Detection of Mycobacterium tuberculosis from tongue swabs using sonication and sequence-specific hybridization capture.
Tongue swabs hold promise as a non-invasive sample for diagnosing tuberculosis (TB). However, their utility as replacements for sputum has been limited by their varied diagnostic performance in PCR assays compared to sputum. The use of silica-based DNA extraction methods may limit sensitivity due to...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0308235 |
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| author | Alexander J Yan Alaina M Olson Kris M Weigel Angelique K Luabeya Erin Heiniger Mark Hatherill Gerard A Cangelosi Paul Yager |
| author_facet | Alexander J Yan Alaina M Olson Kris M Weigel Angelique K Luabeya Erin Heiniger Mark Hatherill Gerard A Cangelosi Paul Yager |
| author_sort | Alexander J Yan |
| collection | DOAJ |
| description | Tongue swabs hold promise as a non-invasive sample for diagnosing tuberculosis (TB). However, their utility as replacements for sputum has been limited by their varied diagnostic performance in PCR assays compared to sputum. The use of silica-based DNA extraction methods may limit sensitivity due to incomplete lysis of Mycobacterium tuberculosis (MTB) cells and co-extraction of non-target nucleic acid, which may inhibit PCR. Specificity may also be compromised because these methods are labor-intensive and prone to cross-contamination. To address these limitations, we developed a sample preparation method that combines sonication for MTB lysis and a sequence-specific MTB DNA capture method using hybridization probes immobilized on magnetic beads. In spiked tongue swabs, our hybridization capture method demonstrated a 100-fold increase in MTB DNA yield over silica-based Qiagen DNA extraction and ethanol precipitation. In a study conducted on clinical samples from South Africa, our protocol had 74% (70/94) sensitivity and 98% (41/42) specificity for detecting active pulmonary TB with sputum Xpert MTB/RIF Ultra as the reference standard. While hybridization capture did not show improved sensitivity over Qiagen DNA extraction and ethanol precipitation, it demonstrated better specificity than previously reported methods and was easier to perform. With integration into point-of-care platforms, these strategies have the potential to help enable rapid non-sputum-based TB diagnosis across key underserved patient populations. |
| format | Article |
| id | doaj-art-c9f1fbd884e84e7ab2869fcc1567189d |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-c9f1fbd884e84e7ab2869fcc1567189d2024-12-10T05:32:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01198e030823510.1371/journal.pone.0308235Detection of Mycobacterium tuberculosis from tongue swabs using sonication and sequence-specific hybridization capture.Alexander J YanAlaina M OlsonKris M WeigelAngelique K LuabeyaErin HeinigerMark HatherillGerard A CangelosiPaul YagerTongue swabs hold promise as a non-invasive sample for diagnosing tuberculosis (TB). However, their utility as replacements for sputum has been limited by their varied diagnostic performance in PCR assays compared to sputum. The use of silica-based DNA extraction methods may limit sensitivity due to incomplete lysis of Mycobacterium tuberculosis (MTB) cells and co-extraction of non-target nucleic acid, which may inhibit PCR. Specificity may also be compromised because these methods are labor-intensive and prone to cross-contamination. To address these limitations, we developed a sample preparation method that combines sonication for MTB lysis and a sequence-specific MTB DNA capture method using hybridization probes immobilized on magnetic beads. In spiked tongue swabs, our hybridization capture method demonstrated a 100-fold increase in MTB DNA yield over silica-based Qiagen DNA extraction and ethanol precipitation. In a study conducted on clinical samples from South Africa, our protocol had 74% (70/94) sensitivity and 98% (41/42) specificity for detecting active pulmonary TB with sputum Xpert MTB/RIF Ultra as the reference standard. While hybridization capture did not show improved sensitivity over Qiagen DNA extraction and ethanol precipitation, it demonstrated better specificity than previously reported methods and was easier to perform. With integration into point-of-care platforms, these strategies have the potential to help enable rapid non-sputum-based TB diagnosis across key underserved patient populations.https://doi.org/10.1371/journal.pone.0308235 |
| spellingShingle | Alexander J Yan Alaina M Olson Kris M Weigel Angelique K Luabeya Erin Heiniger Mark Hatherill Gerard A Cangelosi Paul Yager Detection of Mycobacterium tuberculosis from tongue swabs using sonication and sequence-specific hybridization capture. PLoS ONE |
| title | Detection of Mycobacterium tuberculosis from tongue swabs using sonication and sequence-specific hybridization capture. |
| title_full | Detection of Mycobacterium tuberculosis from tongue swabs using sonication and sequence-specific hybridization capture. |
| title_fullStr | Detection of Mycobacterium tuberculosis from tongue swabs using sonication and sequence-specific hybridization capture. |
| title_full_unstemmed | Detection of Mycobacterium tuberculosis from tongue swabs using sonication and sequence-specific hybridization capture. |
| title_short | Detection of Mycobacterium tuberculosis from tongue swabs using sonication and sequence-specific hybridization capture. |
| title_sort | detection of mycobacterium tuberculosis from tongue swabs using sonication and sequence specific hybridization capture |
| url | https://doi.org/10.1371/journal.pone.0308235 |
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