DET induces apoptosis and suppresses tumor invasion in glioma cells via PI3K/AKT pathway
IntroductionGliomas, particularly glioblastomas (GBM), are highly aggressive with a poor prognosis and low survival rate. Currently, deoxyelephantopin (DET) has shown promising anti-inflammatory and anti-tumor effects. Using clinical prognostic analysis, molecular docking, and network pharmacology,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2024.1528454/full |
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| author | Rui Zhao Mengran Wang Zeyu Wu Panpan Zhao Huiling Dong Yue Su Chenghui Zhao Min Qi Shizhang Ling Shizhang Ling Xiaochun Jiang Xiaochun Jiang |
| author_facet | Rui Zhao Mengran Wang Zeyu Wu Panpan Zhao Huiling Dong Yue Su Chenghui Zhao Min Qi Shizhang Ling Shizhang Ling Xiaochun Jiang Xiaochun Jiang |
| author_sort | Rui Zhao |
| collection | DOAJ |
| description | IntroductionGliomas, particularly glioblastomas (GBM), are highly aggressive with a poor prognosis and low survival rate. Currently, deoxyelephantopin (DET) has shown promising anti-inflammatory and anti-tumor effects. Using clinical prognostic analysis, molecular docking, and network pharmacology, this study aims to explore the primary targets and signaling pathways to identify novel GBM treatment approaches.MethodsUsing PharmMapper, the chemical structure of DET was examined for possible targets after being acquired from PubChem. GBM-related targets were obtained through multi-omics approaches. A protein-protein interaction (PPI) network was constructed using Cytoscape and STRING, and target binding was evaluated through molecular docking. Enrichment analysis was conducted using Metascape. The effects of DET on GBM cell invasion, apoptosis, and proliferation were assessed through in vitro assays, including Transwell, EDU, CCK8, and flow cytometry. Western blot analysis was performed to examine the components of the PI3K/AKT signaling pathway.ResultsAmong the sixty-four shared targets identified, JUN and CCND1 were the most frequently observed. Enrichment analysis demonstrated that DET influenced the MAPK and PI3K/AKT signaling pathways. In Transwell assays, DET significantly inhibited the invasive behavior of glioma cells. Western blot analysis further confirmed the downregulation of EGFR, JUN, and PI3K/AKT.ConclusionDET inhibits GBM cell invasion, proliferation, and apoptosis via modulating the PI3K/AKT signaling pathway, highlighting its potential as a novel therapeutic strategy for GBM treatment. |
| format | Article |
| id | doaj-art-c83978b3d5e74e2fa7948f20a3f4fe07 |
| institution | Kabale University |
| issn | 2234-943X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj-art-c83978b3d5e74e2fa7948f20a3f4fe072025-01-09T14:18:15ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2025-01-011410.3389/fonc.2024.15284541528454DET induces apoptosis and suppresses tumor invasion in glioma cells via PI3K/AKT pathwayRui Zhao0Mengran Wang1Zeyu Wu2Panpan Zhao3Huiling Dong4Yue Su5Chenghui Zhao6Min Qi7Shizhang Ling8Shizhang Ling9Xiaochun Jiang10Xiaochun Jiang11The Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, Anhui, ChinaThe Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, Anhui, ChinaThe Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, Anhui, ChinaThe Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, Anhui, ChinaThe Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, Anhui, ChinaThe Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, Anhui, ChinaThe Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, Anhui, ChinaThe Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, Anhui, ChinaThe Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, Anhui, ChinaThe Institutes of Brain Science, Wannan Medical College, Higher Education Park, Wuhu, Anhui, ChinaThe Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, Anhui, ChinaThe Institutes of Brain Science, Wannan Medical College, Higher Education Park, Wuhu, Anhui, ChinaIntroductionGliomas, particularly glioblastomas (GBM), are highly aggressive with a poor prognosis and low survival rate. Currently, deoxyelephantopin (DET) has shown promising anti-inflammatory and anti-tumor effects. Using clinical prognostic analysis, molecular docking, and network pharmacology, this study aims to explore the primary targets and signaling pathways to identify novel GBM treatment approaches.MethodsUsing PharmMapper, the chemical structure of DET was examined for possible targets after being acquired from PubChem. GBM-related targets were obtained through multi-omics approaches. A protein-protein interaction (PPI) network was constructed using Cytoscape and STRING, and target binding was evaluated through molecular docking. Enrichment analysis was conducted using Metascape. The effects of DET on GBM cell invasion, apoptosis, and proliferation were assessed through in vitro assays, including Transwell, EDU, CCK8, and flow cytometry. Western blot analysis was performed to examine the components of the PI3K/AKT signaling pathway.ResultsAmong the sixty-four shared targets identified, JUN and CCND1 were the most frequently observed. Enrichment analysis demonstrated that DET influenced the MAPK and PI3K/AKT signaling pathways. In Transwell assays, DET significantly inhibited the invasive behavior of glioma cells. Western blot analysis further confirmed the downregulation of EGFR, JUN, and PI3K/AKT.ConclusionDET inhibits GBM cell invasion, proliferation, and apoptosis via modulating the PI3K/AKT signaling pathway, highlighting its potential as a novel therapeutic strategy for GBM treatment.https://www.frontiersin.org/articles/10.3389/fonc.2024.1528454/fullgliomacell proliferationcell apoptosisPl3Kcell invasion |
| spellingShingle | Rui Zhao Mengran Wang Zeyu Wu Panpan Zhao Huiling Dong Yue Su Chenghui Zhao Min Qi Shizhang Ling Shizhang Ling Xiaochun Jiang Xiaochun Jiang DET induces apoptosis and suppresses tumor invasion in glioma cells via PI3K/AKT pathway Frontiers in Oncology glioma cell proliferation cell apoptosis Pl3K cell invasion |
| title | DET induces apoptosis and suppresses tumor invasion in glioma cells via PI3K/AKT pathway |
| title_full | DET induces apoptosis and suppresses tumor invasion in glioma cells via PI3K/AKT pathway |
| title_fullStr | DET induces apoptosis and suppresses tumor invasion in glioma cells via PI3K/AKT pathway |
| title_full_unstemmed | DET induces apoptosis and suppresses tumor invasion in glioma cells via PI3K/AKT pathway |
| title_short | DET induces apoptosis and suppresses tumor invasion in glioma cells via PI3K/AKT pathway |
| title_sort | det induces apoptosis and suppresses tumor invasion in glioma cells via pi3k akt pathway |
| topic | glioma cell proliferation cell apoptosis Pl3K cell invasion |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2024.1528454/full |
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