Radioimmunoscintigraphy of Tumors Autocrine for Human Met and Hepatocyte Growth Factor/Scatter Factor
Inappropriate expression of the c-met-protooncogene product (Met) and/or of its ligand, hepatocyte growth factor/scatter factor (HGF/SF), has been correlated with poor prognosis in a variety of human solid tumors. We are developing animal models for nuclear imaging of Met and HGF/SF expression in tu...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2002-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200200006 |
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| author | Rick V. Hay Brian Cao R. Scot Skinner Ling-Mei Wang Yanli Su James H. Resau George F. Vande Woude Milton D. Gross |
| author_facet | Rick V. Hay Brian Cao R. Scot Skinner Ling-Mei Wang Yanli Su James H. Resau George F. Vande Woude Milton D. Gross |
| author_sort | Rick V. Hay |
| collection | DOAJ |
| description | Inappropriate expression of the c-met-protooncogene product (Met) and/or of its ligand, hepatocyte growth factor/scatter factor (HGF/SF), has been correlated with poor prognosis in a variety of human solid tumors. We are developing animal models for nuclear imaging of Met and HGF/SF expression in tumors in vivo. We radioiodinated a mixture of monoclonal antibodies (MAbs) that bind to human HGF/SF and to the external ligand-binding domain of human Met, and then injected the I-125-MAb mixture intravenously into mice bearing tumors either autocrine for human HGF/SF and human Met or autocrine-paracrine for murine HGF/SF and murine Met. Serial total body gamma camera images were obtained, and regional activity was determined by quantitative region-of-interest (ROI) analysis. Tumors autocrine for human HGF/SF and Met demonstrated significantly more rapid uptake and more rapid clearance of the I-125-MAb mixture than tumors expressing one or both murine homologues, reaching a mean tumor to total body activity ratio of > 0.3 by 1 day postinjection. We conclude that radioimmunodetection of tumors autocrine for human HGF/SF and Met is feasible with an I-125-MAb mixture reactive against the ligand-receptor pair. |
| format | Article |
| id | doaj-art-c7648bca380a4e5e9b018faa4df7ab6d |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2002-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-c7648bca380a4e5e9b018faa4df7ab6d2025-01-03T00:12:13ZengSAGE PublishingMolecular Imaging1536-01212002-01-01110.1162/1535350020020000610.1162_15353500200200006Radioimmunoscintigraphy of Tumors Autocrine for Human Met and Hepatocyte Growth Factor/Scatter FactorRick V. HayBrian CaoR. Scot SkinnerLing-Mei WangYanli SuJames H. ResauGeorge F. Vande WoudeMilton D. GrossInappropriate expression of the c-met-protooncogene product (Met) and/or of its ligand, hepatocyte growth factor/scatter factor (HGF/SF), has been correlated with poor prognosis in a variety of human solid tumors. We are developing animal models for nuclear imaging of Met and HGF/SF expression in tumors in vivo. We radioiodinated a mixture of monoclonal antibodies (MAbs) that bind to human HGF/SF and to the external ligand-binding domain of human Met, and then injected the I-125-MAb mixture intravenously into mice bearing tumors either autocrine for human HGF/SF and human Met or autocrine-paracrine for murine HGF/SF and murine Met. Serial total body gamma camera images were obtained, and regional activity was determined by quantitative region-of-interest (ROI) analysis. Tumors autocrine for human HGF/SF and Met demonstrated significantly more rapid uptake and more rapid clearance of the I-125-MAb mixture than tumors expressing one or both murine homologues, reaching a mean tumor to total body activity ratio of > 0.3 by 1 day postinjection. We conclude that radioimmunodetection of tumors autocrine for human HGF/SF and Met is feasible with an I-125-MAb mixture reactive against the ligand-receptor pair.https://doi.org/10.1162/15353500200200006 |
| spellingShingle | Rick V. Hay Brian Cao R. Scot Skinner Ling-Mei Wang Yanli Su James H. Resau George F. Vande Woude Milton D. Gross Radioimmunoscintigraphy of Tumors Autocrine for Human Met and Hepatocyte Growth Factor/Scatter Factor Molecular Imaging |
| title | Radioimmunoscintigraphy of Tumors Autocrine for Human Met and Hepatocyte Growth Factor/Scatter Factor |
| title_full | Radioimmunoscintigraphy of Tumors Autocrine for Human Met and Hepatocyte Growth Factor/Scatter Factor |
| title_fullStr | Radioimmunoscintigraphy of Tumors Autocrine for Human Met and Hepatocyte Growth Factor/Scatter Factor |
| title_full_unstemmed | Radioimmunoscintigraphy of Tumors Autocrine for Human Met and Hepatocyte Growth Factor/Scatter Factor |
| title_short | Radioimmunoscintigraphy of Tumors Autocrine for Human Met and Hepatocyte Growth Factor/Scatter Factor |
| title_sort | radioimmunoscintigraphy of tumors autocrine for human met and hepatocyte growth factor scatter factor |
| url | https://doi.org/10.1162/15353500200200006 |
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