Enhancement of tracheal cartilage regeneration by local controlled release of stromal cell-derived factor 1α with gelatin hydrogels and systemic administration of high-mobility group box 1 peptide
Introduction: This present study evaluated the effect of combination therapy with stromal cell-derived factor 1α (SDF-1α) and high-mobility group box 1 (HMGB1) peptide on the regeneration of tracheal injury in a rat model. Methods: To improve this effect, SDF-1α was incorporated into a gelatin hydro...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235232042400124X |
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| author | Kumiko (Ogi) Suzuki Tatsuya Okamoto Katsuto Tamai Yasuhiko Tabata Etsuro Hatano |
| author_facet | Kumiko (Ogi) Suzuki Tatsuya Okamoto Katsuto Tamai Yasuhiko Tabata Etsuro Hatano |
| author_sort | Kumiko (Ogi) Suzuki |
| collection | DOAJ |
| description | Introduction: This present study evaluated the effect of combination therapy with stromal cell-derived factor 1α (SDF-1α) and high-mobility group box 1 (HMGB1) peptide on the regeneration of tracheal injury in a rat model. Methods: To improve this effect, SDF-1α was incorporated into a gelatin hydrogel, which was then applied to the damaged tracheal cartilage of rats for local release. Furthermore, HMGB1 peptide was repeatedly administered intravenously. Regeneration of damaged tracheal cartilage was evaluated in terms of cell recruitment. Results: Mesenchymal stem cells (MSC) with C-X-C motif chemokine receptor 4 (CXCR4) were mobilized more into the injured area, and consequently the fastest tracheal cartilage regeneration was observed in the combination therapy group eight weeks after injury. Conclusions: The present study demonstrated that combination therapy with gelatin hydrogel incorporating SDF-1α and HMGB1 peptide injected intravenously can enhance the recruitment of CXCR4-positive MSC, promoting the regeneration of damaged tracheal cartilage. |
| format | Article |
| id | doaj-art-cb98c019f17d429cad951e90339feeb8 |
| institution | Kabale University |
| issn | 2352-3204 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-cb98c019f17d429cad951e90339feeb82024-11-30T07:10:51ZengElsevierRegenerative Therapy2352-32042024-06-0126415424Enhancement of tracheal cartilage regeneration by local controlled release of stromal cell-derived factor 1α with gelatin hydrogels and systemic administration of high-mobility group box 1 peptideKumiko (Ogi) Suzuki0Tatsuya Okamoto1Katsuto Tamai2Yasuhiko Tabata3Etsuro Hatano4Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan; Department of Biomaterials, Field of Tissue Engineering, Institute for Life and Medical Sciences, Kyoto University, Kyoto, JapanDepartment of Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanDepartment of Stem Cell Therapy Science, Graduate School of Medicine, Osaka University, JapanDepartment of Biomaterials, Field of Tissue Engineering, Institute for Life and Medical Sciences, Kyoto University, Kyoto, Japan; Corresponding author. Field of Tissue Engineering, Institute for Life and Medical Sciences, Kyoto University, Kyoto, Japan.Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanIntroduction: This present study evaluated the effect of combination therapy with stromal cell-derived factor 1α (SDF-1α) and high-mobility group box 1 (HMGB1) peptide on the regeneration of tracheal injury in a rat model. Methods: To improve this effect, SDF-1α was incorporated into a gelatin hydrogel, which was then applied to the damaged tracheal cartilage of rats for local release. Furthermore, HMGB1 peptide was repeatedly administered intravenously. Regeneration of damaged tracheal cartilage was evaluated in terms of cell recruitment. Results: Mesenchymal stem cells (MSC) with C-X-C motif chemokine receptor 4 (CXCR4) were mobilized more into the injured area, and consequently the fastest tracheal cartilage regeneration was observed in the combination therapy group eight weeks after injury. Conclusions: The present study demonstrated that combination therapy with gelatin hydrogel incorporating SDF-1α and HMGB1 peptide injected intravenously can enhance the recruitment of CXCR4-positive MSC, promoting the regeneration of damaged tracheal cartilage.http://www.sciencedirect.com/science/article/pii/S235232042400124XHigh-mobility group box 1 peptideMesenchymal stem cellStromal cell-derived factor-1αEndogenous regenerative therapyTracheobronchomalaciaGelatin hydrogel |
| spellingShingle | Kumiko (Ogi) Suzuki Tatsuya Okamoto Katsuto Tamai Yasuhiko Tabata Etsuro Hatano Enhancement of tracheal cartilage regeneration by local controlled release of stromal cell-derived factor 1α with gelatin hydrogels and systemic administration of high-mobility group box 1 peptide Regenerative Therapy High-mobility group box 1 peptide Mesenchymal stem cell Stromal cell-derived factor-1α Endogenous regenerative therapy Tracheobronchomalacia Gelatin hydrogel |
| title | Enhancement of tracheal cartilage regeneration by local controlled release of stromal cell-derived factor 1α with gelatin hydrogels and systemic administration of high-mobility group box 1 peptide |
| title_full | Enhancement of tracheal cartilage regeneration by local controlled release of stromal cell-derived factor 1α with gelatin hydrogels and systemic administration of high-mobility group box 1 peptide |
| title_fullStr | Enhancement of tracheal cartilage regeneration by local controlled release of stromal cell-derived factor 1α with gelatin hydrogels and systemic administration of high-mobility group box 1 peptide |
| title_full_unstemmed | Enhancement of tracheal cartilage regeneration by local controlled release of stromal cell-derived factor 1α with gelatin hydrogels and systemic administration of high-mobility group box 1 peptide |
| title_short | Enhancement of tracheal cartilage regeneration by local controlled release of stromal cell-derived factor 1α with gelatin hydrogels and systemic administration of high-mobility group box 1 peptide |
| title_sort | enhancement of tracheal cartilage regeneration by local controlled release of stromal cell derived factor 1α with gelatin hydrogels and systemic administration of high mobility group box 1 peptide |
| topic | High-mobility group box 1 peptide Mesenchymal stem cell Stromal cell-derived factor-1α Endogenous regenerative therapy Tracheobronchomalacia Gelatin hydrogel |
| url | http://www.sciencedirect.com/science/article/pii/S235232042400124X |
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