Efficient Simultaneous Isolation of Pinostrobin and Panduratin A from <i>Boesenbergia rotunda</i> Using Centrifugal Partition Chromatography
The bioactive flavonoids pinostrobin (PN) and panduratin A (PA) from <i>Boesenbergia rotunda</i> are essential for research and therapeutic applications. This study introduces an innovative method utilizing ultrasound-assisted extraction with <i>n</i>-hexane pre-treatment, fo...
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MDPI AG
2024-11-01
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| Series: | Molecules |
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| author | Wanna Eiamart Supeecha Wittayalertpanya Sarin Tadtong Weerasak Samee |
| author_facet | Wanna Eiamart Supeecha Wittayalertpanya Sarin Tadtong Weerasak Samee |
| author_sort | Wanna Eiamart |
| collection | DOAJ |
| description | The bioactive flavonoids pinostrobin (PN) and panduratin A (PA) from <i>Boesenbergia rotunda</i> are essential for research and therapeutic applications. This study introduces an innovative method utilizing ultrasound-assisted extraction with <i>n</i>-hexane pre-treatment, followed by one-step centrifugal partition chromatography (CPC) purification. Extraction efficiency was evaluated using ultra high-performance liquid chromatography (UHPLC), and the isolated compounds were characterized through <sup>1</sup>H-NMR and liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), adhering to AOAC validation guidelines. Optimal extraction conditions comprised a particle size of 125 μm, a solid-to-liquid ratio of 1:30 g/mL, and a 10 min extraction time, yielding a crude extract of 6.96 ± 0.07%. Using an <i>n</i>-hexane/MeOH/water (5/3.4/1.6, <i>v</i>/<i>v</i>) solvent system in ascending mode, PN (2.16 mg, 98.78% purity) and PA (0.4 mg, 99.69% purity) were isolated from 67 mg of crude extract within 30 min. This streamlined approach enhances purification efficiency, allowing for faster extraction and higher purity, making it a suitable method for commercial applications. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-c9b830f59da3496a8e454b4c0d6e28582024-11-08T14:38:36ZengMDPI AGMolecules1420-30492024-11-012921518610.3390/molecules29215186Efficient Simultaneous Isolation of Pinostrobin and Panduratin A from <i>Boesenbergia rotunda</i> Using Centrifugal Partition ChromatographyWanna Eiamart0Supeecha Wittayalertpanya1Sarin Tadtong2Weerasak Samee3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, ThailandChula Pharmacokinetic Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, ThailandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, ThailandThe bioactive flavonoids pinostrobin (PN) and panduratin A (PA) from <i>Boesenbergia rotunda</i> are essential for research and therapeutic applications. This study introduces an innovative method utilizing ultrasound-assisted extraction with <i>n</i>-hexane pre-treatment, followed by one-step centrifugal partition chromatography (CPC) purification. Extraction efficiency was evaluated using ultra high-performance liquid chromatography (UHPLC), and the isolated compounds were characterized through <sup>1</sup>H-NMR and liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), adhering to AOAC validation guidelines. Optimal extraction conditions comprised a particle size of 125 μm, a solid-to-liquid ratio of 1:30 g/mL, and a 10 min extraction time, yielding a crude extract of 6.96 ± 0.07%. Using an <i>n</i>-hexane/MeOH/water (5/3.4/1.6, <i>v</i>/<i>v</i>) solvent system in ascending mode, PN (2.16 mg, 98.78% purity) and PA (0.4 mg, 99.69% purity) were isolated from 67 mg of crude extract within 30 min. This streamlined approach enhances purification efficiency, allowing for faster extraction and higher purity, making it a suitable method for commercial applications.https://www.mdpi.com/1420-3049/29/21/5186extractionpinostrobinpanduratin A<i>Boesenbergia rotunda</i>centrifugal partition chromatographypurification |
| spellingShingle | Wanna Eiamart Supeecha Wittayalertpanya Sarin Tadtong Weerasak Samee Efficient Simultaneous Isolation of Pinostrobin and Panduratin A from <i>Boesenbergia rotunda</i> Using Centrifugal Partition Chromatography Molecules extraction pinostrobin panduratin A <i>Boesenbergia rotunda</i> centrifugal partition chromatography purification |
| title | Efficient Simultaneous Isolation of Pinostrobin and Panduratin A from <i>Boesenbergia rotunda</i> Using Centrifugal Partition Chromatography |
| title_full | Efficient Simultaneous Isolation of Pinostrobin and Panduratin A from <i>Boesenbergia rotunda</i> Using Centrifugal Partition Chromatography |
| title_fullStr | Efficient Simultaneous Isolation of Pinostrobin and Panduratin A from <i>Boesenbergia rotunda</i> Using Centrifugal Partition Chromatography |
| title_full_unstemmed | Efficient Simultaneous Isolation of Pinostrobin and Panduratin A from <i>Boesenbergia rotunda</i> Using Centrifugal Partition Chromatography |
| title_short | Efficient Simultaneous Isolation of Pinostrobin and Panduratin A from <i>Boesenbergia rotunda</i> Using Centrifugal Partition Chromatography |
| title_sort | efficient simultaneous isolation of pinostrobin and panduratin a from i boesenbergia rotunda i using centrifugal partition chromatography |
| topic | extraction pinostrobin panduratin A <i>Boesenbergia rotunda</i> centrifugal partition chromatography purification |
| url | https://www.mdpi.com/1420-3049/29/21/5186 |
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