Biodegradation of Azo and disperse dyes by Trametes versicolor: Process optimization and MnP enzyme dynamics
Textile industries are the major source of environmental pollution in the world because they release dyes, chemical and pigments in wastewater which are produced during dying process. The current study was designed with aims of screening Trametes versicolor biodegradation potential for four selected...
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Elsevier
2025-03-01
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| author | Isma Fayyaz Salina Saddick Raja Tahir Mahmood Muhammad Javaid Asad Muhammad Altaf Hussain Jiandong Hu Muhammad Awais M. Ijaz Khan Shaxnoza Saydaxmetova |
| author_facet | Isma Fayyaz Salina Saddick Raja Tahir Mahmood Muhammad Javaid Asad Muhammad Altaf Hussain Jiandong Hu Muhammad Awais M. Ijaz Khan Shaxnoza Saydaxmetova |
| author_sort | Isma Fayyaz |
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| description | Textile industries are the major source of environmental pollution in the world because they release dyes, chemical and pigments in wastewater which are produced during dying process. The current study was designed with aims of screening Trametes versicolor biodegradation potential for four selected textile dyes i.e. direct pink B, direct yellow BG, dispersed red S3B and disperse yellow SRLB. The process was further optimized by implying Response Surface Methodology (RSM) under Central Composite Design (CCD). The effect of manganese peroxidase (MnP) on the biodegradation was also monitored and it was partially purified as well as characterized. The screening experiments showed that this fungus has higher biodegradation potential for direct dyes (pink B = 58.9 % & yellow BG= 54.6 %) compared to disperse dyes (redS3B= 43.12 % & yellow SRLB= 27 %). The biodegradation of direct dyes was increased to 72.46 % & 93.29 % (pink B) and 84.69 % & 87.42 % (yellow BG) after two optimization steps. Study of manganese peroxidase (MnP) confirmed its active role in biodegradation with increasing its activity from 145 IU/mL/min to 194 IU/mL/min and from 188.5 IU/mL/min to 201.6 IU/mL/min during biodegradation of direct pink B & yellow BG, respectively. The MnP was partially purified in the presence of 60 % ammonium sulfate and it showed optimum pH= 4 and temperature 30 °C. The MnP of T. versicolor is quite active (Vmax= 60.24 mM/mL/min) ligninolytic enzyme and has good affinity for guaicol (Km=8.58 µM). |
| format | Article |
| id | doaj-art-c7628fdef70647eda3e734d42e40a4aa |
| institution | Kabale University |
| issn | 2590-1230 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Engineering |
| spelling | doaj-art-c7628fdef70647eda3e734d42e40a4aa2025-01-15T04:11:50ZengElsevierResults in Engineering2590-12302025-03-0125103980Biodegradation of Azo and disperse dyes by Trametes versicolor: Process optimization and MnP enzyme dynamicsIsma Fayyaz0Salina Saddick1Raja Tahir Mahmood2Muhammad Javaid Asad3Muhammad Altaf Hussain4Jiandong Hu5Muhammad Awais6M. Ijaz Khan7Shaxnoza Saydaxmetova8Department of Biotechnology, Mirpur University of Science and Technology (MUST), Mirpur 10250, AJK, PakistanDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Biotechnology, Mirpur University of Science and Technology (MUST), Mirpur 10250, AJK, Pakistan; Corresponding authors.University Institute of Biochemistry and Biotechnology, PMAS- Arid Agriculture University Rawalpindi, Pakistan; Corresponding authors.Department of Biotechnology, Mirpur University of Science and Technology (MUST), Mirpur 10250, AJK, PakistanDepartment of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; Henan International Joint Laboratory of Laser Technology in Agriculture Science, Zhengzhou 450002, China; State Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002, ChinaDepartment of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; Henan International Joint Laboratory of Laser Technology in Agriculture Science, Zhengzhou 450002, China; State Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002, ChinaDepartment of Mechanical Engineering, College of Engineering, Prince Mohammad Bin Fahd University, Al-Khobar, Saudi Arabia; Corresponding authors.Department of Chemistry and Its Teaching Methods, Tashkent State Pedagogical University, Tashkent, UzbekistanTextile industries are the major source of environmental pollution in the world because they release dyes, chemical and pigments in wastewater which are produced during dying process. The current study was designed with aims of screening Trametes versicolor biodegradation potential for four selected textile dyes i.e. direct pink B, direct yellow BG, dispersed red S3B and disperse yellow SRLB. The process was further optimized by implying Response Surface Methodology (RSM) under Central Composite Design (CCD). The effect of manganese peroxidase (MnP) on the biodegradation was also monitored and it was partially purified as well as characterized. The screening experiments showed that this fungus has higher biodegradation potential for direct dyes (pink B = 58.9 % & yellow BG= 54.6 %) compared to disperse dyes (redS3B= 43.12 % & yellow SRLB= 27 %). The biodegradation of direct dyes was increased to 72.46 % & 93.29 % (pink B) and 84.69 % & 87.42 % (yellow BG) after two optimization steps. Study of manganese peroxidase (MnP) confirmed its active role in biodegradation with increasing its activity from 145 IU/mL/min to 194 IU/mL/min and from 188.5 IU/mL/min to 201.6 IU/mL/min during biodegradation of direct pink B & yellow BG, respectively. The MnP was partially purified in the presence of 60 % ammonium sulfate and it showed optimum pH= 4 and temperature 30 °C. The MnP of T. versicolor is quite active (Vmax= 60.24 mM/mL/min) ligninolytic enzyme and has good affinity for guaicol (Km=8.58 µM).http://www.sciencedirect.com/science/article/pii/S2590123025000684Trametes versicolorDyes biodegradationResponse surface methodologyManganese peroxidase |
| spellingShingle | Isma Fayyaz Salina Saddick Raja Tahir Mahmood Muhammad Javaid Asad Muhammad Altaf Hussain Jiandong Hu Muhammad Awais M. Ijaz Khan Shaxnoza Saydaxmetova Biodegradation of Azo and disperse dyes by Trametes versicolor: Process optimization and MnP enzyme dynamics Results in Engineering Trametes versicolor Dyes biodegradation Response surface methodology Manganese peroxidase |
| title | Biodegradation of Azo and disperse dyes by Trametes versicolor: Process optimization and MnP enzyme dynamics |
| title_full | Biodegradation of Azo and disperse dyes by Trametes versicolor: Process optimization and MnP enzyme dynamics |
| title_fullStr | Biodegradation of Azo and disperse dyes by Trametes versicolor: Process optimization and MnP enzyme dynamics |
| title_full_unstemmed | Biodegradation of Azo and disperse dyes by Trametes versicolor: Process optimization and MnP enzyme dynamics |
| title_short | Biodegradation of Azo and disperse dyes by Trametes versicolor: Process optimization and MnP enzyme dynamics |
| title_sort | biodegradation of azo and disperse dyes by trametes versicolor process optimization and mnp enzyme dynamics |
| topic | Trametes versicolor Dyes biodegradation Response surface methodology Manganese peroxidase |
| url | http://www.sciencedirect.com/science/article/pii/S2590123025000684 |
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