Bromine indirubin FLIM/PLIM sensors to measure oxygen in normoxic and hypoxic PDT conditions
Background: The induction of phototoxicity during photodynamic therapy (PDT) is dependent on oxygen availability. For this reason, the development of sensors to measure oxygen and oxygen consumption is extremely important. Approach: In this project we have used Fluorescence Lifetime imaging (FLIM) a...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-02-01
|
| Series: | Photodiagnosis and Photodynamic Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100024000036 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846128276750204928 |
|---|---|
| author | D.N.S. dos Santos N. Naskar E. Delgado-Pinar K. Reess J.S. Seixas de Melo A. Rueck |
| author_facet | D.N.S. dos Santos N. Naskar E. Delgado-Pinar K. Reess J.S. Seixas de Melo A. Rueck |
| author_sort | D.N.S. dos Santos |
| collection | DOAJ |
| description | Background: The induction of phototoxicity during photodynamic therapy (PDT) is dependent on oxygen availability. For this reason, the development of sensors to measure oxygen and oxygen consumption is extremely important. Approach: In this project we have used Fluorescence Lifetime imaging (FLIM) and Phosphorescence Lifetime Imaging/ delayed Fluorescence Lifetime Imaging (PLIM/dFLIM) to investigate the ability of bromine indirubin derivatives as oxygen sensors. Results: The oxygen sensitivity of bromine indirubins was detected through PLIM/dFLIM. Moreover, we have observed, by measuring nicotinamide adenine dinucleotide (NADH) FLIM, that bromine indirubin has a significant impact on cellular metabolism by shifting the SCC-4 Cells metabolism from oxidative phosphorylation (OXPHOS) to glycolysis. Conclusions: In conclusion, this study successfully achieves its goals and provides important insights into the use of indirubin as a potential oxygen consumption sensor with the capability to identify and differentiate between normoxic and hypoxic regions within the cells. |
| format | Article |
| id | doaj-art-c8572d1e285543389f1089ddd0d7cd03 |
| institution | Kabale University |
| issn | 1572-1000 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-c8572d1e285543389f1089ddd0d7cd032024-12-11T05:55:33ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002024-02-0145103964Bromine indirubin FLIM/PLIM sensors to measure oxygen in normoxic and hypoxic PDT conditionsD.N.S. dos Santos0N. Naskar1E. Delgado-Pinar2K. Reess3J.S. Seixas de Melo4A. Rueck5University Ulm, Core Facility Confocal and Multiphoton Microscopy N24, Albert-Einstein-Allee 11, 89081 Ulm, Germany; University of Coimbra, CQC-ISM, Department of Chemistry, Coimbra, P3004-535, Portugal; Corresponding author.University Ulm, Core Facility Confocal and Multiphoton Microscopy N24, Albert-Einstein-Allee 11, 89081 Ulm, GermanyUniversity of Coimbra, CQC-ISM, Department of Chemistry, Coimbra, P3004-535, Portugal; Molecular Science Institute, Inorganic Chemistry Department, University of Valencia, C/Catedrático José Beltrán 2, Paterna 46980, Valencia, SpainUniversity Ulm, Core Facility Confocal and Multiphoton Microscopy N24, Albert-Einstein-Allee 11, 89081 Ulm, GermanyUniversity of Coimbra, CQC-ISM, Department of Chemistry, Coimbra, P3004-535, PortugalUniversity Ulm, Core Facility Confocal and Multiphoton Microscopy N24, Albert-Einstein-Allee 11, 89081 Ulm, GermanyBackground: The induction of phototoxicity during photodynamic therapy (PDT) is dependent on oxygen availability. For this reason, the development of sensors to measure oxygen and oxygen consumption is extremely important. Approach: In this project we have used Fluorescence Lifetime imaging (FLIM) and Phosphorescence Lifetime Imaging/ delayed Fluorescence Lifetime Imaging (PLIM/dFLIM) to investigate the ability of bromine indirubin derivatives as oxygen sensors. Results: The oxygen sensitivity of bromine indirubins was detected through PLIM/dFLIM. Moreover, we have observed, by measuring nicotinamide adenine dinucleotide (NADH) FLIM, that bromine indirubin has a significant impact on cellular metabolism by shifting the SCC-4 Cells metabolism from oxidative phosphorylation (OXPHOS) to glycolysis. Conclusions: In conclusion, this study successfully achieves its goals and provides important insights into the use of indirubin as a potential oxygen consumption sensor with the capability to identify and differentiate between normoxic and hypoxic regions within the cells.http://www.sciencedirect.com/science/article/pii/S1572100024000036NADH-FLIMPLIMdFLIMIndirubin derivativesUltrafast techniquesOxygen consumption |
| spellingShingle | D.N.S. dos Santos N. Naskar E. Delgado-Pinar K. Reess J.S. Seixas de Melo A. Rueck Bromine indirubin FLIM/PLIM sensors to measure oxygen in normoxic and hypoxic PDT conditions Photodiagnosis and Photodynamic Therapy NADH-FLIM PLIM dFLIM Indirubin derivatives Ultrafast techniques Oxygen consumption |
| title | Bromine indirubin FLIM/PLIM sensors to measure oxygen in normoxic and hypoxic PDT conditions |
| title_full | Bromine indirubin FLIM/PLIM sensors to measure oxygen in normoxic and hypoxic PDT conditions |
| title_fullStr | Bromine indirubin FLIM/PLIM sensors to measure oxygen in normoxic and hypoxic PDT conditions |
| title_full_unstemmed | Bromine indirubin FLIM/PLIM sensors to measure oxygen in normoxic and hypoxic PDT conditions |
| title_short | Bromine indirubin FLIM/PLIM sensors to measure oxygen in normoxic and hypoxic PDT conditions |
| title_sort | bromine indirubin flim plim sensors to measure oxygen in normoxic and hypoxic pdt conditions |
| topic | NADH-FLIM PLIM dFLIM Indirubin derivatives Ultrafast techniques Oxygen consumption |
| url | http://www.sciencedirect.com/science/article/pii/S1572100024000036 |
| work_keys_str_mv | AT dnsdossantos bromineindirubinflimplimsensorstomeasureoxygeninnormoxicandhypoxicpdtconditions AT nnaskar bromineindirubinflimplimsensorstomeasureoxygeninnormoxicandhypoxicpdtconditions AT edelgadopinar bromineindirubinflimplimsensorstomeasureoxygeninnormoxicandhypoxicpdtconditions AT kreess bromineindirubinflimplimsensorstomeasureoxygeninnormoxicandhypoxicpdtconditions AT jsseixasdemelo bromineindirubinflimplimsensorstomeasureoxygeninnormoxicandhypoxicpdtconditions AT arueck bromineindirubinflimplimsensorstomeasureoxygeninnormoxicandhypoxicpdtconditions |