Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste
Abstract Oleaginous yeasts are considered promising sources for lipid production due to their ability to accumulate high levels of lipids under appropriate growth conditions. The current study aimed to isolate and identify oleaginous yeasts having superior ability to accumulate high quantities of li...
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BMC
2025-01-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-024-02635-3 |
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| author | AbdAllah M. Matouk Gadallah M. Abu-Elreesh Mohamed Ali Abdel-Rahman Said E. Desouky Amr H. Hashem |
| author_facet | AbdAllah M. Matouk Gadallah M. Abu-Elreesh Mohamed Ali Abdel-Rahman Said E. Desouky Amr H. Hashem |
| author_sort | AbdAllah M. Matouk |
| collection | DOAJ |
| description | Abstract Oleaginous yeasts are considered promising sources for lipid production due to their ability to accumulate high levels of lipids under appropriate growth conditions. The current study aimed to isolate and identify oleaginous yeasts having superior ability to accumulate high quantities of lipids; and enhancing lipid production using response surface methodology and repeated-batch fermentation. Results revealed that, twenty marine oleaginous yeasts were isolated, and the most potent lipid producer isolate was Candida parapsilosis Y19 according to qualitative screening test using Nile-red dye. Orange peels was used as substrate where C. parapsilosis Y19 produced 1.14 g/l lipids at 23.0% in batch fermentation. To enhance the lipid production, statistical optimization using Taguchi design through Response surface methodology was carried out. Total lipids were increased to 2.46 g/l and lipid content increased to 30.7% under optimal conditions of: orange peel 75 g/l, peptone 7 g/l, yeast extract 5 g/l, inoculum size 2% (v/v), pH 5 and incubation period 6 d. Furthermore, repeated-batch fermentation of C. parapsilosis Y19 enhanced lipid production where total lipids increased at 4.19 folds (4.78 g/l) compared to batch culture (before optimization). Also, the lipid content was increased at 1.7 folds (39.1%) compared to batch culture (before optimization). Fatty acid profile of the produced lipid using repeated-batch fermentation includes unsaturated fatty acids (USFAs) at 74.8% and saturated fatty acids (SFAs) at 25.1%. Additionally, in repeated-batch fermentation, the major fatty acid was oleic acid at 45.0%; followed by linoleic acid at 26.0%. In conclusion, C. parapsilosis Y19 is considered a promising strain for lipid production. Also, both statistical optimizations using RSM and repeated-batch fermentation are efficient methods for lipid production from C. parapsilosis Y19. |
| format | Article |
| id | doaj-art-cb978a4c957d4de8b88872c05b7a77e3 |
| institution | Kabale University |
| issn | 1475-2859 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-cb978a4c957d4de8b88872c05b7a77e32025-01-12T12:45:38ZengBMCMicrobial Cell Factories1475-28592025-01-0124111410.1186/s12934-024-02635-3Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel wasteAbdAllah M. Matouk0Gadallah M. Abu-Elreesh1Mohamed Ali Abdel-Rahman2Said E. Desouky3Amr H. Hashem4Botany and Microbiology Department , Faculty of Science, Al-Azhar UniversityEngineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City)Botany and Microbiology Department , Faculty of Science, Al-Azhar UniversityBotany and Microbiology Department , Faculty of Science, Al-Azhar UniversityBotany and Microbiology Department , Faculty of Science, Al-Azhar UniversityAbstract Oleaginous yeasts are considered promising sources for lipid production due to their ability to accumulate high levels of lipids under appropriate growth conditions. The current study aimed to isolate and identify oleaginous yeasts having superior ability to accumulate high quantities of lipids; and enhancing lipid production using response surface methodology and repeated-batch fermentation. Results revealed that, twenty marine oleaginous yeasts were isolated, and the most potent lipid producer isolate was Candida parapsilosis Y19 according to qualitative screening test using Nile-red dye. Orange peels was used as substrate where C. parapsilosis Y19 produced 1.14 g/l lipids at 23.0% in batch fermentation. To enhance the lipid production, statistical optimization using Taguchi design through Response surface methodology was carried out. Total lipids were increased to 2.46 g/l and lipid content increased to 30.7% under optimal conditions of: orange peel 75 g/l, peptone 7 g/l, yeast extract 5 g/l, inoculum size 2% (v/v), pH 5 and incubation period 6 d. Furthermore, repeated-batch fermentation of C. parapsilosis Y19 enhanced lipid production where total lipids increased at 4.19 folds (4.78 g/l) compared to batch culture (before optimization). Also, the lipid content was increased at 1.7 folds (39.1%) compared to batch culture (before optimization). Fatty acid profile of the produced lipid using repeated-batch fermentation includes unsaturated fatty acids (USFAs) at 74.8% and saturated fatty acids (SFAs) at 25.1%. Additionally, in repeated-batch fermentation, the major fatty acid was oleic acid at 45.0%; followed by linoleic acid at 26.0%. In conclusion, C. parapsilosis Y19 is considered a promising strain for lipid production. Also, both statistical optimizations using RSM and repeated-batch fermentation are efficient methods for lipid production from C. parapsilosis Y19.https://doi.org/10.1186/s12934-024-02635-3Oleaginous yeastOrange peels lipid productionTaguchi designRepeated batch fermentationCandida parapsilosis |
| spellingShingle | AbdAllah M. Matouk Gadallah M. Abu-Elreesh Mohamed Ali Abdel-Rahman Said E. Desouky Amr H. Hashem Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste Microbial Cell Factories Oleaginous yeast Orange peels lipid production Taguchi design Repeated batch fermentation Candida parapsilosis |
| title | Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste |
| title_full | Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste |
| title_fullStr | Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste |
| title_full_unstemmed | Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste |
| title_short | Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste |
| title_sort | response surface methodology and repeated batch fermentation strategies for enhancing lipid production from marine oleaginous candida parapsilosis y19 using orange peel waste |
| topic | Oleaginous yeast Orange peels lipid production Taguchi design Repeated batch fermentation Candida parapsilosis |
| url | https://doi.org/10.1186/s12934-024-02635-3 |
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