<i>Saccharomyces cerevisiae</i>’s Response to Dysprosium Exposure
Lanthanide biosorption is important for recycling value-added materials. Previously, we analyzed dysprosium (Dy) absorption in screening strains of the unpopular yeast species <i>Schizoblastosporion</i> sp. However, it would be more desirable to use the well-known yeast <i>Saccharo...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
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| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/15/8/4426 |
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| Summary: | Lanthanide biosorption is important for recycling value-added materials. Previously, we analyzed dysprosium (Dy) absorption in screening strains of the unpopular yeast species <i>Schizoblastosporion</i> sp. However, it would be more desirable to use the well-known yeast <i>Saccharomyces cerevisiae</i> to make an easy-to-breed and efficient Dy-absorbing strain. Thus, we analyzed the physiological response and gene regulation of <i>S. cerevisiae</i> under Dy-absorbing conditions. The Dy content was measured using an inductively coupled plasma atomic emission spectrometer (ICP-AES). Transcriptional regulation was compared under Dy-absorbing and non-absorbing conditions through mRNA analysis and quantitative real-time polymerase chain reaction (qRT-PCR). In the yeast cells, approximately 40% of the Dy was located in the cell wall fraction, and the remaining 60% was located in the intracellular fraction. qRT-PCR analysis showed that the expression of four genes, <i>NCW2</i>, <i>PIR1</i>, <i>CRH1</i>, and <i>OLE1</i>, was upregulated, and that of <i>ATP14</i> was downregulated. These results suggest that <i>NCW2</i>, <i>PIR1</i>, and <i>CRH1</i> were responsible for cell wall rearrangement; <i>OLE1</i> initiated repair of the oxidative damage to the membrane lipids; and intracellular oxidation was caused by an imperfect <i>ATP14</i> product. |
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| ISSN: | 2076-3417 |