MOLECULAR WEIGHT PROFILE OF PROTEASE OF PINEAPPLE (Ananas comosus L.Merr) AND PAPAYA (Carica papaya L.) USING SDS-PAGE METHOD
A group of protease enzymes such as papain and bromelain is able to decipher the molecular structure of the protein into amino acids which will be very useful in many fields, especially in food and pharmaceutical industries. The objective of this study to determine the molecular weight profile of en...
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Universitas Sebelas Maret
2017-03-01
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| Series: | Alchemy |
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| Online Access: | https://jurnal.uns.ac.id/alchemy/article/view/2540 |
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| author | Rizky Arcinthya Rachmania Priyo Wahyudi Aniza Mutia Wardania Dini Rohmatul Insania |
| author_facet | Rizky Arcinthya Rachmania Priyo Wahyudi Aniza Mutia Wardania Dini Rohmatul Insania |
| author_sort | Rizky Arcinthya Rachmania |
| collection | DOAJ |
| description | A group of protease enzymes such as papain and bromelain is able to decipher the molecular structure of the protein into amino acids which will be very useful in many fields, especially in food and pharmaceutical industries. The objective of this study to determine the molecular weight profile of enzyme bromelain from pineapple bark (Ananas comosus L. Merr) and papain (Carica papaya L.) from papaya latex with different varieties using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method. The precipitation was performed with ammonium sulfate ((NH4)2SO4)) 60 % addition and dialysized using a cellophane tube with a pore size of 12,000 Dalton. The molecular weight of enzyme solution were determined using SDS-PAGE. The analysis results of molecular weight of enzyme bromelain of Subang and Bogor varieties were not different and were about 30.654 kDa, as well as the molecular weight of enzyme papain and Sukma California varieties were also not different and were about 23.485 kDa. It can be concluded that the different varieties of fruit of pineapple and papaya had no effect on the molecular weight of enzyme papain and bromelain. |
| format | Article |
| id | doaj-art-c83d3d5465b9433680d7ae6a8731df4a |
| institution | Kabale University |
| issn | 1412-4092 2443-4183 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | Universitas Sebelas Maret |
| record_format | Article |
| series | Alchemy |
| spelling | doaj-art-c83d3d5465b9433680d7ae6a8731df4a2025-01-02T01:33:37ZengUniversitas Sebelas MaretAlchemy1412-40922443-41832017-03-01131526510.20961/alchemy.v13i1.2540MOLECULAR WEIGHT PROFILE OF PROTEASE OF PINEAPPLE (Ananas comosus L.Merr) AND PAPAYA (Carica papaya L.) USING SDS-PAGE METHODRizky Arcinthya Rachmania0Priyo Wahyudi1Aniza Mutia Wardania2Dini Rohmatul Insania 3Program Studi Farmasi, Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. Dr. HAMKA, Islamic Center, Jl. Delima II/IV Perumnas Klender, Jakarta Timur, 13460, Telp. (021) 8611070Badan Pengkajian dan Penerapan Teknologi, Gedung BPPT, Jl. MH Thamrin No. 8, Jakarta Pusat, Telp. (021) 3168200Program Studi Farmasi, Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. Dr. HAMKA, Islamic Center, Jl. Delima II/IV Perumnas Klender, Jakarta Timur, 13460, Telp. (021) 8611070Program Studi Farmasi, Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. Dr. HAMKA, Islamic Center, Jl. Delima II/IV Perumnas Klender, Jakarta Timur, 13460, Telp. (021) 8611070A group of protease enzymes such as papain and bromelain is able to decipher the molecular structure of the protein into amino acids which will be very useful in many fields, especially in food and pharmaceutical industries. The objective of this study to determine the molecular weight profile of enzyme bromelain from pineapple bark (Ananas comosus L. Merr) and papain (Carica papaya L.) from papaya latex with different varieties using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method. The precipitation was performed with ammonium sulfate ((NH4)2SO4)) 60 % addition and dialysized using a cellophane tube with a pore size of 12,000 Dalton. The molecular weight of enzyme solution were determined using SDS-PAGE. The analysis results of molecular weight of enzyme bromelain of Subang and Bogor varieties were not different and were about 30.654 kDa, as well as the molecular weight of enzyme papain and Sukma California varieties were also not different and were about 23.485 kDa. It can be concluded that the different varieties of fruit of pineapple and papaya had no effect on the molecular weight of enzyme papain and bromelain.https://jurnal.uns.ac.id/alchemy/article/view/2540bromelainpapainproteaseSDS-PAGE |
| spellingShingle | Rizky Arcinthya Rachmania Priyo Wahyudi Aniza Mutia Wardania Dini Rohmatul Insania MOLECULAR WEIGHT PROFILE OF PROTEASE OF PINEAPPLE (Ananas comosus L.Merr) AND PAPAYA (Carica papaya L.) USING SDS-PAGE METHOD Alchemy bromelain papain protease SDS-PAGE |
| title | MOLECULAR WEIGHT PROFILE OF PROTEASE OF PINEAPPLE (Ananas comosus L.Merr) AND PAPAYA (Carica papaya L.) USING SDS-PAGE METHOD |
| title_full | MOLECULAR WEIGHT PROFILE OF PROTEASE OF PINEAPPLE (Ananas comosus L.Merr) AND PAPAYA (Carica papaya L.) USING SDS-PAGE METHOD |
| title_fullStr | MOLECULAR WEIGHT PROFILE OF PROTEASE OF PINEAPPLE (Ananas comosus L.Merr) AND PAPAYA (Carica papaya L.) USING SDS-PAGE METHOD |
| title_full_unstemmed | MOLECULAR WEIGHT PROFILE OF PROTEASE OF PINEAPPLE (Ananas comosus L.Merr) AND PAPAYA (Carica papaya L.) USING SDS-PAGE METHOD |
| title_short | MOLECULAR WEIGHT PROFILE OF PROTEASE OF PINEAPPLE (Ananas comosus L.Merr) AND PAPAYA (Carica papaya L.) USING SDS-PAGE METHOD |
| title_sort | molecular weight profile of protease of pineapple ananas comosus l merr and papaya carica papaya l using sds page method |
| topic | bromelain papain protease SDS-PAGE |
| url | https://jurnal.uns.ac.id/alchemy/article/view/2540 |
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