Structural Characterization and Hypolipidemic Activity of Highland Barley Polysaccharide-Polyphenol Complexes
This study aimed to isolate a polysaccharide-polyphenol complex (HVP) from highland barley grains and investigate its potential role in regulating lipid metabolism. The structural properties of HVP were characterized in preliminary studies, and its phenolic components before and after hydrolysis wer...
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The editorial department of Science and Technology of Food Industry
2025-08-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080188 |
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| author | Zibin GUO Yang YANG Bei FAN Litao TONG Yatao HUANG Lili WANG Ting ZHANG Liya LIU Peipei SUN Jing SUN Fengzhong WANG |
| author_facet | Zibin GUO Yang YANG Bei FAN Litao TONG Yatao HUANG Lili WANG Ting ZHANG Liya LIU Peipei SUN Jing SUN Fengzhong WANG |
| author_sort | Zibin GUO |
| collection | DOAJ |
| description | This study aimed to isolate a polysaccharide-polyphenol complex (HVP) from highland barley grains and investigate its potential role in regulating lipid metabolism. The structural properties of HVP were characterized in preliminary studies, and its phenolic components before and after hydrolysis were qualitatively analyzed using a ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-TQMS-MS/MS) system. Furthermore, the lipid-lowering effects of HVP were assessed in a zebrafish hyperlipidemia model. The analysis revealed that HVP contained 494.4±13.0 mg/g of total sugars, 87.2±1.0 mg/g of total phenols, 47.6±1.0 mg/g of proteins, and 37.0±1.5 mg/g of uronic acids. The monosaccharide composition predominantly consisted of glucose and arabinose. Ferulic acid, apigenin, and hesperidin were identified in HVP before hydrolysis, while vanillin was identified as an additional component after hydrolysis. HVP markedly reduced oil red O staining in the caudal vasculature of zebrafish with hyperlipidemia (P<0.001). Additionally, HVP exhibited a dose-dependent effect on the reduction of triglyceride (TG) and total cholesterol (TC) levels in hyperlipidemic zebrafish, with the most pronounced effects observed at high doses (P<0.001). The structural characteristics and potential to regulate lipid metabolism of HVP provide new insights into its application in functional foods. |
| format | Article |
| id | doaj-art-c727da55d2e34c838ae888211fcf4b29 |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-08-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-c727da55d2e34c838ae888211fcf4b292025-08-20T03:47:03ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-08-01461537538210.13386/j.issn1002-0306.20240801882024080188-15Structural Characterization and Hypolipidemic Activity of Highland Barley Polysaccharide-Polyphenol ComplexesZibin GUO0Yang YANG1Bei FAN2Litao TONG3Yatao HUANG4Lili WANG5Ting ZHANG6Liya LIU7Peipei SUN8Jing SUN9Fengzhong WANG10College of Food Science and Engineering, Shanxi Agricultural University, Jinzhong 030801, ChinaInstitute of Western Agriculture, the Chinese Academy of Agricultural Sciences, Changji 831100, ChinaKey Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment on Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment on Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment on Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment on Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Jinzhong 030801, ChinaKey Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment on Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment on Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Jinzhong 030801, ChinaKey Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment on Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaThis study aimed to isolate a polysaccharide-polyphenol complex (HVP) from highland barley grains and investigate its potential role in regulating lipid metabolism. The structural properties of HVP were characterized in preliminary studies, and its phenolic components before and after hydrolysis were qualitatively analyzed using a ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-TQMS-MS/MS) system. Furthermore, the lipid-lowering effects of HVP were assessed in a zebrafish hyperlipidemia model. The analysis revealed that HVP contained 494.4±13.0 mg/g of total sugars, 87.2±1.0 mg/g of total phenols, 47.6±1.0 mg/g of proteins, and 37.0±1.5 mg/g of uronic acids. The monosaccharide composition predominantly consisted of glucose and arabinose. Ferulic acid, apigenin, and hesperidin were identified in HVP before hydrolysis, while vanillin was identified as an additional component after hydrolysis. HVP markedly reduced oil red O staining in the caudal vasculature of zebrafish with hyperlipidemia (P<0.001). Additionally, HVP exhibited a dose-dependent effect on the reduction of triglyceride (TG) and total cholesterol (TC) levels in hyperlipidemic zebrafish, with the most pronounced effects observed at high doses (P<0.001). The structural characteristics and potential to regulate lipid metabolism of HVP provide new insights into its application in functional foods.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080188highland barleypolysaccharide-polyphenol complexphenolic componentszebrafishlipid-lowering |
| spellingShingle | Zibin GUO Yang YANG Bei FAN Litao TONG Yatao HUANG Lili WANG Ting ZHANG Liya LIU Peipei SUN Jing SUN Fengzhong WANG Structural Characterization and Hypolipidemic Activity of Highland Barley Polysaccharide-Polyphenol Complexes Shipin gongye ke-ji highland barley polysaccharide-polyphenol complex phenolic components zebrafish lipid-lowering |
| title | Structural Characterization and Hypolipidemic Activity of Highland Barley Polysaccharide-Polyphenol Complexes |
| title_full | Structural Characterization and Hypolipidemic Activity of Highland Barley Polysaccharide-Polyphenol Complexes |
| title_fullStr | Structural Characterization and Hypolipidemic Activity of Highland Barley Polysaccharide-Polyphenol Complexes |
| title_full_unstemmed | Structural Characterization and Hypolipidemic Activity of Highland Barley Polysaccharide-Polyphenol Complexes |
| title_short | Structural Characterization and Hypolipidemic Activity of Highland Barley Polysaccharide-Polyphenol Complexes |
| title_sort | structural characterization and hypolipidemic activity of highland barley polysaccharide polyphenol complexes |
| topic | highland barley polysaccharide-polyphenol complex phenolic components zebrafish lipid-lowering |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080188 |
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