Effect of Shorter Pretreatment Time on Clinical and Neonatal Outcomes in Human Blastocysts Vitrification after Artificial Shrinkage

Background: Prolonged pretreatment time may be harmful to frozen embryo’s developmental potential. This study was conducted to evaluate the effect of different equilibration times on the clinical and neonatal outcomes of frozen-warmed blastocyst transfer. Methods: This is a retrospective study based...

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Main Authors: Liuguang Zhang, Romualdo Sciorio, Yuhu Li, Ning Li
Format: Article
Language:English
Published: IMR Press 2023-09-01
Series:Clinical and Experimental Obstetrics & Gynecology
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Online Access:https://www.imrpress.com/journal/CEOG/50/9/10.31083/j.ceog5009197
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author Liuguang Zhang
Romualdo Sciorio
Yuhu Li
Ning Li
author_facet Liuguang Zhang
Romualdo Sciorio
Yuhu Li
Ning Li
author_sort Liuguang Zhang
collection DOAJ
description Background: Prolonged pretreatment time may be harmful to frozen embryo’s developmental potential. This study was conducted to evaluate the effect of different equilibration times on the clinical and neonatal outcomes of frozen-warmed blastocyst transfer. Methods: This is a retrospective study based on data collected from our medical records from March 2018 to March 2022 and including a total of 763 expanded blastocysts from 538 warming blastocyst cycles. These cycles were divided into two groups according to the equilibration time: (A) 6–7 minutes, and (B) 9–10 minutes. The survival rate, clinical, and neonatal outcomes were investigated. Results: The survival, implantation, and clinical pregnancy rates of vitrified-warmed shrinkage blastocyst were not different between the two groups. Other variables analyzed including live birth, multiple gestation, and neonatal outcomes were similar between the two groups. Conclusions: The results of this study illustrated that vitrification of artificially collapsed blastocysts with a shorter equilibration time (6–7 minutes) and pre-vitrification is able to lead to similar clinical and neonatal outcomes in patients undergoing assisted reproductive technology (ART).
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publishDate 2023-09-01
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series Clinical and Experimental Obstetrics & Gynecology
spelling doaj-art-c77c12a1b0ec4c5f91ccfba4d7280b7c2025-08-20T03:05:14ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632023-09-0150919710.31083/j.ceog5009197S0390-6663(23)02203-0Effect of Shorter Pretreatment Time on Clinical and Neonatal Outcomes in Human Blastocysts Vitrification after Artificial ShrinkageLiuguang Zhang0Romualdo Sciorio1Yuhu Li2Ning Li3Center for Reproductive Medicine, Haikou Mary Hospital, 570203 Haikou, Hainan, ChinaEdinburgh Assisted Conception Programme, Edinburgh Fertility Reproductive Endocrine Center (EFREC), Royal Infirmary of Edinburgh, EH16 4SA Edinburgh, UKCenter for Reproductive Medicine, Haikou Mary Hospital, 570203 Haikou, Hainan, ChinaCenter for Reproductive Medicine, Haikou Mary Hospital, 570203 Haikou, Hainan, ChinaBackground: Prolonged pretreatment time may be harmful to frozen embryo’s developmental potential. This study was conducted to evaluate the effect of different equilibration times on the clinical and neonatal outcomes of frozen-warmed blastocyst transfer. Methods: This is a retrospective study based on data collected from our medical records from March 2018 to March 2022 and including a total of 763 expanded blastocysts from 538 warming blastocyst cycles. These cycles were divided into two groups according to the equilibration time: (A) 6–7 minutes, and (B) 9–10 minutes. The survival rate, clinical, and neonatal outcomes were investigated. Results: The survival, implantation, and clinical pregnancy rates of vitrified-warmed shrinkage blastocyst were not different between the two groups. Other variables analyzed including live birth, multiple gestation, and neonatal outcomes were similar between the two groups. Conclusions: The results of this study illustrated that vitrification of artificially collapsed blastocysts with a shorter equilibration time (6–7 minutes) and pre-vitrification is able to lead to similar clinical and neonatal outcomes in patients undergoing assisted reproductive technology (ART).https://www.imrpress.com/journal/CEOG/50/9/10.31083/j.ceog5009197blastocyst vitrificationartificial collapseexposure to equilibration solutionpregnancy and implantation ratesspontaneous abortionneonatal outcomes
spellingShingle Liuguang Zhang
Romualdo Sciorio
Yuhu Li
Ning Li
Effect of Shorter Pretreatment Time on Clinical and Neonatal Outcomes in Human Blastocysts Vitrification after Artificial Shrinkage
Clinical and Experimental Obstetrics & Gynecology
blastocyst vitrification
artificial collapse
exposure to equilibration solution
pregnancy and implantation rates
spontaneous abortion
neonatal outcomes
title Effect of Shorter Pretreatment Time on Clinical and Neonatal Outcomes in Human Blastocysts Vitrification after Artificial Shrinkage
title_full Effect of Shorter Pretreatment Time on Clinical and Neonatal Outcomes in Human Blastocysts Vitrification after Artificial Shrinkage
title_fullStr Effect of Shorter Pretreatment Time on Clinical and Neonatal Outcomes in Human Blastocysts Vitrification after Artificial Shrinkage
title_full_unstemmed Effect of Shorter Pretreatment Time on Clinical and Neonatal Outcomes in Human Blastocysts Vitrification after Artificial Shrinkage
title_short Effect of Shorter Pretreatment Time on Clinical and Neonatal Outcomes in Human Blastocysts Vitrification after Artificial Shrinkage
title_sort effect of shorter pretreatment time on clinical and neonatal outcomes in human blastocysts vitrification after artificial shrinkage
topic blastocyst vitrification
artificial collapse
exposure to equilibration solution
pregnancy and implantation rates
spontaneous abortion
neonatal outcomes
url https://www.imrpress.com/journal/CEOG/50/9/10.31083/j.ceog5009197
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AT romualdosciorio effectofshorterpretreatmenttimeonclinicalandneonataloutcomesinhumanblastocystsvitrificationafterartificialshrinkage
AT yuhuli effectofshorterpretreatmenttimeonclinicalandneonataloutcomesinhumanblastocystsvitrificationafterartificialshrinkage
AT ningli effectofshorterpretreatmenttimeonclinicalandneonataloutcomesinhumanblastocystsvitrificationafterartificialshrinkage