The Effect of Conditioned Medium from Angiopoietin-1 Gene-Modified Mesenchymal Stem Cells on Wound Healing in a Diabetic Mouse Model
Introduction: Mesenchymal stem cells (MSCs) have been introduced as a promising treatment for diabetic wounds. The effects of stem cell therapy are thought to be caused by bioactive molecules secreted by stem cells. Stem cell-based gene therapies can target bioactive molecules. Therefore, treatment...
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2024-12-01
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| author | Qiong Deng Shenzhen Pan Fangzhou Du Hongfei Sang Zhixin Cai Xiaoyu Xu Qian Wei Shuang Yu Jingzhong Zhang Chenglong Li |
| author_facet | Qiong Deng Shenzhen Pan Fangzhou Du Hongfei Sang Zhixin Cai Xiaoyu Xu Qian Wei Shuang Yu Jingzhong Zhang Chenglong Li |
| author_sort | Qiong Deng |
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| description | Introduction: Mesenchymal stem cells (MSCs) have been introduced as a promising treatment for diabetic wounds. The effects of stem cell therapy are thought to be caused by bioactive molecules secreted by stem cells. Stem cell-based gene therapies can target bioactive molecules. Therefore, treatment using conditioned medium (CM) derived from genetically engineered stem cells has been proposed as an alternative option for diabetic ulcer care. Methods: MSCs derived from human umbilical cords were obtained and engineered to overexpress the angiogenin-1 gene (MSCs<sup>Ang1</sup>) through plasmid transfection. This study extracted conditioned medium from MSCs (MSC-CM) or MSCs<sup>Ang1</sup>(MSC<sup>Ang1</sup>-CM) for wound treatment applications. Via in vitro experiments, the proangiogenic effects of MSC<sup>Ang1</sup>-CM were assessed via the migration and tube formation of human umbilical vein endothelial cells (HUVECs). Furthermore, the efficacy of MSC<sup>Ang1</sup>-CM in promoting wound healing, re-epithelialization, hair follicle, and angiogenesis was evaluated via a diabetic mouse skin defect model. Results: In vitro assays demonstrated that MSC<sup>Ang1</sup>-CM significantly enhanced HUVECs’ functions, including migration and tube formation. In vivo assays revealed that MSC<sup>Ang1</sup>-CM exhibited notable advancements in healing speed, re-epithelialization, hair follicle, and angiogenesis. Conclusion: These results indicate that MSC<sup>Ang1</sup>-CM can promote wound healing in diabetic mice and make the vascular structure in regenerated tissues more stable without inducing tissue fibrosis, providing a new therapeutic strategy for treating diabetic skin wounds. This provides a valuable theoretical basis for further research on regenerative medicine and cell therapy. |
| format | Article |
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| language | English |
| publishDate | 2024-12-01 |
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| series | Bioengineering |
| spelling | doaj-art-cb0681010f2d4d2499525f3d5041d4b52024-12-27T14:11:36ZengMDPI AGBioengineering2306-53542024-12-011112124410.3390/bioengineering11121244The Effect of Conditioned Medium from Angiopoietin-1 Gene-Modified Mesenchymal Stem Cells on Wound Healing in a Diabetic Mouse ModelQiong Deng0Shenzhen Pan1Fangzhou Du2Hongfei Sang3Zhixin Cai4Xiaoyu Xu5Qian Wei6Shuang Yu7Jingzhong Zhang8Chenglong Li9Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, ChinaDepartment of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, ChinaSchool of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, ChinaDepartment of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, ChinaDepartment of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, ChinaDepartment of Radiology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441000, ChinaSchool of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, ChinaSchool of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, ChinaSchool of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, ChinaDepartment of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, ChinaIntroduction: Mesenchymal stem cells (MSCs) have been introduced as a promising treatment for diabetic wounds. The effects of stem cell therapy are thought to be caused by bioactive molecules secreted by stem cells. Stem cell-based gene therapies can target bioactive molecules. Therefore, treatment using conditioned medium (CM) derived from genetically engineered stem cells has been proposed as an alternative option for diabetic ulcer care. Methods: MSCs derived from human umbilical cords were obtained and engineered to overexpress the angiogenin-1 gene (MSCs<sup>Ang1</sup>) through plasmid transfection. This study extracted conditioned medium from MSCs (MSC-CM) or MSCs<sup>Ang1</sup>(MSC<sup>Ang1</sup>-CM) for wound treatment applications. Via in vitro experiments, the proangiogenic effects of MSC<sup>Ang1</sup>-CM were assessed via the migration and tube formation of human umbilical vein endothelial cells (HUVECs). Furthermore, the efficacy of MSC<sup>Ang1</sup>-CM in promoting wound healing, re-epithelialization, hair follicle, and angiogenesis was evaluated via a diabetic mouse skin defect model. Results: In vitro assays demonstrated that MSC<sup>Ang1</sup>-CM significantly enhanced HUVECs’ functions, including migration and tube formation. In vivo assays revealed that MSC<sup>Ang1</sup>-CM exhibited notable advancements in healing speed, re-epithelialization, hair follicle, and angiogenesis. Conclusion: These results indicate that MSC<sup>Ang1</sup>-CM can promote wound healing in diabetic mice and make the vascular structure in regenerated tissues more stable without inducing tissue fibrosis, providing a new therapeutic strategy for treating diabetic skin wounds. This provides a valuable theoretical basis for further research on regenerative medicine and cell therapy.https://www.mdpi.com/2306-5354/11/12/1244mesenchymal stem cellconditioned mediumAngiopoietin 1diabetic wound healingangiogenesis |
| spellingShingle | Qiong Deng Shenzhen Pan Fangzhou Du Hongfei Sang Zhixin Cai Xiaoyu Xu Qian Wei Shuang Yu Jingzhong Zhang Chenglong Li The Effect of Conditioned Medium from Angiopoietin-1 Gene-Modified Mesenchymal Stem Cells on Wound Healing in a Diabetic Mouse Model Bioengineering mesenchymal stem cell conditioned medium Angiopoietin 1 diabetic wound healing angiogenesis |
| title | The Effect of Conditioned Medium from Angiopoietin-1 Gene-Modified Mesenchymal Stem Cells on Wound Healing in a Diabetic Mouse Model |
| title_full | The Effect of Conditioned Medium from Angiopoietin-1 Gene-Modified Mesenchymal Stem Cells on Wound Healing in a Diabetic Mouse Model |
| title_fullStr | The Effect of Conditioned Medium from Angiopoietin-1 Gene-Modified Mesenchymal Stem Cells on Wound Healing in a Diabetic Mouse Model |
| title_full_unstemmed | The Effect of Conditioned Medium from Angiopoietin-1 Gene-Modified Mesenchymal Stem Cells on Wound Healing in a Diabetic Mouse Model |
| title_short | The Effect of Conditioned Medium from Angiopoietin-1 Gene-Modified Mesenchymal Stem Cells on Wound Healing in a Diabetic Mouse Model |
| title_sort | effect of conditioned medium from angiopoietin 1 gene modified mesenchymal stem cells on wound healing in a diabetic mouse model |
| topic | mesenchymal stem cell conditioned medium Angiopoietin 1 diabetic wound healing angiogenesis |
| url | https://www.mdpi.com/2306-5354/11/12/1244 |
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