Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis

Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis

Abstract Accurate and sensitive detection of simian malaria parasites is essential for surveillance and risk assessment of zoonotic malaria. We developed and validated a SYBR Green-based real-time PCR assay targeting the msp1 gene to detect and differentiate P. knowlesi, P. cynomolgi, and P. inui. S...

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Bibliographic Details
Main Authors: Thitiluck Swangsri, Rucksak Rucksaken, Wanat Sricharern, Wang Nguitragool, Naowarat Saralamba
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Subjects:
Multiplex PCR
Real-time PCR
Plasmodium
Plasmodium knowlesi
Plasmodium cynomolgi
Macaca fascicularis
Online Access:https://doi.org/10.1038/s41598-025-07337-3
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