Comprehensive characterization and metabolomics to explore the role of hydrocortisone-induced yin deficiency syndrome in mice
Yin deficiency syndrome is a theoretical concept of traditional Chinese medicine (TCM) that claims an imbalance between Yin and Yang serves as a potential etiological factor disrupting physiological homeostasis. Its diagnosis in TCM is hindered by the intricate and diverse etiology, resulting in the...
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Elsevier
2024-12-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580824002322 |
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| author | Wenqi Jin Lan Yang Yuxin Zhang Yu Wang Yingna Li Yiming Zhao Liwei Sun Fangbing Liu |
| author_facet | Wenqi Jin Lan Yang Yuxin Zhang Yu Wang Yingna Li Yiming Zhao Liwei Sun Fangbing Liu |
| author_sort | Wenqi Jin |
| collection | DOAJ |
| description | Yin deficiency syndrome is a theoretical concept of traditional Chinese medicine (TCM) that claims an imbalance between Yin and Yang serves as a potential etiological factor disrupting physiological homeostasis. Its diagnosis in TCM is hindered by the intricate and diverse etiology, resulting in the absence of quantification and standardization. Hence, this study developed a hydrocortisone (HC) induced Yin deficiency syndrome model to investigate the intricate network underlying TCM and elucidate its intervention mechanism. In the findings, the model was characterized by weight loss, elevated drinking water, yellow urination, dry stools, variations in inflammatory factors, and higher levels of oxidative stress. Based on metabolomics, 56 metabolites showed different expressions; among them, 19 were upregulated, and 37 were downregulated. Bioinformatics analysis revealed that identified metabolites are mainly involved in glycerol phospholipid metabolism, pyrimidine metabolism, amino acid biosynthesis, arginine and proline metabolism, and arachidonic acid metabolism pathways. Finally, the metabolic network association confirmed the diagnostic accuracy of the Yin deficiency syndrome as established by HC. We propose for the first time an animal model of Yin deficiency syndrome induced by hydrocortisone in TCM clinical state. This research presents experimental evidence for establishing TCM symptoms and serves as a fundamental basis for the scientific awareness of their etiology. |
| format | Article |
| id | doaj-art-cbdc88e3ac004484b98168ebff4e8cff |
| institution | Kabale University |
| issn | 2405-5808 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-cbdc88e3ac004484b98168ebff4e8cff2024-12-04T05:13:10ZengElsevierBiochemistry and Biophysics Reports2405-58082024-12-0140101868Comprehensive characterization and metabolomics to explore the role of hydrocortisone-induced yin deficiency syndrome in miceWenqi Jin0Lan Yang1Yuxin Zhang2Yu Wang3Yingna Li4Yiming Zhao5Liwei Sun6Fangbing Liu7Research Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, ChinaResearch Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, ChinaResearch Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, ChinaCollege of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, ChinaResearch Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, ChinaResearch Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, ChinaResearch Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, China; Key Laboratory of Active Substances and Biological Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun, China; Corresponding author. Research Center of Traditional Chinese Medicine, the Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, China.Northeast Asian Institute of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, China; Corresponding author. Northeast Asian Institute of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, China.Yin deficiency syndrome is a theoretical concept of traditional Chinese medicine (TCM) that claims an imbalance between Yin and Yang serves as a potential etiological factor disrupting physiological homeostasis. Its diagnosis in TCM is hindered by the intricate and diverse etiology, resulting in the absence of quantification and standardization. Hence, this study developed a hydrocortisone (HC) induced Yin deficiency syndrome model to investigate the intricate network underlying TCM and elucidate its intervention mechanism. In the findings, the model was characterized by weight loss, elevated drinking water, yellow urination, dry stools, variations in inflammatory factors, and higher levels of oxidative stress. Based on metabolomics, 56 metabolites showed different expressions; among them, 19 were upregulated, and 37 were downregulated. Bioinformatics analysis revealed that identified metabolites are mainly involved in glycerol phospholipid metabolism, pyrimidine metabolism, amino acid biosynthesis, arginine and proline metabolism, and arachidonic acid metabolism pathways. Finally, the metabolic network association confirmed the diagnostic accuracy of the Yin deficiency syndrome as established by HC. We propose for the first time an animal model of Yin deficiency syndrome induced by hydrocortisone in TCM clinical state. This research presents experimental evidence for establishing TCM symptoms and serves as a fundamental basis for the scientific awareness of their etiology.http://www.sciencedirect.com/science/article/pii/S2405580824002322Traditional Chinese medicineYin deficiency syndromeHydrocortisoneMetabolomicsCharacterization |
| spellingShingle | Wenqi Jin Lan Yang Yuxin Zhang Yu Wang Yingna Li Yiming Zhao Liwei Sun Fangbing Liu Comprehensive characterization and metabolomics to explore the role of hydrocortisone-induced yin deficiency syndrome in mice Biochemistry and Biophysics Reports Traditional Chinese medicine Yin deficiency syndrome Hydrocortisone Metabolomics Characterization |
| title | Comprehensive characterization and metabolomics to explore the role of hydrocortisone-induced yin deficiency syndrome in mice |
| title_full | Comprehensive characterization and metabolomics to explore the role of hydrocortisone-induced yin deficiency syndrome in mice |
| title_fullStr | Comprehensive characterization and metabolomics to explore the role of hydrocortisone-induced yin deficiency syndrome in mice |
| title_full_unstemmed | Comprehensive characterization and metabolomics to explore the role of hydrocortisone-induced yin deficiency syndrome in mice |
| title_short | Comprehensive characterization and metabolomics to explore the role of hydrocortisone-induced yin deficiency syndrome in mice |
| title_sort | comprehensive characterization and metabolomics to explore the role of hydrocortisone induced yin deficiency syndrome in mice |
| topic | Traditional Chinese medicine Yin deficiency syndrome Hydrocortisone Metabolomics Characterization |
| url | http://www.sciencedirect.com/science/article/pii/S2405580824002322 |
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