Minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy
BackgroundWhile widefield microscopy has long been constrained by out-of-focus scattering, advancements have generated a solution in the form of confocal laser scanning microscopy (cLSM) and optical sectioning microscopy using structured illumination (OSM). In this study, we aim to investigate, usin...
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Frontiers Media S.A.
2025-01-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnana.2024.1507140/full |
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| author | Sânziana Godeanu Sânziana Godeanu Mădălina Iuliana Mușat Anja Scheller Anja Scheller Eugen Osiac Bogdan Cătălin Bogdan Cătălin |
| author_facet | Sânziana Godeanu Sânziana Godeanu Mădălina Iuliana Mușat Anja Scheller Anja Scheller Eugen Osiac Bogdan Cătălin Bogdan Cătălin |
| author_sort | Sânziana Godeanu |
| collection | DOAJ |
| description | BackgroundWhile widefield microscopy has long been constrained by out-of-focus scattering, advancements have generated a solution in the form of confocal laser scanning microscopy (cLSM) and optical sectioning microscopy using structured illumination (OSM). In this study, we aim to investigate, using microglia branching, if cLSM and OSM can produce images with comparable morphological characteristics.ResultsBy imaging the somatosensory microglia from a tissue slice of a 3-week-old mouse and establishing morphological parameters that characterizes the microglial branching pattern, we were able to show that there is no difference in total length of the branch tree, number of branches, mean branch length and number of primary to terminal branches. We did find that area-based parameters such as mean occupied area and mean surveillance area were bigger in cLSM isolated microglia compared to OSM ones. Additionally, by investigating the difference in acquisition time between techniques and personal costs we were able to establish that the amortization could be made in 6.11 ± 2.93 years in the case of countries with a Human Development Index (HDI) = 7–9 and 7.06 ± 3.13 years, respectably, for countries with HDI < 7. As such, OSM systems seem a valid option if one just wants basic histological evaluation, and cLSM should be considered for groups that demand higher resolution or volumetric images. |
| format | Article |
| id | doaj-art-c89b6ce787924be79a0dfd69d68f4035 |
| institution | Kabale University |
| issn | 1662-5129 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neuroanatomy |
| spelling | doaj-art-c89b6ce787924be79a0dfd69d68f40352025-01-03T06:47:08ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292025-01-011810.3389/fnana.2024.15071401507140Minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopySânziana Godeanu0Sânziana Godeanu1Mădălina Iuliana Mușat2Anja Scheller3Anja Scheller4Eugen Osiac5Bogdan Cătălin6Bogdan Cătălin7Experimental Research Centre for Normal and Pathological Aging, University of Medicine and Pharmacy of Craiova, Craiova, RomaniaDepartment of Molecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland, Saarbrücken, GermanyExperimental Research Centre for Normal and Pathological Aging, University of Medicine and Pharmacy of Craiova, Craiova, RomaniaDepartment of Molecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland, Saarbrücken, GermanyCenter for Gender-Specific Biology and Medicine (CGBM), University of Saarland, Saarbrücken, GermanyDepartment of Biophysics, University of Medicine and Pharmacy of Craiova, Craiova, RomaniaExperimental Research Centre for Normal and Pathological Aging, University of Medicine and Pharmacy of Craiova, Craiova, RomaniaDepartment of Physiology, University of Medicine and Pharmacy of Craiova, Craiova, RomaniaBackgroundWhile widefield microscopy has long been constrained by out-of-focus scattering, advancements have generated a solution in the form of confocal laser scanning microscopy (cLSM) and optical sectioning microscopy using structured illumination (OSM). In this study, we aim to investigate, using microglia branching, if cLSM and OSM can produce images with comparable morphological characteristics.ResultsBy imaging the somatosensory microglia from a tissue slice of a 3-week-old mouse and establishing morphological parameters that characterizes the microglial branching pattern, we were able to show that there is no difference in total length of the branch tree, number of branches, mean branch length and number of primary to terminal branches. We did find that area-based parameters such as mean occupied area and mean surveillance area were bigger in cLSM isolated microglia compared to OSM ones. Additionally, by investigating the difference in acquisition time between techniques and personal costs we were able to establish that the amortization could be made in 6.11 ± 2.93 years in the case of countries with a Human Development Index (HDI) = 7–9 and 7.06 ± 3.13 years, respectably, for countries with HDI < 7. As such, OSM systems seem a valid option if one just wants basic histological evaluation, and cLSM should be considered for groups that demand higher resolution or volumetric images.https://www.frontiersin.org/articles/10.3389/fnana.2024.1507140/fullmicrogliacortexmorphologyoptical sectioning microscopeconfocal laser scanning microscopy |
| spellingShingle | Sânziana Godeanu Sânziana Godeanu Mădălina Iuliana Mușat Anja Scheller Anja Scheller Eugen Osiac Bogdan Cătălin Bogdan Cătălin Minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy Frontiers in Neuroanatomy microglia cortex morphology optical sectioning microscope confocal laser scanning microscopy |
| title | Minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy |
| title_full | Minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy |
| title_fullStr | Minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy |
| title_full_unstemmed | Minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy |
| title_short | Minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy |
| title_sort | minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy |
| topic | microglia cortex morphology optical sectioning microscope confocal laser scanning microscopy |
| url | https://www.frontiersin.org/articles/10.3389/fnana.2024.1507140/full |
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