense and microbial homeostasis, offering new insights into antioxidative mechanisms in marine invertebrates.
ISSN:2150-5594
2150-5608

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