Effect of <i>Lactobacillus paracasei</i> LK01 on Growth Performance, Antioxidant Capacity, Immunity, Intestinal Health, and Serum Biochemical Indices in Broilers
This study aimed to investigate the effects of adding <i>L. paracasei</i> LK01 to the diet on the growth performance, antioxidant capacity, immunity, intestinal health, and serum biochemical indicators of broilers. This study selected 1080 one-day-old broiler chickens with similar body w...
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2024-12-01
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| author | Weixin Liu Hong Cheng Hao Zhang Guozhen Liu Xinyu Yin Cheng Zhang Runsheng Jiang Zaigui Wang Xiaoling Ding |
| author_facet | Weixin Liu Hong Cheng Hao Zhang Guozhen Liu Xinyu Yin Cheng Zhang Runsheng Jiang Zaigui Wang Xiaoling Ding |
| author_sort | Weixin Liu |
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| description | This study aimed to investigate the effects of adding <i>L. paracasei</i> LK01 to the diet on the growth performance, antioxidant capacity, immunity, intestinal health, and serum biochemical indicators of broilers. This study selected 1080 one-day-old broiler chickens with similar body weight, and randomly divided them into six groups, with six replicates in each group and 30 chicks in each replicate. The chicks were fed (1) the basal diet (CON), (2) the basal diet with 10<sup>6</sup> CFU/kg <i>L. paracasei</i> LK01(T1), (3) the basal diet with 10<sup>7</sup> CFU/kg <i>L. paracasei</i> LK01(T2), (4) the basal diet with 10<sup>8</sup> CFU/kg <i>L. paracasei</i> LK01(T3), (5) the basal diet with 10<sup>9</sup> CFU/kg <i>L. paracasei</i> LK01(T4), and (6) the basal diet with 10<sup>10</sup> CFU/kg <i>L. paracasei</i> LK01(T5). The experiment lasted for 42 days. In this study, compared with the CON group, the diet supplemented with <i>L. paracasei</i> significantly increased body weight from 1 to 21 days (<i>p</i> < 0.05). In addition, the 10<sup>6</sup> CFU/kg <i>L. paracasei</i> LK01 group significantly reduced the activity of glutamic oxaloacetic transaminase and triglyceride levels; the 10<sup>7</sup> CFU/kg,10<sup>8</sup> CFU/kg, and 10<sup>9</sup> CFU/kg <i>L. paracasei</i> LK01 groups also reduced serum uric acid and total cholesterol levels (<i>p</i> < 0.05). The experimental groups all had lower serum levels of malondialdehyde and interleukin-1β (<i>p</i> < 0.01). Except for the 10<sup>6</sup> CFU/kg group, all experimental groups had significantly lower tumor necrosis factor-α, and the 10<sup>6</sup> and 10<sup>7</sup> CFU/kg groups had higher immunoglobulin M levels (<i>p</i> < 0.05). In addition, the 10<sup>6</sup> CFU/kg group significantly reduced the depth of the ileocecal crypts and increased the villus-to-crypt ratio (V/C) of the jejunum and ileum. In addition, dietary supplementation with <i>L. paracasei</i> LK01 did not change the α diversity of the microbial community in the cecum, but significantly increased the proportion of <i>Bacteroides</i> (phylum) (<i>p</i> < 0.05). The 10<sup>6</sup> CFU/kg group also significantly increased the abundance of beneficial bacteria such as <i>Ruminococcaceae</i> (genus), <i>Lachnospiraceae</i> (genus), and <i>Faecalibacterium</i> (genus) (<i>p</i> < 0.05). In summary, this study revealed that adding 10<sup>6</sup> CFU/kg of <i>L. paracasei</i> LKO1 to broiler diets can improve their production performance, serum biochemical indicators, antioxidant, and immune capabilities, as well as cecal flora. |
| format | Article |
| id | doaj-art-cadf2a59a12549c5af89f51cf809f287 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-cadf2a59a12549c5af89f51cf809f2872024-12-13T16:21:21ZengMDPI AGAnimals2076-26152024-12-011423347410.3390/ani14233474Effect of <i>Lactobacillus paracasei</i> LK01 on Growth Performance, Antioxidant Capacity, Immunity, Intestinal Health, and Serum Biochemical Indices in BroilersWeixin Liu0Hong Cheng1Hao Zhang2Guozhen Liu3Xinyu Yin4Cheng Zhang5Runsheng Jiang6Zaigui Wang7Xiaoling Ding8College of Animal Science and Technology, Anhui Agricultural University, Hefei 230031, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230031, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230031, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230031, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230031, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230031, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230031, ChinaCollege of Life Science and Technology, Anhui Agricultural University, Hefei 230031, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230031, ChinaThis study aimed to investigate the effects of adding <i>L. paracasei</i> LK01 to the diet on the growth performance, antioxidant capacity, immunity, intestinal health, and serum biochemical indicators of broilers. This study selected 1080 one-day-old broiler chickens with similar body weight, and randomly divided them into six groups, with six replicates in each group and 30 chicks in each replicate. The chicks were fed (1) the basal diet (CON), (2) the basal diet with 10<sup>6</sup> CFU/kg <i>L. paracasei</i> LK01(T1), (3) the basal diet with 10<sup>7</sup> CFU/kg <i>L. paracasei</i> LK01(T2), (4) the basal diet with 10<sup>8</sup> CFU/kg <i>L. paracasei</i> LK01(T3), (5) the basal diet with 10<sup>9</sup> CFU/kg <i>L. paracasei</i> LK01(T4), and (6) the basal diet with 10<sup>10</sup> CFU/kg <i>L. paracasei</i> LK01(T5). The experiment lasted for 42 days. In this study, compared with the CON group, the diet supplemented with <i>L. paracasei</i> significantly increased body weight from 1 to 21 days (<i>p</i> < 0.05). In addition, the 10<sup>6</sup> CFU/kg <i>L. paracasei</i> LK01 group significantly reduced the activity of glutamic oxaloacetic transaminase and triglyceride levels; the 10<sup>7</sup> CFU/kg,10<sup>8</sup> CFU/kg, and 10<sup>9</sup> CFU/kg <i>L. paracasei</i> LK01 groups also reduced serum uric acid and total cholesterol levels (<i>p</i> < 0.05). The experimental groups all had lower serum levels of malondialdehyde and interleukin-1β (<i>p</i> < 0.01). Except for the 10<sup>6</sup> CFU/kg group, all experimental groups had significantly lower tumor necrosis factor-α, and the 10<sup>6</sup> and 10<sup>7</sup> CFU/kg groups had higher immunoglobulin M levels (<i>p</i> < 0.05). In addition, the 10<sup>6</sup> CFU/kg group significantly reduced the depth of the ileocecal crypts and increased the villus-to-crypt ratio (V/C) of the jejunum and ileum. In addition, dietary supplementation with <i>L. paracasei</i> LK01 did not change the α diversity of the microbial community in the cecum, but significantly increased the proportion of <i>Bacteroides</i> (phylum) (<i>p</i> < 0.05). The 10<sup>6</sup> CFU/kg group also significantly increased the abundance of beneficial bacteria such as <i>Ruminococcaceae</i> (genus), <i>Lachnospiraceae</i> (genus), and <i>Faecalibacterium</i> (genus) (<i>p</i> < 0.05). In summary, this study revealed that adding 10<sup>6</sup> CFU/kg of <i>L. paracasei</i> LKO1 to broiler diets can improve their production performance, serum biochemical indicators, antioxidant, and immune capabilities, as well as cecal flora.https://www.mdpi.com/2076-2615/14/23/3474<i>L. paracasei</i>broilergrowth performanceserum biochemical indicesserum antioxidantsimmune indices |
| spellingShingle | Weixin Liu Hong Cheng Hao Zhang Guozhen Liu Xinyu Yin Cheng Zhang Runsheng Jiang Zaigui Wang Xiaoling Ding Effect of <i>Lactobacillus paracasei</i> LK01 on Growth Performance, Antioxidant Capacity, Immunity, Intestinal Health, and Serum Biochemical Indices in Broilers Animals <i>L. paracasei</i> broiler growth performance serum biochemical indices serum antioxidants immune indices |
| title | Effect of <i>Lactobacillus paracasei</i> LK01 on Growth Performance, Antioxidant Capacity, Immunity, Intestinal Health, and Serum Biochemical Indices in Broilers |
| title_full | Effect of <i>Lactobacillus paracasei</i> LK01 on Growth Performance, Antioxidant Capacity, Immunity, Intestinal Health, and Serum Biochemical Indices in Broilers |
| title_fullStr | Effect of <i>Lactobacillus paracasei</i> LK01 on Growth Performance, Antioxidant Capacity, Immunity, Intestinal Health, and Serum Biochemical Indices in Broilers |
| title_full_unstemmed | Effect of <i>Lactobacillus paracasei</i> LK01 on Growth Performance, Antioxidant Capacity, Immunity, Intestinal Health, and Serum Biochemical Indices in Broilers |
| title_short | Effect of <i>Lactobacillus paracasei</i> LK01 on Growth Performance, Antioxidant Capacity, Immunity, Intestinal Health, and Serum Biochemical Indices in Broilers |
| title_sort | effect of i lactobacillus paracasei i lk01 on growth performance antioxidant capacity immunity intestinal health and serum biochemical indices in broilers |
| topic | <i>L. paracasei</i> broiler growth performance serum biochemical indices serum antioxidants immune indices |
| url | https://www.mdpi.com/2076-2615/14/23/3474 |
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