Prevalence of <i>Streptococcus mutans</i> and <i>Candida dubliniensis</i> in Plaque of Caries-free and Caries-active 3–6-year-old Children by Using Polymerase Chain Reaction: A Clinical Study
Introduction: Oral cavity harbors numerous types of microbial flora, which change frequently with changes in the environment and which in turn leads to the process of caries. Aims: To evaluate the prevalence of <i>Streptococus mutans (S. mutans)</i> and <i>Candida dubliniensis (C....
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Jaypee Brothers Medical Publisher
2022-12-01
|
| Series: | Journal of South Asian Association of Pediatric Dentistry |
| Subjects: | |
| Online Access: | https://www.jsaapd.com/doi/JSAAPD/pdf/10.5005/jp-journals-10077-3238 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Introduction: Oral cavity harbors numerous types of microbial flora, which change frequently with changes in the environment and which in turn leads to the process of caries.
Aims: To evaluate the prevalence of <i>Streptococus mutans (S. mutans)</i> and <i>Candida dubliniensis (C. dubliniensis)</i> in dental plaque of caries-free (CF) and carries-active (CA) children aged 3–6 years using polymerase chain reaction (PCR).
Material and methods: A total of 18 CA and CF children in the age group of 3–6 years were randomly selected. Plaque samples were collected using sterile micro brushes from teeth. Specific primers were used to carry out PCR in the plaque samples. Statistical analysis was done using Fisher's exact test.
Results: In CA group, <i>S. mutans</i> were seen in 61.1% of children which is statistically significant with a <i>p</i>-value of 0.04 and <i>C. dubliniensis</i> in 27.8% of children whereas, in CF group, <i>S. mutans</i> were present in 27.8% of participants and <i>C. dubliniensis</i> in 5.6%. These results show that both organisms were more predominant in the CA group.
Conclusion: Though both <i>S. mutans</i> (61.1%) and <i>C. dubliniensis</i> (27.8%) were present in CA group, <i>S. mutans</i> was strongly associated with dental caries. In the individuals with high scores of def and international caries detection and assessment system II, both species were more prevalent. |
|---|---|
| ISSN: | 2582-1024 |