Function of vasohibin-2 and the mechanism of alternative splicing in triple-negative breast cancer
Objective·To explore the role of vasohibin-2 (VASH2) in the regulation of proliferation and metastasis of triple-negative breast cancer (TNBC) cells, and explore the mechanism of VASH2 in the occurrence and development of TNBC through regulation of gene expression and alternative splicing.Methods·TC...
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Editorial Office of Journal of Shanghai Jiao Tong University (Medical Science)
2024-12-01
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| Series: | Shanghai Jiaotong Daxue xuebao. Yixue ban |
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| Online Access: | https://xuebao.shsmu.edu.cn/article/2024/1674-8115/1674-8115-2024-44-12-1526.shtml |
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| author | WANG Wei WANG Hongli ALIBIYATI·i Ain YILIYAER· Rousu NUER AYI YANG Liang |
| author_facet | WANG Wei WANG Hongli ALIBIYATI·i Ain YILIYAER· Rousu NUER AYI YANG Liang |
| author_sort | WANG Wei |
| collection | DOAJ |
| description | Objective·To explore the role of vasohibin-2 (VASH2) in the regulation of proliferation and metastasis of triple-negative breast cancer (TNBC) cells, and explore the mechanism of VASH2 in the occurrence and development of TNBC through regulation of gene expression and alternative splicing.Methods·TCGA-GTEx was used to analyze the expression of VASH2 in TNBC. VASH2 methylation levels in TNBC were also analyzed. VASH2 was overexpressed in the MDA-MB-231 human TNBC cell line and transcriptome sequencing was performed. Differentially expressed genes and alternatively spliced genes regulated by VASH2 were analyzed to explore the mechanism of action of VASH2 in TNBC.Results·VASH2 was significantly overexpressed in TNBC compared to the normal tissues. Hypomethylation of the VASH2 gene was implicated in the upregulation of VASH2 expression in TNBC. Overexpression of VASH2 caused significant differential expression of 81 genes, of which 23 genes were up-regulated and 58 genes were down-regulated. Genes with significantly altered alternative splicing levels due to VASH2 overexpressed were enriched in cell cycle and p53 signaling pathways.Conclusion·VASH2 regulates the alternative splicing of TNBC oncogenes and promotes TNBC occurrence and development. |
| format | Article |
| id | doaj-art-c98408fcaa3747b2a7fcaf079f508d8c |
| institution | Kabale University |
| issn | 1674-8115 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Editorial Office of Journal of Shanghai Jiao Tong University (Medical Science) |
| record_format | Article |
| series | Shanghai Jiaotong Daxue xuebao. Yixue ban |
| spelling | doaj-art-c98408fcaa3747b2a7fcaf079f508d8c2025-01-08T05:52:54ZzhoEditorial Office of Journal of Shanghai Jiao Tong University (Medical Science)Shanghai Jiaotong Daxue xuebao. Yixue ban1674-81152024-12-0144121526153510.3969/j.issn.1674-8115.2024.12.0051674-8115（2024）12-1526-10Function of vasohibin-2 and the mechanism of alternative splicing in triple-negative breast cancerWANG Wei0WANG Hongli1ALIBIYATI·i Ain2YILIYAER· Rousu3NUER AYI4YANG Liang5Department of Head and Neck Surgery, Xinjiang Medical University Affiliated Cancer Hospital, Urumqi830011, ChinaDepartment of Head and Neck Surgery, Xinjiang Medical University Affiliated Cancer Hospital, Urumqi830011, ChinaDepartment of Head and Neck Surgery, Xinjiang Medical University Affiliated Cancer Hospital, Urumqi830011, ChinaDepartment of Head and Neck Surgery, Xinjiang Medical University Affiliated Cancer Hospital, Urumqi830011, ChinaDepartment of Head and Neck Surgery, Xinjiang Medical University Affiliated Cancer Hospital, Urumqi830011, ChinaDepartment of Head and Neck Surgery, Xinjiang Medical University Affiliated Cancer Hospital, Urumqi830011, ChinaObjective·To explore the role of vasohibin-2 (VASH2) in the regulation of proliferation and metastasis of triple-negative breast cancer (TNBC) cells, and explore the mechanism of VASH2 in the occurrence and development of TNBC through regulation of gene expression and alternative splicing.Methods·TCGA-GTEx was used to analyze the expression of VASH2 in TNBC. VASH2 methylation levels in TNBC were also analyzed. VASH2 was overexpressed in the MDA-MB-231 human TNBC cell line and transcriptome sequencing was performed. Differentially expressed genes and alternatively spliced genes regulated by VASH2 were analyzed to explore the mechanism of action of VASH2 in TNBC.Results·VASH2 was significantly overexpressed in TNBC compared to the normal tissues. Hypomethylation of the VASH2 gene was implicated in the upregulation of VASH2 expression in TNBC. Overexpression of VASH2 caused significant differential expression of 81 genes, of which 23 genes were up-regulated and 58 genes were down-regulated. Genes with significantly altered alternative splicing levels due to VASH2 overexpressed were enriched in cell cycle and p53 signaling pathways.Conclusion·VASH2 regulates the alternative splicing of TNBC oncogenes and promotes TNBC occurrence and development.https://xuebao.shsmu.edu.cn/article/2024/1674-8115/1674-8115-2024-44-12-1526.shtmlvasohibin-2 (vash2)triple-negative breast cancer (tnbc)alternative splicingp53 signaling pathwaycell cycle |
| spellingShingle | WANG Wei WANG Hongli ALIBIYATI·i Ain YILIYAER· Rousu NUER AYI YANG Liang Function of vasohibin-2 and the mechanism of alternative splicing in triple-negative breast cancer Shanghai Jiaotong Daxue xuebao. Yixue ban vasohibin-2 (vash2) triple-negative breast cancer (tnbc) alternative splicing p53 signaling pathway cell cycle |
| title | Function of vasohibin-2 and the mechanism of alternative splicing in triple-negative breast cancer |
| title_full | Function of vasohibin-2 and the mechanism of alternative splicing in triple-negative breast cancer |
| title_fullStr | Function of vasohibin-2 and the mechanism of alternative splicing in triple-negative breast cancer |
| title_full_unstemmed | Function of vasohibin-2 and the mechanism of alternative splicing in triple-negative breast cancer |
| title_short | Function of vasohibin-2 and the mechanism of alternative splicing in triple-negative breast cancer |
| title_sort | function of vasohibin 2 and the mechanism of alternative splicing in triple negative breast cancer |
| topic | vasohibin-2 (vash2) triple-negative breast cancer (tnbc) alternative splicing p53 signaling pathway cell cycle |
| url | https://xuebao.shsmu.edu.cn/article/2024/1674-8115/1674-8115-2024-44-12-1526.shtml |
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