Genomic insights and metabolic profiling of gut commensal Luoshenia tenuis at strain level
Abstract Luoshenia tenuis, a newly identified gut commensal microbe from the family Christensenellaceae, has shown therapeutic effects on weight control and metabolic disorders in model mice. Bacterial strains are essential for investigations on the host-microbe interaction and further development o...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-08-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-025-00793-9 |
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| author | Xin-Wei Sun Hao-Jie Huang Yu-Zhi Zhao Hao-Yu Chen Chang-Yu Wang Zheng Zhou Yu Jiang Run-Yu Han He Jiang Chang Liu Shuang-Jiang Liu |
| author_facet | Xin-Wei Sun Hao-Jie Huang Yu-Zhi Zhao Hao-Yu Chen Chang-Yu Wang Zheng Zhou Yu Jiang Run-Yu Han He Jiang Chang Liu Shuang-Jiang Liu |
| author_sort | Xin-Wei Sun |
| collection | DOAJ |
| description | Abstract Luoshenia tenuis, a newly identified gut commensal microbe from the family Christensenellaceae, has shown therapeutic effects on weight control and metabolic disorders in model mice. Bacterial strains are essential for investigations on the host-microbe interaction and further development of medical applications. In this study, we collected 27 strains of L. tenuis from the Christensenellaceae Gut Microbial Biobank (ChrisGMB) and sequenced their complete genomes. Our analysis revealed considerable genetic diversity and genomic plasticity. Metabolic prediction indicated that L. tenuis had a preference for metabolizing plant-derived carbohydrates and the ability to synthesize various amino acids and cofactors. In silico analysis, along with in vitro experiments, validated that L. tenuis strains possessed strong acid tolerance and limited antibiotic resistance, suitable traits for oral probiotic development. Further volatile metabolomics and bile acid transformation profiling revealed that L. tenuis was capable of producing metabolites with previously-identified beneficial effects, along with extensive bile acid modification, potentially contributing to its positive impact on host metabolism. This study provides essential insight into strain-level functional and genomic features, laying a foundation for future research towards the development of L. tenuis-based therapies for metabolic disease. |
| format | Article |
| id | doaj-art-c716a2e2e3ed4de4a1616a7c3f9b19e4 |
| institution | Kabale University |
| issn | 2055-5008 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj-art-c716a2e2e3ed4de4a1616a7c3f9b19e42025-08-20T03:45:48ZengNature Portfolionpj Biofilms and Microbiomes2055-50082025-08-0111111210.1038/s41522-025-00793-9Genomic insights and metabolic profiling of gut commensal Luoshenia tenuis at strain levelXin-Wei Sun0Hao-Jie Huang1Yu-Zhi Zhao2Hao-Yu Chen3Chang-Yu Wang4Zheng Zhou5Yu Jiang6Run-Yu Han7He Jiang8Chang Liu9Shuang-Jiang Liu10State Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityAbstract Luoshenia tenuis, a newly identified gut commensal microbe from the family Christensenellaceae, has shown therapeutic effects on weight control and metabolic disorders in model mice. Bacterial strains are essential for investigations on the host-microbe interaction and further development of medical applications. In this study, we collected 27 strains of L. tenuis from the Christensenellaceae Gut Microbial Biobank (ChrisGMB) and sequenced their complete genomes. Our analysis revealed considerable genetic diversity and genomic plasticity. Metabolic prediction indicated that L. tenuis had a preference for metabolizing plant-derived carbohydrates and the ability to synthesize various amino acids and cofactors. In silico analysis, along with in vitro experiments, validated that L. tenuis strains possessed strong acid tolerance and limited antibiotic resistance, suitable traits for oral probiotic development. Further volatile metabolomics and bile acid transformation profiling revealed that L. tenuis was capable of producing metabolites with previously-identified beneficial effects, along with extensive bile acid modification, potentially contributing to its positive impact on host metabolism. This study provides essential insight into strain-level functional and genomic features, laying a foundation for future research towards the development of L. tenuis-based therapies for metabolic disease.https://doi.org/10.1038/s41522-025-00793-9 |
| spellingShingle | Xin-Wei Sun Hao-Jie Huang Yu-Zhi Zhao Hao-Yu Chen Chang-Yu Wang Zheng Zhou Yu Jiang Run-Yu Han He Jiang Chang Liu Shuang-Jiang Liu Genomic insights and metabolic profiling of gut commensal Luoshenia tenuis at strain level npj Biofilms and Microbiomes |
| title | Genomic insights and metabolic profiling of gut commensal Luoshenia tenuis at strain level |
| title_full | Genomic insights and metabolic profiling of gut commensal Luoshenia tenuis at strain level |
| title_fullStr | Genomic insights and metabolic profiling of gut commensal Luoshenia tenuis at strain level |
| title_full_unstemmed | Genomic insights and metabolic profiling of gut commensal Luoshenia tenuis at strain level |
| title_short | Genomic insights and metabolic profiling of gut commensal Luoshenia tenuis at strain level |
| title_sort | genomic insights and metabolic profiling of gut commensal luoshenia tenuis at strain level |
| url | https://doi.org/10.1038/s41522-025-00793-9 |
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