Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients
High levels of proinflammatory cytokines such as IFN-γ and TNF are associated with tissue lesions in cutaneous leishmaniasis (CL). We previously demonstrated that Schistosoma mansoni antigens downmodulate the in vitro cytokine response in CL. In the current study we evaluated whether S. mansoni anti...
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| Format: | Article |
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Wiley
2012-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2012/520308 |
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| author | Aline Michelle Barbosa Bafica Luciana Santos Cardoso Sérgio Costa Oliveira Alex Loukas Alfredo Góes Ricardo Riccio Oliveira Edgar M. Carvalho Maria Ilma Araujo |
| author_facet | Aline Michelle Barbosa Bafica Luciana Santos Cardoso Sérgio Costa Oliveira Alex Loukas Alfredo Góes Ricardo Riccio Oliveira Edgar M. Carvalho Maria Ilma Araujo |
| author_sort | Aline Michelle Barbosa Bafica |
| collection | DOAJ |
| description | High levels of proinflammatory cytokines such as IFN-γ and TNF are associated with tissue lesions in cutaneous leishmaniasis (CL). We previously demonstrated that Schistosoma mansoni antigens downmodulate the in vitro cytokine response in CL. In the current study we evaluated whether S. mansoni antigens alter monocyte and T-lymphocyte phenotypes in leishmaniasis. Peripheral blood mononuclear cells of CL patients were cultured with L. braziliensis antigen in the presence or absence of the S. mansoni antigens rSm29, rSmTSP-2- and PIII. Cells were stained with fluorochrome conjugated antibodies and analyzed by flow cytometry. The addition of rSm29 to the cultures decreased the expression of HLA-DR in nonclassical (CD14+CD16++) monocytes, while the addition of PIII diminished the expression of this molecule in classical (CD14++CD16-) and intermediate (CD14++CD16+) monocytes. The addition of PIII and rSmTSP-2 resulted in downmodulation of CD80 expression in nonclassical and CD86 expression in intermediate monocytes, respectively. These two antigens increased the expression of CTLA-4 in CD4+ T cells and they also expanded the frequency of CD4+CD25highFoxp3+ T cells. Taken together, we show that S. mansoni antigens, mainly rSmTSP-2 and PIII, are able to decrease the activation status of monocytes and also to upregulate the expression of modulatory molecules in T lymphocytes. |
| format | Article |
| id | doaj-art-c71f412fcbf14c2da827c2ebe693c47d |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-c71f412fcbf14c2da827c2ebe693c47d2025-02-03T05:47:36ZengWileyJournal of Parasitology Research2090-00232090-00312012-01-01201210.1155/2012/520308520308Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis PatientsAline Michelle Barbosa Bafica0Luciana Santos Cardoso1Sérgio Costa Oliveira2Alex Loukas3Alfredo Góes4Ricardo Riccio Oliveira5Edgar M. Carvalho6Maria Ilma Araujo7Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, 5 Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, 5 Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 6627 Avenida Antônio Carlos, Pampulha, 31270-901 Belo Horizonte, MG, BrazilQueensland Tropical Health Alliance and School of Public Health and Tropical Medicine, James Cook University, Cairns, QLD 4878, AustraliaDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 6627 Avenida Antônio Carlos, Pampulha, 31270-901 Belo Horizonte, MG, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, 5 Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, 5 Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, 5 Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilHigh levels of proinflammatory cytokines such as IFN-γ and TNF are associated with tissue lesions in cutaneous leishmaniasis (CL). We previously demonstrated that Schistosoma mansoni antigens downmodulate the in vitro cytokine response in CL. In the current study we evaluated whether S. mansoni antigens alter monocyte and T-lymphocyte phenotypes in leishmaniasis. Peripheral blood mononuclear cells of CL patients were cultured with L. braziliensis antigen in the presence or absence of the S. mansoni antigens rSm29, rSmTSP-2- and PIII. Cells were stained with fluorochrome conjugated antibodies and analyzed by flow cytometry. The addition of rSm29 to the cultures decreased the expression of HLA-DR in nonclassical (CD14+CD16++) monocytes, while the addition of PIII diminished the expression of this molecule in classical (CD14++CD16-) and intermediate (CD14++CD16+) monocytes. The addition of PIII and rSmTSP-2 resulted in downmodulation of CD80 expression in nonclassical and CD86 expression in intermediate monocytes, respectively. These two antigens increased the expression of CTLA-4 in CD4+ T cells and they also expanded the frequency of CD4+CD25highFoxp3+ T cells. Taken together, we show that S. mansoni antigens, mainly rSmTSP-2 and PIII, are able to decrease the activation status of monocytes and also to upregulate the expression of modulatory molecules in T lymphocytes.http://dx.doi.org/10.1155/2012/520308 |
| spellingShingle | Aline Michelle Barbosa Bafica Luciana Santos Cardoso Sérgio Costa Oliveira Alex Loukas Alfredo Góes Ricardo Riccio Oliveira Edgar M. Carvalho Maria Ilma Araujo Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients Journal of Parasitology Research |
| title | Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients |
| title_full | Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients |
| title_fullStr | Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients |
| title_full_unstemmed | Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients |
| title_short | Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients |
| title_sort | changes in t cell and monocyte phenotypes in vitro by schistosoma mansoni antigens in cutaneous leishmaniasis patients |
| url | http://dx.doi.org/10.1155/2012/520308 |
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