Cytotoxic Flavonoids from Lannea egregia Engl. & K. Krause
Background: Lannea egregia Engl. & K. Krause (family: Anacardiaceae) is a well-known medicinal plant in Nigeria whose various parts have been shown to elicit several biological activities. This study specifically explored the leaf of L. egregia for potential cytotoxic compounds. Methods: n-Hexan...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2024-10-01
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| Series: | Pharmaceutical Sciences |
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| Online Access: | https://ps.tbzmed.ac.ir/PDF/ps-30-496.pdf |
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| author | Oluwatosin T. Ayodele Olaoluwa O. Olaoluwa Saheed O. Benson Olapeju O. Aiyelaagbe Lutfun Nahar Amos A. Fatokun Satyajit D. Sarker |
| author_facet | Oluwatosin T. Ayodele Olaoluwa O. Olaoluwa Saheed O. Benson Olapeju O. Aiyelaagbe Lutfun Nahar Amos A. Fatokun Satyajit D. Sarker |
| author_sort | Oluwatosin T. Ayodele |
| collection | DOAJ |
| description | Background: Lannea egregia Engl. & K. Krause (family: Anacardiaceae) is a well-known medicinal plant in Nigeria whose various parts have been shown to elicit several biological activities. This study specifically explored the leaf of L. egregia for potential cytotoxic compounds. Methods: n-Hexane, dichloromethane and methanolic extracts of the leaf were prepared using the Soxhlet apparatus and concentrated using the rotary evaporator. Compounds were isolated by reversed-phase preparative high-performance liquid chromatography, and the structures were determined by spectroscopic means. The methanolic extract and the isolated compounds were screened for cytotoxicity against HeLa and MCF-7 cancer cell lines, using the MTT assay. Results: Three flavonoids, myricetin (1), myricetin 3-O-α-L-rhamnoside (2) and quercetin 3-O-α-L-rhamnoside (3), were isolated from the methanolic extract of the leaf of L. egregia. The methanolic extract and compound 3 showed the most potent inhibition profiles against the cells, with IC50 values (Mean ± SEM) of 45.3 ± 1.5 µg/mL and 57.5 ± 0.4 µg/mL for the methanolic extract, and 36.5 ± 2.0 µM and 57.9 ± 10.1 µM for compound 3, against HeLa and MCF-7 cells, respectively. Conclusion: Conclusion: This work shows that L. egregia leaf is moderately cytotoxic, and rich in flavonoids, and the cytotoxicity of the extract is, at least partially, due to the presence of cytotoxic flavonoids. This is the first report on characterized isolated compounds from the leaf of L. egregia and the occurrence of flavonols in the leaf. |
| format | Article |
| id | doaj-art-cadf4a2b876845b994bdb63e1e63c097 |
| institution | Kabale University |
| issn | 2383-2886 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical Sciences |
| spelling | doaj-art-cadf4a2b876845b994bdb63e1e63c0972024-11-18T11:45:05ZengTabriz University of Medical SciencesPharmaceutical Sciences2383-28862024-10-0130449650110.34172/PS.2024.26ps-40390Cytotoxic Flavonoids from Lannea egregia Engl. & K. KrauseOluwatosin T. Ayodele0Olaoluwa O. Olaoluwa1Saheed O. Benson2Olapeju O. Aiyelaagbe3Lutfun Nahar4Amos A. Fatokun5Satyajit D. Sarker6Department of Chemistry, University of Ibadan, Ibadan, Oyo State, 200284, Nigeria.Department of Chemistry, University of Ibadan, Ibadan, Oyo State, 200284, Nigeria.Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, United Kingdom.Department of Chemistry, University of Ibadan, Ibadan, Oyo State, 200284, Nigeria.Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany, The Czech Academy of Sciences, Šlechtitelů 27, 78371 Olomouc, Czech Republic.Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, United Kingdom.Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, United Kingdom.Background: Lannea egregia Engl. & K. Krause (family: Anacardiaceae) is a well-known medicinal plant in Nigeria whose various parts have been shown to elicit several biological activities. This study specifically explored the leaf of L. egregia for potential cytotoxic compounds. Methods: n-Hexane, dichloromethane and methanolic extracts of the leaf were prepared using the Soxhlet apparatus and concentrated using the rotary evaporator. Compounds were isolated by reversed-phase preparative high-performance liquid chromatography, and the structures were determined by spectroscopic means. The methanolic extract and the isolated compounds were screened for cytotoxicity against HeLa and MCF-7 cancer cell lines, using the MTT assay. Results: Three flavonoids, myricetin (1), myricetin 3-O-α-L-rhamnoside (2) and quercetin 3-O-α-L-rhamnoside (3), were isolated from the methanolic extract of the leaf of L. egregia. The methanolic extract and compound 3 showed the most potent inhibition profiles against the cells, with IC50 values (Mean ± SEM) of 45.3 ± 1.5 µg/mL and 57.5 ± 0.4 µg/mL for the methanolic extract, and 36.5 ± 2.0 µM and 57.9 ± 10.1 µM for compound 3, against HeLa and MCF-7 cells, respectively. Conclusion: Conclusion: This work shows that L. egregia leaf is moderately cytotoxic, and rich in flavonoids, and the cytotoxicity of the extract is, at least partially, due to the presence of cytotoxic flavonoids. This is the first report on characterized isolated compounds from the leaf of L. egregia and the occurrence of flavonols in the leaf.https://ps.tbzmed.ac.ir/PDF/ps-30-496.pdfanacardiaceaecancer cell linescytotoxicityflavonoidslannea egregiamyricetin |
| spellingShingle | Oluwatosin T. Ayodele Olaoluwa O. Olaoluwa Saheed O. Benson Olapeju O. Aiyelaagbe Lutfun Nahar Amos A. Fatokun Satyajit D. Sarker Cytotoxic Flavonoids from Lannea egregia Engl. & K. Krause Pharmaceutical Sciences anacardiaceae cancer cell lines cytotoxicity flavonoids lannea egregia myricetin |
| title | Cytotoxic Flavonoids from Lannea egregia Engl. & K. Krause |
| title_full | Cytotoxic Flavonoids from Lannea egregia Engl. & K. Krause |
| title_fullStr | Cytotoxic Flavonoids from Lannea egregia Engl. & K. Krause |
| title_full_unstemmed | Cytotoxic Flavonoids from Lannea egregia Engl. & K. Krause |
| title_short | Cytotoxic Flavonoids from Lannea egregia Engl. & K. Krause |
| title_sort | cytotoxic flavonoids from lannea egregia engl k krause |
| topic | anacardiaceae cancer cell lines cytotoxicity flavonoids lannea egregia myricetin |
| url | https://ps.tbzmed.ac.ir/PDF/ps-30-496.pdf |
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