Elevated circulating TLR4+ monocytes in patients with liver fluke Opisthorchis viverrini is associated with advanced periductal fibrosis
Introduction: Opisthorchis viverrini (Ov) infection can lead to several disease manifestations of the bile duct including advanced periductal fibrosis (APF) and the most severe complication, cholangiocarcinoma (CCA). Monocytes migrate to the infection site and differentiate into tissue macrophages...
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The Journal of Infection in Developing Countries
2023-08-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/17711 |
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| author | Kanin Salao Chakrit Thapphan Tran Duong Thai Kiatichai Faksri Sutas Suttiprapa Sirikachorn Tangkawattana Banchob Sripa B Steven W Edwards |
| author_facet | Kanin Salao Chakrit Thapphan Tran Duong Thai Kiatichai Faksri Sutas Suttiprapa Sirikachorn Tangkawattana Banchob Sripa B Steven W Edwards |
| author_sort | Kanin Salao |
| collection | DOAJ |
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Introduction: Opisthorchis viverrini (Ov) infection can lead to several disease manifestations of the bile duct including advanced periductal fibrosis (APF) and the most severe complication, cholangiocarcinoma (CCA). Monocytes migrate to the infection site and differentiate into tissue macrophages to express and release molecules such as cytokines, reactive oxygen species, and growth factors. TLR4+ monocytes are classified as having a pro-tumor phenotype and secrete tumor-promoting factors. The aim of this study is to investigate the role of monocytes in the pathogenesis of opisthorchiasis.
Methodology: We used flow cytometry to measure the number of TLR4+ monocytes in the circulating blood of Ov infected patients with or without APF compared to healthy, non-Ov-infected controls.
Results: We found, for the first time, that patients with AFP have elevated numbers of circulating TLR4+ monocytes when compared to patients without fibrosis and healthy individuals. Intriguingly, when we measured ROS from these monocytes, we found increased ROS production in patients with APF.
Conclusions: We propose that excessive production of ROS from these TLR4+ monocytes may lead to excessive injury of surrounding tissue and hence contribute to the pathological processes that lead to the development of advanced periductal fibrosis.
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| format | Article |
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| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2023-08-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-cb8e6c2f8f0041ccb074653af8f8076d2025-08-20T03:52:38ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802023-08-01170810.3855/jidc.17711Elevated circulating TLR4+ monocytes in patients with liver fluke Opisthorchis viverrini is associated with advanced periductal fibrosisKanin Salao0Chakrit Thapphan1Tran Duong Thai2Kiatichai Faksri3Sutas Suttiprapa4Sirikachorn Tangkawattana5Banchob Sripa B6Steven W Edwards7Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandWHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver fluke Disease), Tropical Disease Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandWHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver fluke Disease), Tropical Disease Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandWHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver fluke Disease), Tropical Disease Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandInstitute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom Introduction: Opisthorchis viverrini (Ov) infection can lead to several disease manifestations of the bile duct including advanced periductal fibrosis (APF) and the most severe complication, cholangiocarcinoma (CCA). Monocytes migrate to the infection site and differentiate into tissue macrophages to express and release molecules such as cytokines, reactive oxygen species, and growth factors. TLR4+ monocytes are classified as having a pro-tumor phenotype and secrete tumor-promoting factors. The aim of this study is to investigate the role of monocytes in the pathogenesis of opisthorchiasis. Methodology: We used flow cytometry to measure the number of TLR4+ monocytes in the circulating blood of Ov infected patients with or without APF compared to healthy, non-Ov-infected controls. Results: We found, for the first time, that patients with AFP have elevated numbers of circulating TLR4+ monocytes when compared to patients without fibrosis and healthy individuals. Intriguingly, when we measured ROS from these monocytes, we found increased ROS production in patients with APF. Conclusions: We propose that excessive production of ROS from these TLR4+ monocytes may lead to excessive injury of surrounding tissue and hence contribute to the pathological processes that lead to the development of advanced periductal fibrosis. https://jidc.org/index.php/journal/article/view/17711monocytesOpisthorchis viverrinetoll-like receptor 4reactive oxygen speciesadvanced periductal fibrosis |
| spellingShingle | Kanin Salao Chakrit Thapphan Tran Duong Thai Kiatichai Faksri Sutas Suttiprapa Sirikachorn Tangkawattana Banchob Sripa B Steven W Edwards Elevated circulating TLR4+ monocytes in patients with liver fluke Opisthorchis viverrini is associated with advanced periductal fibrosis Journal of Infection in Developing Countries monocytes Opisthorchis viverrine toll-like receptor 4 reactive oxygen species advanced periductal fibrosis |
| title | Elevated circulating TLR4+ monocytes in patients with liver fluke Opisthorchis viverrini is associated with advanced periductal fibrosis |
| title_full | Elevated circulating TLR4+ monocytes in patients with liver fluke Opisthorchis viverrini is associated with advanced periductal fibrosis |
| title_fullStr | Elevated circulating TLR4+ monocytes in patients with liver fluke Opisthorchis viverrini is associated with advanced periductal fibrosis |
| title_full_unstemmed | Elevated circulating TLR4+ monocytes in patients with liver fluke Opisthorchis viverrini is associated with advanced periductal fibrosis |
| title_short | Elevated circulating TLR4+ monocytes in patients with liver fluke Opisthorchis viverrini is associated with advanced periductal fibrosis |
| title_sort | elevated circulating tlr4 monocytes in patients with liver fluke opisthorchis viverrini is associated with advanced periductal fibrosis |
| topic | monocytes Opisthorchis viverrine toll-like receptor 4 reactive oxygen species advanced periductal fibrosis |
| url | https://jidc.org/index.php/journal/article/view/17711 |
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