Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions

BackgroundMosquito-borne diseases have a significant public health threat worldwide, with arboviruses accounting for a high proportion of infectious diseases and mortality annually. Brazil, in particular, has been suffering outbreaks of diseases transmitted by mosquito viruses, notably those of the...

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Main Authors: Lilian de Oliveira Guimarães, Geovani de Oliveira Ribeiro, Roseane da Couto, Endrya do Socorro Foro Ramos, Vanessa dos Santos Morais, Juliana Telles-de-Deus, Vanessa Christe Helfstein, Jesus Maia dos Santos, Xutao Deng, Eric Delwart, Ramendra Pati Pandey, Vera Lucia Fonseca de Camargo-Neves, Antonio Charlys da Costa, Karin Kirchgatter, Élcio Leal
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Cellular and Infection Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2024.1496126/full
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author Lilian de Oliveira Guimarães
Geovani de Oliveira Ribeiro
Geovani de Oliveira Ribeiro
Roseane da Couto
Endrya do Socorro Foro Ramos
Vanessa dos Santos Morais
Juliana Telles-de-Deus
Vanessa Christe Helfstein
Jesus Maia dos Santos
Xutao Deng
Xutao Deng
Eric Delwart
Ramendra Pati Pandey
Vera Lucia Fonseca de Camargo-Neves
Antonio Charlys da Costa
Karin Kirchgatter
Karin Kirchgatter
Élcio Leal
author_facet Lilian de Oliveira Guimarães
Geovani de Oliveira Ribeiro
Geovani de Oliveira Ribeiro
Roseane da Couto
Endrya do Socorro Foro Ramos
Vanessa dos Santos Morais
Juliana Telles-de-Deus
Vanessa Christe Helfstein
Jesus Maia dos Santos
Xutao Deng
Xutao Deng
Eric Delwart
Ramendra Pati Pandey
Vera Lucia Fonseca de Camargo-Neves
Antonio Charlys da Costa
Karin Kirchgatter
Karin Kirchgatter
Élcio Leal
author_sort Lilian de Oliveira Guimarães
collection DOAJ
description BackgroundMosquito-borne diseases have a significant public health threat worldwide, with arboviruses accounting for a high proportion of infectious diseases and mortality annually. Brazil, in particular, has been suffering outbreaks of diseases transmitted by mosquito viruses, notably those of the Aedes genus, such as dengue, Zika, and chikungunya. Against this background, the São Paulo Zoo is an intriguing ecological niche to explore the virome of mosquitoes, potentially shedding light on the dynamics of arbovirus transmission within a confined setting.MethodsIn this study, we conducted a comprehensive metagenomic analysis of mosquitoes collected from diverse habitats within the zoo, focusing on the Aedes, Anopheles, and Culex genera. From 1,039 contigs of viral origin, we identified 229 viral species infecting mosquitoes, with the orders Picornavirales, Nodamuvirales and Sobelivirales being the most prevalent and abundant. The difference in virome composition was primarily driven by mosquito host species rather than specific collection sites or trap height.ResultsDespite environmental disparities, the virome remained remarkably uniform across different areas of the zoo, emphasizing the strong association between mosquito species and their viral communities. Furthermore, we identified a core virome shared among mosquito species, highlighting potential cross-species transmission events and underscoring the need for targeted surveillance and control measures.ConclusionThese results contribute to our understanding of the interplay between mosquitoes, the environment, and viruses, providing valuable insights for disease intervention strategies in mosquito-borne diseases.
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spelling doaj-art-cb5990e5523548e2abf85e48d312231f2025-01-10T06:10:49ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.14961261496126Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactionsLilian de Oliveira Guimarães0Geovani de Oliveira Ribeiro1Geovani de Oliveira Ribeiro2Roseane da Couto3Endrya do Socorro Foro Ramos4Vanessa dos Santos Morais5Juliana Telles-de-Deus6Vanessa Christe Helfstein7Jesus Maia dos Santos8Xutao Deng9Xutao Deng10Eric Delwart11Ramendra Pati Pandey12Vera Lucia Fonseca de Camargo-Neves13Antonio Charlys da Costa14Karin Kirchgatter15Karin Kirchgatter16Élcio Leal17Instituto Pasteur, São Paulo, SP, BrazilGeneral-Coordination of Public Health Laboratories, Health and Environment Surveillance Secretariat, Ministry of Health, Brasilia, BrazilDepartment of Cellular Biology, University of Brasilia (UNB), Brasilia, BrazilInstitute of Biological Sciences, Federal University of Pará, Belem, Pará, BrazilInstitute of Biological Sciences, Federal University of Pará, Belem, Pará, BrazilInstituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BrazilInstituto Pasteur, São Paulo, SP, BrazilInstituto Pasteur, São Paulo, SP, BrazilInstituto Pasteur, São Paulo, SP, BrazilVitalant Research Institute, San Francisco, CA, United StatesDepartment Laboratory Medicine, University of California, San Francisco, San Francisco, CA, United StatesDepartment Laboratory Medicine, University of California, San Francisco, San Francisco, CA, United StatesSchool of Health Sciences and Technology (SoHST), University of Petroleum and Energy Studies (UPES), Dehradun, Uttarakhand, IndiaInstituto Pasteur, São Paulo, SP, BrazilInstituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BrazilInstituto Pasteur, São Paulo, SP, BrazilInstituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BrazilInstitute of Biological Sciences, Federal University of Pará, Belem, Pará, BrazilBackgroundMosquito-borne diseases have a significant public health threat worldwide, with arboviruses accounting for a high proportion of infectious diseases and mortality annually. Brazil, in particular, has been suffering outbreaks of diseases transmitted by mosquito viruses, notably those of the Aedes genus, such as dengue, Zika, and chikungunya. Against this background, the São Paulo Zoo is an intriguing ecological niche to explore the virome of mosquitoes, potentially shedding light on the dynamics of arbovirus transmission within a confined setting.MethodsIn this study, we conducted a comprehensive metagenomic analysis of mosquitoes collected from diverse habitats within the zoo, focusing on the Aedes, Anopheles, and Culex genera. From 1,039 contigs of viral origin, we identified 229 viral species infecting mosquitoes, with the orders Picornavirales, Nodamuvirales and Sobelivirales being the most prevalent and abundant. The difference in virome composition was primarily driven by mosquito host species rather than specific collection sites or trap height.ResultsDespite environmental disparities, the virome remained remarkably uniform across different areas of the zoo, emphasizing the strong association between mosquito species and their viral communities. Furthermore, we identified a core virome shared among mosquito species, highlighting potential cross-species transmission events and underscoring the need for targeted surveillance and control measures.ConclusionThese results contribute to our understanding of the interplay between mosquitoes, the environment, and viruses, providing valuable insights for disease intervention strategies in mosquito-borne diseases.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1496126/fullmetagenomicsviromemosquitoesSão Paulo Zoovector-borne arbovirus
spellingShingle Lilian de Oliveira Guimarães
Geovani de Oliveira Ribeiro
Geovani de Oliveira Ribeiro
Roseane da Couto
Endrya do Socorro Foro Ramos
Vanessa dos Santos Morais
Juliana Telles-de-Deus
Vanessa Christe Helfstein
Jesus Maia dos Santos
Xutao Deng
Xutao Deng
Eric Delwart
Ramendra Pati Pandey
Vera Lucia Fonseca de Camargo-Neves
Antonio Charlys da Costa
Karin Kirchgatter
Karin Kirchgatter
Élcio Leal
Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions
Frontiers in Cellular and Infection Microbiology
metagenomics
virome
mosquitoes
São Paulo Zoo
vector-borne arbovirus
title Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions
title_full Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions
title_fullStr Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions
title_full_unstemmed Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions
title_short Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions
title_sort exploring mosquito virome dynamics within sao paulo zoo insights into mosquito virus environment interactions
topic metagenomics
virome
mosquitoes
São Paulo Zoo
vector-borne arbovirus
url https://www.frontiersin.org/articles/10.3389/fcimb.2024.1496126/full
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