Optical sectioning methods in three-dimensional bioimaging
Abstract In recent advancements in life sciences, optical microscopy has played a crucial role in acquiring high-quality three-dimensional structural and functional information. However, the quality of 3D images is often compromised due to the intense scattering effect in biological tissues, compoun...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2025-01-01
|
| Series: | Light: Science & Applications |
| Online Access: | https://doi.org/10.1038/s41377-024-01677-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559095390240768 |
|---|---|
| author | Jing Zhang Wei Qiao Rui Jin Hongjin Li Hui Gong Shih-Chi Chen Qingming Luo Jing Yuan |
| author_facet | Jing Zhang Wei Qiao Rui Jin Hongjin Li Hui Gong Shih-Chi Chen Qingming Luo Jing Yuan |
| author_sort | Jing Zhang |
| collection | DOAJ |
| description | Abstract In recent advancements in life sciences, optical microscopy has played a crucial role in acquiring high-quality three-dimensional structural and functional information. However, the quality of 3D images is often compromised due to the intense scattering effect in biological tissues, compounded by several issues such as limited spatiotemporal resolution, low signal-to-noise ratio, inadequate depth of penetration, and high phototoxicity. Although various optical sectioning techniques have been developed to address these challenges, each method adheres to distinct imaging principles for specific applications. As a result, the effective selection of suitable optical sectioning techniques across diverse imaging scenarios has become crucial yet challenging. This paper comprehensively overviews existing optical sectioning techniques and selection guidance under different imaging scenarios. Specifically, we categorize the microscope design based on the spatial relationship between the illumination and detection axis, i.e., on-axis and off-axis. This classification provides a unique perspective to compare the implementation and performances of various optical sectioning approaches. Lastly, we integrate selected optical sectioning methods on a custom-built off-axis imaging system and present a unique perspective for the future development of optical sectioning techniques. |
| format | Article |
| id | doaj-art-c8bc282a81d24c669194ef8c1d7aa5d8 |
| institution | Kabale University |
| issn | 2047-7538 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Light: Science & Applications |
| spelling | doaj-art-c8bc282a81d24c669194ef8c1d7aa5d82025-01-05T12:47:00ZengNature Publishing GroupLight: Science & Applications2047-75382025-01-0114112810.1038/s41377-024-01677-xOptical sectioning methods in three-dimensional bioimagingJing Zhang0Wei Qiao1Rui Jin2Hongjin Li3Hui Gong4Shih-Chi Chen5Qingming Luo6Jing Yuan7Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyHong Kong Center for Cerebro-Cardiovascular Health Engineering, N.TBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyHong Kong Center for Cerebro-Cardiovascular Health Engineering, N.TBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyAbstract In recent advancements in life sciences, optical microscopy has played a crucial role in acquiring high-quality three-dimensional structural and functional information. However, the quality of 3D images is often compromised due to the intense scattering effect in biological tissues, compounded by several issues such as limited spatiotemporal resolution, low signal-to-noise ratio, inadequate depth of penetration, and high phototoxicity. Although various optical sectioning techniques have been developed to address these challenges, each method adheres to distinct imaging principles for specific applications. As a result, the effective selection of suitable optical sectioning techniques across diverse imaging scenarios has become crucial yet challenging. This paper comprehensively overviews existing optical sectioning techniques and selection guidance under different imaging scenarios. Specifically, we categorize the microscope design based on the spatial relationship between the illumination and detection axis, i.e., on-axis and off-axis. This classification provides a unique perspective to compare the implementation and performances of various optical sectioning approaches. Lastly, we integrate selected optical sectioning methods on a custom-built off-axis imaging system and present a unique perspective for the future development of optical sectioning techniques.https://doi.org/10.1038/s41377-024-01677-x |
| spellingShingle | Jing Zhang Wei Qiao Rui Jin Hongjin Li Hui Gong Shih-Chi Chen Qingming Luo Jing Yuan Optical sectioning methods in three-dimensional bioimaging Light: Science & Applications |
| title | Optical sectioning methods in three-dimensional bioimaging |
| title_full | Optical sectioning methods in three-dimensional bioimaging |
| title_fullStr | Optical sectioning methods in three-dimensional bioimaging |
| title_full_unstemmed | Optical sectioning methods in three-dimensional bioimaging |
| title_short | Optical sectioning methods in three-dimensional bioimaging |
| title_sort | optical sectioning methods in three dimensional bioimaging |
| url | https://doi.org/10.1038/s41377-024-01677-x |
| work_keys_str_mv | AT jingzhang opticalsectioningmethodsinthreedimensionalbioimaging AT weiqiao opticalsectioningmethodsinthreedimensionalbioimaging AT ruijin opticalsectioningmethodsinthreedimensionalbioimaging AT hongjinli opticalsectioningmethodsinthreedimensionalbioimaging AT huigong opticalsectioningmethodsinthreedimensionalbioimaging AT shihchichen opticalsectioningmethodsinthreedimensionalbioimaging AT qingmingluo opticalsectioningmethodsinthreedimensionalbioimaging AT jingyuan opticalsectioningmethodsinthreedimensionalbioimaging |